Determination of water deficit in disks cut out from leaf blades

Čatský, J.

doi:10.1007/bf02920701

Cited by 115 publications

(20 citation statements)

References 5 publications

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“…Plant water status was estimated by RWC of leaf blades following the methodology described by Čatský (). Fresh weight (FW) was determined immediately after leaf sample collection, turgid weight (TW) was determined after saturating samples with milliQ grade water for 4 h, and dry weight (DW) measured after oven drying samples at 80 °C for 48 h.…”

Section: Methodsmentioning

confidence: 99%

Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus

Silvestre

Araújo

Patto

et al. 2014

JIPB

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Some species of the Lathyrus genus are among the most promising crops for marginal lands, with high resilience to drought, flood, and fungal diseases, combined with high yields and seed nutritional value. However, lack of knowledge on the mechanisms underlying its outstanding performance and methodologies to identify elite genotypes has hampered its proper use in breeding. Chlorophyll a fast fluorescence transient (JIP test), was used to evaluate water deficit (WD) resistance in Lathyrus genus. Our results reveal unaltered photochemical values for all studied genotypes showing resistance to mild WD. Under severe WD, two Lathyrus sativus genotypes showed remarkable resilience maintaining the photochemical efficiency, contrary to other genotypes studied. Performance index (PIABS) is the best parameter to screen genotypes with improved performance and grain production under WD. Moreover, we found that JIP indices are good indicators of genotypic grain production under WD. Quantum yield of electron transport (ϕEo) and efficiency with which trapped excitons can move electrons further than QA (ψ0) revealed as important traits related to improved photosynthetic performance and should be exploited in future Lathyrus germplasm improvements. The JIP test herein described showed to be an expeditious tool to screen and to identify elite genotypes with improved drought resistance.

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Section: Methodsmentioning

confidence: 99%

Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus

Silvestre

Araújo

Patto

et al. 2014

JIPB

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“…The relative water content for control leaves was 91.4%. The measure method of relative water content was described by Catsky (1960) [72]. …”

Section: Methodsmentioning

confidence: 99%

Identification of drought-responsive microRNAs in Medicago truncatula by genome-wide high-throughput sequencing

et al. 2011

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BackgroundMicroRNAs (miRNAs) are small, endogenous RNAs that play important regulatory roles in development and stress response in plants by negatively affecting gene expression post-transcriptionally. Identification of miRNAs at the global genome-level by high-throughout sequencing is essential to functionally characterize miRNAs in plants. Drought is one of the common environmental stresses limiting plant growth and development. To understand the role of miRNAs in response of plants to drought stress, drought-responsive miRNAs were identified by high-throughput sequencing in a legume model plant, Medicago truncatula.ResultsTwo hundreds eighty three and 293 known miRNAs were identified from the control and drought stress libraries, respectively. In addition, 238 potential candidate miRNAs were identified, and among them 14 new miRNAs and 15 new members of known miRNA families whose complementary miRNA*s were also detected. Both high-throughput sequencing and RT-qPCR confirmed that 22 members of 4 miRNA families were up-regulated and 10 members of 6 miRNA families were down-regulated in response to drought stress. Among the 29 new miRNAs/new members of known miRNA families, 8 miRNAs were responsive to drought stress with both 4 miRNAs being up- and down-regulated, respectively. The known and predicted targets of the drought-responsive miRNAs were found to be involved in diverse cellular processes in plants, including development, transcription, protein degradation, detoxification, nutrient status and cross adaptation.ConclusionsWe identified 32 known members of 10 miRNA families and 8 new miRNAs/new members of known miRNA families that were responsive to drought stress by high-throughput sequencing of small RNAs from M. truncatula. These findings are of importance for our understanding of the roles played by miRNAs in response of plants to abiotic stress in general and drought stress in particular.

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“…Ten leaf discs (1 cm diameter) per replicate, collected at 8:00 a.m. from the second fully expanded leaves (from the top), were used for quantifying the relative water content [RWC; Čatský (1960)]. For osmotic potential (Ψ s ) estimations, 10 leaf discs per replicate were also collected, flash frozen in liquid nitrogen and kept at −25 °C until use.…”

Section: Methodsmentioning

confidence: 99%

Salt Tolerance is Associated with Differences in Ion Accumulation, Biomass Allocation and Photosynthesis in Cowpea Cultivars

Praxedes

Lacerda

DaMatta

et al. 2010

J Agronomy Crop Science

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Cowpea is widely cultivated in arid and semi‐arid regions of the world where salinity is a major environmental stress that limits crop productivity. The effects of moderate salinity on growth and photosynthesis were examined during the vegetative phase of two cowpea cultivars previously classified as salt‐tolerant (Pitiúba) and salt‐sensitive (TVu). Two salt treatments (0 and 75 mm NaCl) were applied to 10‐day‐old plants grown in nutrient solution for 24 days. Salt stress caused decreases (59 % in Pitiúba and 72 % in TVu) in biomass accumulation at the end of the experiment. Photosynthetic rates per unit leaf mass, but not per unit leaf area, were remarkably impaired, particularly in TVu. This response was unlikely to have resulted from stomatal or photochemical constraints. Differences in salt tolerance between cultivars were unrelated to (i) variant patterns of Cl− and K+ tissue concentration, (ii) contrasting leaf water relations, or (iii) changes in relative growth rate and net assimilation rate. The relative advantage of Pitiúba over TVu under salt stress was primarily associated with (i) restricted Na+ accumulation in leaves paralleling an absolute increase in Na+ concentration in roots at early stages of salt treatment and (ii) improved leaf area (resulting from a larger leaf area ratio coupled with a larger leaf mass fraction and larger specific leaf area) and photosynthetic rates per unit leaf mass. Overall, these responses would allow greater whole‐plant carbon gain, thus contributing to a better agronomic performance of salt‐tolerant cowpea cultivars in salinity‐prone regions.

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Determination of water deficit in disks cut out from leaf blades

Cited by 115 publications

References 5 publications

Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus

Performance index: An expeditious tool to screen for improved drought resistance in the Lathyrus genus

Identification of drought-responsive microRNAs in Medicago truncatula by genome-wide high-throughput sequencing

Salt Tolerance is Associated with Differences in Ion Accumulation, Biomass Allocation and Photosynthesis in Cowpea Cultivars

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