New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications

Abdel‐Rehim, Mohamed

doi:10.1016/j.jchromb.2003.11.042

Cited by 355 publications

(100 citation statements)

References 14 publications

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“…Any sorbent material such as silica-based (C2, C8, C18), strong cation exchanger (SCX) using sulfonic acid-bonded silica, restricted access material, HILIC, carbon, polystyrenedivinylbenzene copolymer, or molecularly imprinted polymers, can be used. This technique has been used to extract a wide range of analytes including drugs from biological samples [31][32][33][34], polycyclic aromatic hydrocarbons, and semi-volatile residues in water [35,36], analysis of small brominated and chlorinated aromatic compounds in wine [37], flavonoid content in fruit juice [38], phenolic anti-oxidants from cereal products [39], and environment pollutants [40].…”

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confidence: 99%

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

Gonçalves

Câmara

2011

J of Separation Science

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An ultra-fast and improved analytical methodology based on microextraction by packed sorbent (MEPS) combined with ultra-performance LC (UPLC) was developed and validated for determination of (E)-resveratrol in wines. Important factors affecting the performance of MEPS such as the type of sorbent material (C2, C8, C18, SIL, and M1), number of extraction cycles, and sample volume were studied. The optimal conditions of MEPS extraction were obtained using C8 sorbent and small sample volumes (50-250 mL) in one extraction cycle (extract-discard) and in a short time period (about 3 min for the entire sample preparation step). (E)-Resveratrol was eluted by 1 Â 250 mL of the mixture containing 95% methanol and 5% water, and the separation was carried out on a highstrength silica HSS T3 analytical column (100 mm Â 2.1 mm, 1.8 mm particle size) using a binary mobile phase composed of aqueous 0.1% formic acid (eluent A) and methanol (eluent B) in the gradient elution mode (10 min of total analysis). The method was fully validated in terms of linearity, detection (LOD) and quantification (LOQ) limits, extraction yield, accuracy, and inter/intra-day precision, using a Madeira wine sample (ET) spiked with (E)-resveratrol at concentration levels ranging from 5 to 60 mg/mL. Validation experiments revealed very good recovery rate of 9575.8% RSD, good linearity with r 2 values 40.999 within the established concentration range, excellent repeatability (0.52%), and reproducibility (1.67%) values (expressed as RSD), thus demonstrating the robustness and accuracy of the MEPS C8 /UPLC-photodiode array (PDA) method. The LOD of the method was 0.21 mg/mL, whereas the LOQ was 0.68 mg/mL. The validated methodology was applied to 30 commercial wines (24 red wines and six white wines) from different grape varieties, vintages, and regions. On the basis of the analytical validation, the MEPS C8 /UPLC-PDA methodology shows to be an improved, sensitive, and ultra-fast approach for determination of (E)-resveratrol in wines with high resolving power within 6 min.

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confidence: 99%

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

Gonçalves

Câmara

2011

J of Separation Science

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“…Microextraction in a packed syringe (MEPS) is a rather new technique used for the extraction and preconcentration of trace amounts of analytes into a solid support sorbent packed inside two frits in a syringe or microsyringe. [12][13][14] In this technique, sample preparation takes place on a packed bed of the sorbent. The sorbent is inserted in the barrel of a 1-mL insulin syringe as a plug with two polyethylene filters on both sides.…”

Section: Introductionmentioning

confidence: 99%

Determination of Three Organochlorine Pesticides in Aqueous Samples by Solid-Phase Extraction Based on Natural Nano Diatomite in Packed Syringe Coupled to Gas Chromatography-Mass Spectrometry

et al. 2017

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“…Most of the proposed analytical methods for determination of mepivacaine are based on separation techniques such as liquid chromatography, 3,5-9 gas chromatography [10][11][12][13][14] and electrophoresis. [15][16] Furthermore, an electrochemical method has been proposed for determination of mepivacaine.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence Determination of Local Anaesthetic Mepivacaine in Human Plasma and Pharmaceuticals

Mokhtari¹

2016

ACSi

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In this study, a new method has been developed for the simple determination of mepivacaine (carbocaine). The method is based on the enhancement effect of mepivacaine in the chemiluminescence (CL) reaction of tris(1,10 phenanthroline)ruthenium(II) with cerium(IV). A mechanism for the CL reaction has been proposed on the basis of UV-Vis, fluorescent and CL spectra. Under optimum conditions, the CL intensity was proportional to the concentration of the drug in solution over the range 0.45-226.25 μg mL -1 (R 2 = 0.9996). The LOD was 0.34 μg mL -1 (S/N = 3). LOD was about 10 times lower than the therapeutic concentration of mepivacaine. The percent of relative standard deviation for determination of 11 replicates at level of 9.05 μg mL -1 and 90.50 μg mL -1 of mepivacaine were 1.8 and 3.7%, respectively. The proposed method was applied successfully for the determination of mepivacaine in human plasma and injectable solutions.

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New trend in sample preparation: on-line microextraction in packed syringe for liquid and gas chromatography applications

Cited by 355 publications

References 14 publications

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

New method for determination of (E)‐resveratrol in wine based on microextraction using packed sorbent and ultra‐performance liquid chromatography

Determination of Three Organochlorine Pesticides in Aqueous Samples by Solid-Phase Extraction Based on Natural Nano Diatomite in Packed Syringe Coupled to Gas Chromatography-Mass Spectrometry

Chemiluminescence Determination of Local Anaesthetic Mepivacaine in Human Plasma and Pharmaceuticals

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