Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

Tian, Li

doi:10.1007/10_2014_298

Cited by 34 publications

(20 citation statements)

References 185 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study hypocotyls were found to be the most responsive tissue for infection. The hairy root system is very interesting for the production of secondary metabolites in medicinal plants ( Jiao et al, 2014 ; Patra and Srivastava, 2014 ; Wawrosch et al, 2014 ; Gai et al, 2015 ; Tian, 2015 ) or to engineer model plants to secrete industrially valuable metabolites. For example, in tobacco transgenic hairy roots the production of THCA was successfully obtained by expressing hemp THCAS ( Sirikantaramas et al, 2007 ).…”

Section: Cannabis Biotechnology: Challenges and Prospectsmentioning

confidence: 99%

Cannabis sativa: The Plant of the Thousand and One Molecules

2016

View full text Add to dashboard Cite

Cannabis sativa L. is an important herbaceous species originating from Central Asia, which has been used in folk medicine and as a source of textile fiber since the dawn of times. This fast-growing plant has recently seen a resurgence of interest because of its multi-purpose applications: it is indeed a treasure trove of phytochemicals and a rich source of both cellulosic and woody fibers. Equally highly interested in this plant are the pharmaceutical and construction sectors, since its metabolites show potent bioactivities on human health and its outer and inner stem tissues can be used to make bioplastics and concrete-like material, respectively. In this review, the rich spectrum of hemp phytochemicals is discussed by putting a special emphasis on molecules of industrial interest, including cannabinoids, terpenes and phenolic compounds, and their biosynthetic routes. Cannabinoids represent the most studied group of compounds, mainly due to their wide range of pharmaceutical effects in humans, including psychotropic activities. The therapeutic and commercial interests of some terpenes and phenolic compounds, and in particular stilbenoids and lignans, are also highlighted in view of the most recent literature data. Biotechnological avenues to enhance the production and bioactivity of hemp secondary metabolites are proposed by discussing the power of plant genetic engineering and tissue culture. In particular two systems are reviewed, i.e., cell suspension and hairy root cultures. Additionally, an entire section is devoted to hemp trichomes, in the light of their importance as phytochemical factories. Ultimately, prospects on the benefits linked to the use of the -omics technologies, such as metabolomics and transcriptomics to speed up the identification and the large-scale production of lead agents from bioengineered Cannabis cell culture, are presented.

show abstract

Section: Cannabis Biotechnology: Challenges and Prospectsmentioning

confidence: 99%

Cannabis sativa: The Plant of the Thousand and One Molecules

2016

View full text Add to dashboard Cite

show abstract

“…Plants usually produce or accumulate very low quantities of metabolites of interest and large quantities of plant material are necessary to obtain an active substance, so in this sense hairy roots represent an alternative to overcome this problem and to produce higher amounts of secondary metabolites, or recently known specialized metabolites [ 1 ]. In recent years, several workers have reported the use of hairy root cultures for the production of secondary metabolites such as silymarin, a mixture of flavonolignans with hepatoprotective properties from hairy root cultures of Sylibum marianum [ 2 ], methyllycaconitine, a potential drug for the treatment of Alzheimer´s disease from mutagenesized hairy root cultures of Solidago memoralis [ 3 ], podophyllotoxin and 6-methoxy-podophyllotoxin from hairy roots of Linum mucronatum [ 4 ], and also the production of primary metabolites such as polyunsaturated fatty acids from hairy root cultures of Echium acanthocarpum [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae)

