Determination of Vitamin A in Feeds by HPLC with Anthracene as a Standard

Inno, Youichi; Ono, Tadayoshi

doi:10.2508/chikusan.58.813

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Vitamin A in the fat sample (n = 25 in above samples) was determined using HPLC with a modified procedure (Inno and Ono, 1987;Japanese Society for Food Science and Technology, 1996). The standards used were retinol and anthracene (Wako Chemical Company, Japan).…”

Section: Chemical Analysismentioning

confidence: 99%

Vitamins A and E in Carcass Fat from Japanese Black and F1 Cross Cattle

Irie¹,

Inno²,

Ishizuka³

et al. 2006

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

This study was carried out to determine the contents of vitamins A and E in carcass fat from Japanese Black and F1 cross cattle, because the method of controlling the amount of vitamin A in feed has been used in the production of expensive beef with high marbling in Japan. We determined the contents of tocopherol and retinol in subcutaneous and perirenal fats for 40 carcasses of Japanese Black and F1 (Holstein×Japanese Black crossed) cattle that had been sent to market from 10 prefectures. In bovine fat, free αtocopherol was present in the greatest amount, the level of γ-tocopherol was about 10% and there was only a trace of δ-tocopherol. The means±standard deviation of α-tocopherol content in subcutaneous and perirenal fats were 0.68±0.33 mg/100 g and 0.54±0.23 mg/100 g, respectively. The means±standard deviation of retinol content in subcutaneous and perirenal fats were 2.9±4.5 µg/100 g and 2.9±5.1 µg/100 g, respectively. Both vitamins differed widely between animals and were present at a relatively low level compared to other reports (α-tocopherol 2.4-5.4 mg/100 g fat tissue and 30-220 µg/100 g muscle tissue). The correlation coefficients between subcutaneous and perirenal fats for retinol and α-tocopherol content were 0.97 (p<0.01) and 0.82 (p<0.01), respectively, which showed high correlation. However, the correlation coefficients between retinol and α-tocopherol contents for subcutaneous and perirenal fats were 0.11 and 0.08, respectively, which showed low correlation. Breed effect was observed in both vitamins. These results indicated that vitamins A and E in fat from Japanese Black and F1 cross cattle under the vitamin A controlling method are present at relatively low levels and feeds need to be supplemented with vitamin E.

show abstract

Section: Chemical Analysismentioning

confidence: 99%