Determination of vitamin C in tropical fruits: A comparative evaluation of methods

Hernández, Yurena; Lobo, M.; González, Mónica

doi:10.1016/j.foodchem.2005.04.012

Cited by 300 publications

(218 citation statements)

References 18 publications

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“…M a n u s c r i p t 8 To detect AA, ascorbate oxidase was immobilized in the microfluidic channel. The pH optimum for the enzymatic activity of ascorbate oxidase from Cucurbita sp.…”

Section: On-chip Ascorbic Acid Oxidationmentioning

confidence: 99%

“…[5] In food industry, AA is widely used as an antioxidant to avert the formation of carcinogenic nitrosamines in meat and sausage products. [6] The conventional detection methods for AA include titrimetric analysis, liquid chromatography [7,8], fluorometric assay [9], spectrophotometric determination, [10] and electrochemical techniques [11]. Titrimetric methods to AA analysis are simple, but usually need big volume of analyte solution.…”

Section: Introductionmentioning

confidence: 99%

“…Proteins, peptides, alcohol, caffeine, and color additives in food sources can also have absorbance near 266 nm that definitely interfere the determination of AA by UV/vis spectroscopy. Therefore, in complex matrices, such as fruit juice, separation of substrate samples is usually necessary, e. g. by ion-pair liquid chromatography [8], reverse phase high performance liquid chromatography [7], and capillary electrophoresis [12]. However, in the liquid chromatography based methods, the pretreatment of sample, the optimization of chromatographic conditions, and the choice of proper columns must be carefully considered.…”

Section: Introductionmentioning

confidence: 99%

“…However, in the liquid chromatography based methods, the pretreatment of sample, the optimization of chromatographic conditions, and the choice of proper columns must be carefully considered. [4,7,8] In the conventional capillary electrophoresis-based analysis, automated sampling may be timeconsuming and conflict with the instability of AA. [10] Escarpa et al have proposed microchip electrophoresis coupled with electrochemical detection for the separation and quantification of water-soluble vitamin.…”

Section: Introductionmentioning

confidence: 99%

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Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Fernandes

Cardoso

et al. 2016

Sensors and Actuators B: Chemical

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A microfluidic sensor is developed and targeted at specific ingredients determination in drug/food/beverage matrices. The surface of a serpentine polydimethylsiloxane (PDMS) microchannel is modified by enzyme via physisorption. When solutions containing target ingredients pass through the microfluidic channel, enzyme-catalyzed reaction occurs and only converts the target molecules to its products. The whole process is monitored by an end-channel UV/vis spectroscopic detection. Ascorbate oxidase and L-ascorbic acid (AA) are taken as enzyme-substrate model in this study to investigate the feasibility of using the developed strategy for direct quantification of AA in standard solutions and complex matrices. A dietary supplement product, vitamin C tablet, is chosen as a model matrix to test the microfluidic bio-sensor in real-sample analysis. The results illustrate that the established microfluidic biosensor exhibits good reproducibility, stability, and anti-interference property. Technically, it is easy to realize, depends on low investment in chip fabrication, and simple instrumental procedure, where only UV/vis spectrophotometer is required. To sum up, the developed strategy is economical, specific, and accurate, and can be potentially used for fast quantification of ingredient in samples with complex matrix background. It is promising to be widely spread in food industry and quality control department.

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“…M a n u s c r i p t 8 To detect AA, ascorbate oxidase was immobilized in the microfluidic channel. The pH optimum for the enzymatic activity of ascorbate oxidase from Cucurbita sp.…”

Section: On-chip Ascorbic Acid Oxidationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Fernandes

Cardoso

et al. 2016

Sensors and Actuators B: Chemical

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show abstract

“…An accurate and specific determination of vitamin C content on foodstuffs is extremely important to understand the relationship of dietary intake and human health [11]. To ensure that the subsequent analysis is effective, it is very important to optimize sample extraction when analyzing vitamin C in complex samples, such as fruits and vegetables [4,11].…”

Section: Introductionmentioning

confidence: 99%

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

et al. 2012

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This study provides a versatile validated method to determine the total vitamin C content, as the sum of the contents of L-ascorbic acid (L-AA) and dehydroascorbic acid (DHAA), in several fruits and vegetables and its degradability with storage time. Seven horticultural crops from two different origins were analyzed using an ultrahigh-performance liquid chromatographic-photodiode array (UHPLC-PDA) system, equipped with a new trifunctional high strength silica (100% silica particle) analytical column (100 mm×2.1 mm, 1.7 μm particle size) using 0.1% (v/v) formic acid as mobile phase, in isocratic mode. This new stationary phase, specially designed for polar compounds, overcomes the problems normally encountered in HPLC and is suitable for the analysis of large batches of samples without L-AA degradation. In addition, it proves to be an excellent alternative to conventional C18 columns for the determination of L-AA in fruits and vegetables. The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, accuracy, and inter/intraday precision. Validation experiments revealed very good recovery rate of 96.6±4.4% for L-AA and 103.1±4.8 % for total vitamin C, good linearity with r 2 -values >0.999 within the established concentration range, excellent repeatability (0.5%), and reproducibility (1.6%) values. The LOD of the method was 22 ng/mL whereas the LOQ was 67 ng/mL. It was possible to demonstrate that L-AA and DHAA concentrations in the different horticulture products varied oppositely with time of storage not always affecting the total amount of vitamin C during shelf-life. Locally produced fruits have higher concentrations of vitamin C, compared with imported ones, but vegetables showed the opposite trend. Moreover, this UHPLC-PDA methodology proves to be an improved, simple, and fast approach for determining the total content of vitamin C in various food commodities, with high sensitivity, selectivity, and resolving power within 3 min of run analysis.

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Bioactive compounds in fresh‐cut fruits

Pirovani

Piagentini

Velde

2015

Biotechnology of Bioactive Compounds

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Determination of vitamin C in tropical fruits: A comparative evaluation of methods

Cited by 300 publications

References 18 publications

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

Interference-blind microfluidic sensor for ascorbic acid determination by UV/vis spectroscopy

An improved and fast UHPLC-PDA methodology for determination of L-ascorbic and dehydroascorbic acids in fruits and vegetables. Evaluation of degradation rate during storage

Bioactive compounds in fresh‐cut fruits

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