Determination of yeast killer activity in fermenting sugarcane juice using selected ethanol-making strains

Ceccato-Antonini, Sandra Regina; Tosta, Christiann Davis; Silva, Ana Cláudia da

doi:10.1590/s1516-89132004000100003

Cited by 29 publications

(16 citation statements)

References 16 publications

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“…The fingerprinting technique used was ISSR (Inter Simple Sequence Repeats) with the primer GTG 5 , according to Silva-Filho et al (2005). Killer activity was also assessed in the isolates, according to Ceccato-Antonini et al (2004), using buffered YEPD-methylene blue at pH 4.5-4.7. Aliquots of 100 µL of the sensitive strains Saccharomyces cerevisiae NCYC 1006 and Torulopsis glabrata ATCC 15126 at cell suspensions of 4 X 10 5 cells/mL were spread on the culture medium.…”

Section: Methodsmentioning

confidence: 99%

Bioprospection of yeasts as biocontrol agents against phytopathogenic molds

Rosa-Magri

Tauk-Tornisielo

Ceccato-Antonini

2011

Braz. arch. biol. technol.

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Yeasts isolated from sugar cane and maize rhizosphere, leaves and stalks were screened against the phytopathogenic molds Colletotrichum sublineolum and Colletotrichum graminicola, both causal agents of the anthracnose disease in sorghum and maize, respectively. Strains identified as Torulaspora globosa and Candida intermedia were able to inhibit the mold growth, with the first species also exhibiting killer activity. No previous report on the application and potentiality of these yeasts as biocontrol agents were found neither the killer phenotype in Torulaspora globosa.

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Section: Methodsmentioning

confidence: 99%

Bioprospection of yeasts as biocontrol agents against phytopathogenic molds

Rosa-Magri

Tauk-Tornisielo

Ceccato-Antonini

2011

Braz. arch. biol. technol.

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“…The killer phenotype is based on the secretion of a low molecular mass protein or glycoprotein toxin able to kill sensitive cells belonging to the same or related yeast genera, but without direct cell-cell contact (Bevan and Makower, 1963). A Pichia strain (CCA 510) showed the best killer activity against 92% of isolated fermentative yeasts of the process (Ceccato-Antonini et al, 2004). De La Pena et al (1981) demonstrated that S. cerevisiae toxin was a protein which bond to a receptor on cell wall of sensitive yeasts, disrupting the electrochemical gradient across the cell membrane and hence the intracellular ionic balance.…”

Section: Resultsmentioning

confidence: 99%

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Coelho¹,

Celli

Ono

et al. 2007

Braz. arch. biol. technol.

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“…The killing activity was measured as the inhibition diameter around the well after incubation for 72 h at 25°C (CECCATO-ANTONINI et al, 2004).…”

Section: Screening Of the Isolated Yeasts For Killer Phenotypementioning

confidence: 99%

“…However, ethanol production is affected by undesirable yeasts from sugarcane and the environment. Killer yeasts may be a tool to reduce the effects caused by this microbial contaminant present in systems under non-sterile conditions (CECCATO-ANTONINI et al, 2004). Allied to the killer characteristic, ethanol tolerance is an important property for yeasts used in industrial ethanol production (TIKKA et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Isolamento, caracterização e identificação de leveduras killer de caldo de cana de açúcar

2015

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Ethanol produced by the fermentation of sugarcane juice has emerged as an important renewable fuel. The yield of this fermentation is affected by undesirable microbial contaminants, but killer yeasts can be a promising strategy to reduce this problem. The present study aimed to isolate, characterize, and identify wild killer yeasts from sugarcane juice. Samples were inoculated in culture medium containing chloramphenicol, and 140 colonies with different characteristics were selected. These isolates were submitted to the killer phenotype assay, and the positive killers were characterized and identified according to the standard methods. Only two strains showed killer activity, identified as Pichia anomala CE025 and P. membranaefaciens CE088. At 25°C, both strains exhibited killer activity at pH 4.0, 4.3, and 4.5, but this activity was not detected at pH 3.0, 3.5, 5.0, and 6.0. The killer phenotype of P. membranaefaciens CE088 was inhibited above 30°C, while for P. anomala, CE025 inhibition occurred only at a higher temperature. Both strains were able to grow in 12% ethanol, and P. anomala CE025 was more tolerant than P. membranaefaciens CE 088. Further studies will be conducted to isolate, purify and identify the killer toxins produced by Pichia anomala and Pichia membranaefaciens species.

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Determination of yeast killer activity in fermenting sugarcane juice using selected ethanol-making strains

Abstract: (0.07-0.18; 0.12-0.20; 0.10-0.13; 0.22-0.25 g/g, respectively) and biomass (0.19-0.26; 0.33-0.39; 0.13-0.27; 0.47-0.61 g/g, respectively) yields and fermentative efficiency (12.3-36.3; 21.0-40.0; 19.3-26.3; 47.6-54.0 %, respectively)

Cited by 29 publications

References 16 publications

Bioprospection of yeasts as biocontrol agents against phytopathogenic molds

Bioprospection of yeasts as biocontrol agents against phytopathogenic molds

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro

Isolamento, caracterização e identificação de leveduras killer de caldo de cana de açúcar

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