Determination of valdecoxib and its metabolites in human urine by automated solid-phase extraction–liquid chromatography–tandem mass spectrometry

Automated liquid chromatography–tandem mass spectrometry method for the analysis of firocoxib in urine and plasma from horse and dog

“…Numerous methods for the quantitation of COX-2 inhibitors in biological matrices utilizing LC separation with UV [8][9][10], fluorescence [11,12], and MS [5,[13][14][15][16][17][18][19] detection have been published, a small number of which are referenced here. Many of these methods were summarized in a review paper by Nageswara Rao et al [20].…”

New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications

“…With liquid-liquid extraction it is difficult to obtain a high recovery of polar analytes and it is not easy to automate the methods. Solid-phase extraction (SPE) gives both high recovery and good chromatography, but takes longer and more steps are required [1][2][3][4][5][6]. SPME as sample preparation has become a popular microextraction technique.…”

Fast LC/MS in the analysis of small molecules

“…Fully automated 96-well methods have been put into operation for liquid-liquid extractions [22,165] and liquid-solid extractions [166]. Fully automated SPE is the most prevalent form of automation and has been used for the analysis of multiple species plasma and urine samples [80,142,143,144,145,167,168,169] and in conjunction with cassette-dosed pharmacokinetic animal studies [170]. Barbarin et al [171], use automated 96-well rat plasma protein precipitation on a monolith column at 3.5 mL min -1 for a 17-s injection-to-injection analysis.…”