Determination of Xanthine Oxidase by Microcalorimetric Method

Abstract

I n t h i s study, the enzymatic oxidation o f acetaldehyde, one o f the s u b s t r a t e s of santhine oxidase, has been studied bya microcalorimetric method. A f l o w microcalorimeter i s used t o measure the h e a t changes during the reaction. The c a t a l y t i c determination of xanthine oxidase between the concentrations 0.2~10-1 and 2.4~10-l m g / m l (0.8~lO'~ -9.6fioe3 U / d ) has been achieved keeping acetaldehyde concentration constant at 4.0~10-~ I and the catalymetric determination of acetaldehyde between 0.8~lO'~ and 208fiO-~ M i s accomplished at a fixed concentration of enzyme.