et al. 2017

View full text Add to dashboard Cite

The genetically transformed hairy root line LRT 7.31 obtained by infecting leaf explants of Lopezia racemosa Cav with the Agrobacterium rhizogenes strain ATCC15834/pTDT, was evaluated to identify the anti-inflammatory and cytotoxic compounds reported previously for the wild plant. After several subcultures of the LRT 7.31 line, the bio-guided fractionation of the dichloromethane–methanol (1:1) extract obtained from dry biomass afforded a fraction that showed important in vivo anti-inflammatory, and in vitro cytotoxic activities. Chemical separation of the active fraction allowed us to identify the triterpenes ursolic (1) and oleanolic (2) acids, and (23R)-2α,3β,23,28-tetrahydroxy-14,15-dehydrocampesterol (3) as the anti-inflammatory principles of the active fraction. A new molecule 3 was characterized by spectroscopic analysis of its tetraacetate derivative 3a. This compound was not described in previous reports of callus cultures, in vitro germinated seedlings and wild plant extracts of whole L. racemosa plants. The anti-inflammatory and cytotoxic activities displayed by the fraction are associated to the presence of compounds 1–3. The present study reports the obtaining of the transformed hairy roots, the bioguided isolation of the new molecule 3, and its structure characterization.

show abstract

“…2006). The production of secondary metabolites in hairy root cultures is influenced by many factors, including temperature, light, pH, medium composition, and plant growth regulators (Zhou et al, 2011;Tian, 2015). Hairy root cultures from many plant species have been widely studied for the production of secondary metabolites useful in pharmaceuticals, cosmetics, and food additives (Srivastava and Srivastava, 2007;Ludwig-Müller et al, 2014;Maschke et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Glucosinolate biosynthesis as influenced by growth media and auxin in hairy root cultures of Kale (Brassica oleracea var. acephala)

Lee

Bong

Kim

et al. 2016

Emir. J. Food Agric

View full text Add to dashboard Cite

This study aimed to investigate the effects of various growth media and three types of auxins on glucosinolate biosynthesis in hairy root cultures of kale (Brassica oleracea var. acephala). Four different glucosinolates (4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin) were used in this study. The accumulation of glucosinolates was influenced by both media and auxin treatments. Of the media treatments, full-strength Gamborg's B5 medium (B5) supported the highest accumulation of total glucosinolates, followed by full-strength Murashige-Skoog medium (MS), whereas the lowest glucosinolates accumulation was in the half-strength MS medium treatment. The accumulation of glucobrassicin was very high, followed by 4-methoxyglucobrassicin, irrespective of the growth medium used. The highest content of glucobrassicin was measured in cultures in B5 medium, while MS medium resulted in the highest accumulation of 4-methoxyglucobrassicin. Half-strength B5 medium resulted in the highest content of neoglucobrassicin, and Schenk and Hildebrandt medium (SH) supported the highest content of 4-hydroxyglucobrassicin. The total and individual levels of glucosinolates in hairy root cultures of kale were all influenced by exposure to the nine auxin treatments, with the exception glucobrassicin. In general, levels of glucosinolate decreased with increasing concentrations of auxins. Treatment with the auxin indole-3-butyric acid (IBA) 0.1 resulted in the highest total concentration of glucosinolates, measuring 1.83 times higher than that of hairy root cultures treated with naphthalene acetic acid (NAA) 1.0, which resulted in the lowest glucosinolate concentrations. Of the four glucosinolates, glucobrassicin and 4-methoxyglucobrassicin contents were considerably higher. The auxin IBA 0.1 promoted the highest 4-methoxyglucobrassicin accumulation in the hairy root cultures of kale. The auxin treatment indole-3-acetic acid (IAA) 0.1 resulted in the highest amount of 4-hydroxyglucobrassicin and neoglucobrassicin. Hairy root cultures could be a valuable alternative approach for the production of glucosinolate compounds from kale.

show abstract

Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

Cited by 34 publications

References 185 publications

Cannabis sativa: The Plant of the Thousand and One Molecules

Cannabis sativa: The Plant of the Thousand and One Molecules

A Cytotoxic and Anti-inflammatory Campesterol Derivative from Genetically Transformed Hairy Roots of Lopezia racemosa Cav. (Onagraceae)

Glucosinolate biosynthesis as influenced by growth media and auxin in hairy root cultures of Kale (Brassica oleracea var. acephala)

Contact Info

Product

Resources

About