Determination of Visnagin inAmmi visnagaHairy Root Cultures Using Solid-Phase Extraction and High-Performance Liquid Chromatography

Kursinszki, L.; Troilina, J.; Szöke, Éva

doi:10.1006/mchj.1998.1590

Cited by 10 publications

(2 citation statements)

References 12 publications

(9 reference statements)

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“…The analysis of coumarins in A. visnaga is described by CE , TLC , and HPLC. The latter is definitely the preferred method, yet most of the published methods focus on single or a limited number of compounds only . What never has been reported before is the separation of the two position isomers dihydrosamidin and visnadin by LC.…”

Section: Introductionmentioning

confidence: 99%

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

2016

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The first supercritical fluid chromatography method for the determination of five major coumarins (dihydrosamidin, visnadin, samidin, khellin, and visnagin) in Ammi visnaga fruits is described. Their baseline separation was possible in less than 5 min by using a UPC HSS C SB column with 1.8 μm particle size and a mobile phase comprising CO , methanol, acetonitrile, and diethylamine. The type of stationary phase used was of particular relevance because, except for the selected one, the others did not resolve the two structural isomers dihydrosamidin and visnadin. Method validation confirmed that the procedure is linear (R ≥ 0.9996) in a concentration range from 6 to 480 μg/mL, it is accurate (recovery rates: 97.2-103.6%) and precise (intraday deviation ≤ 6.6%, intraday deviation ≤ 1.7%); injecting 1 μL of standard solution, the determined limit of detection was below 1.9 μg/mL for all compounds. The analysis of different A. visnaga samples revealed their similar compositions, and khellin (0.75-1.01%) and visnagin (0.18-0.46%) were the dominant coumarins. Visnadin and dihydrosamidin, the individual quantification of which is described for the first time, were present at concentrations below 0.14%.

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Section: Introductionmentioning

confidence: 99%

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

2016

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“…Various analytical methods have been used to identify and quantify visnagin, including thin-layer chromatography (TLC) [10,11] high performance liquid chromatography (HPLC) with solid-phase extraction (SPE) [12,10], capillary electrophoresis [13] and HPLC [14][15][16][17][18][19]. All of these methods involved multi-step extractions, large volumes of organic solvent and had large sample volume requirements.…”

Section: Introductionmentioning

confidence: 99%

Determination of visnagin in rat plasma by liquid chromatography with tandem mass spectrometry and its application to in vivo pharmacokinetic studies

Vanachayangkul

Butterweck

Frye

2009

Journal of Chromatography B

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Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

Tian

2015

Advances in Biochemical Engineering/Biotechnology

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Plants synthesize a wide variety of natural products, which are traditionally termed secondary metabolites and, more recently, coined specialized metabolites. While these chemical compounds are employed by plants for interactions with their environment, humans have long since explored and exploited plant secondary metabolites for medicinal and practical uses. Due to the tissue-specific and low-abundance accumulation of these metabolites, alternative means of production in systems other than intact plants are sought after. To this end, hairy root culture presents an excellent platform for producing valuable secondary metabolites. This chapter will focus on several major groups of secondary metabolites that are manufactured by hairy roots established from different plant species. Additionally, the methods for preservations of hairy roots will also be reviewed.

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Determination of Visnagin inAmmi visnagaHairy Root Cultures Using Solid-Phase Extraction and High-Performance Liquid Chromatography

Cited by 10 publications

References 12 publications

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

Fast and improved separation of major coumarins inAmmi visnaga(L.) Lam. by supercritical fluid chromatography

Determination of visnagin in rat plasma by liquid chromatography with tandem mass spectrometry and its application to in vivo pharmacokinetic studies

Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

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