Determination of uremic solutes in biological fluids of chronic kidney disease patients by HPLC assay

Calaf, R; Cérini, Claire; Genovesio, Cecile; Verhaeghe, Pierre; Jourde-Chiche, Noémie; Bergé‐Lefranc, David; Gondouin, Bertrand; Dou, Laetitia; Morange, Sophie; Argilés, Àngel; Rathelot, Pascal; Dignat-George, Françoise; Brunet, Philippe; Charpiot, Philippe

doi:10.1016/j.jchromb.2011.06.014

Cited by 64 publications

(49 citation statements)

References 37 publications

(64 reference statements)

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“…Further studies, probably across populations, would help to understand the effects of these polymorphisms on plasma concentrations of 4b-hydroxycholesterol. Second, plasma concentrations of 3-INDS and 3-IAA were 10.9 6 9.4 and 2.6 6 1.4 mM, respectively, consistent with previous reports in patients with stages 3-5 chronic renal failure (Calaf et al, 2011). A significant negative correlation was observed between creatinine clearance and plasma concentration of 3-INDS, also consistent with previous reports (Huang et al, 2012).…”

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confidence: 91%

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Association of Plasma Concentration of 4β-Hydroxycholesterol with CYP3A5 Polymorphism and Plasma Concentration of Indoxyl Sulfate in Stable Kidney Transplant Recipients

et al. 2013

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Several studies have shown that renal failure decreases CYP3A activity and that uremic toxins may play a role via transcriptional or translational modifications of cytochrome P450 (P450) enzymes and direct inhibition of P450-mediated metabolism. In this study, we evaluated the relationship between CYP3A activity (using plasma concentration of 4b-hydroxycholesterol as a biomarker) and clinical characteristics including plasma concentrations of indoxyl sulfate (3-INDS) and indole-3-acetic acid (3-IAA) in stable kidney transplant recipients. Forty-five Japanese kidney transplant recipients who underwent transplantation more than 90 days prior to the study were included. Morning blood samples were collected and plasma concentrations of 4b-hydroxycholesterol, 3-INDS, and 3-IAA were measured. Plasma concentrations of 4b-hydroxycholesterol were 57.1 6 11.2, 42.1 6 11.8, and 34.5 6 7.3 ng/ml in recipients with CYP3A5*1/*1 (n = 5), *1/*3 (n = 15), and *3/*3 (n = 25) genotypes, respectively, with significant differences between three genotypes. A significant correlation was observed between plasma concentrations of 4b-hydroxycholesterol and 3-INDS but not 3-IAA. Multiple regression analysis identified the number of CYP3A5*3 alleles in genotype, plasma concentration of 3-INDS, and body weight as independent variables associated with plasma concentration of 4b-hydroxycholesterol. In conclusion, these results suggest that CYP3A5 polymorphism and plasma concentration of 3-INDS may account for the interindividual variability of CYP3A activity, and that plasma concentration of 3-INDS may partially explain the gap in CYP3A activity that cannot be explained by genetic contribution in patients with renal failure.

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confidence: 91%

“…Plasma concentrations of 3-INDS and 3-IAA were determined using a modification of the method previously reported (Calaf et al, 2011). To 100 ml of plasma in a polyethylene centrifuge tube, 300 ml of 16 mM p-ethylphenol in ethanol (internal standard) was added.…”

Section: Methodsmentioning

confidence: 99%

Association of Plasma Concentration of 4β-Hydroxycholesterol with CYP3A5 Polymorphism and Plasma Concentration of Indoxyl Sulfate in Stable Kidney Transplant Recipients

et al. 2013

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“…This increase in pore size enhances the molecular weight cut-off of the membranes to 100 kDa in vitro and to approximately 50-60 kDa in blood [29]. However, current dialysis membranes do not have pores of a uniform size that can translate with HCO membranes to the loss of essential proteins such as albumin.…”

Section: Discussionmentioning

confidence: 99%

Continuous Veno-Venous High Cut-Off Hemodialysis Compared to Continuous Veno-Venous Hemodiafiltration in Intensive Care Unit Acute Kidney Injury Patients

Balgobin¹,

Moréna²,

Brunot³

et al. 2018

Blood Purif

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Aims: High cut-off (HCO) continuous veno-venous hemodialysis (CVVHD) was compared to high-flux membrane (HFM) continuous veno-venous hemodiafiltration (CVVHDF) in intensive care unit (ICU) acute kidney injury (AKI) in terms of efficiency, hemodynamic tolerance, medium-sized molecules removal, albumin loss, and inflammatory system activation. Methods: In a prospective cross-over randomized study, 10 AKI patients underwent successively HCO (Ultraflux EmiC₂: β₂-microglobulin [β₂M] sieving coefficient [SC]: 0.9) CVVHD and HFM (Ultraflux AV1000S: β₂M SC: 0.65) CVVHDF. Results: Over the 20 sessions, hypotensive and febrile episodes, reduction rates of urea, creatinine, and β₂M were similar in both modalities. Though dialysis dose was higher with CVVHDF (36 ± 4 vs. 21 ± 6 mL/Kg/h), urea, creatinine, and β₂M instantaneous and plasmatic clearances did not differ except for urea at 12 h. Protein loss, superoxide anion production, cytokines, and growth factors variations were also comparable. Conclusion: HCO CVVHD is well tolerated and is as effective as HFM CVVHDF in clearance of solutes and removal of β₂M. It induces neither protein loss nor overproduction of superoxide anion. Video Journal Club “Cappuccino with Claudio Ronco” at http://www.karger.com/?doi=489082.

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“…A C C E P T E D ACCEPTED MANUSCRIPT 5 Several of these metabolites can be measured using existent methodologies, using gas chromatographic mass spectrometry [24], high performance liquid chromatography (HPLC) coupled to fluorescence and ultraviolet detection [21,[25][26][27] or mass spectrometry [28][29][30][31][32][33][34][35].…”

Section: A N U S C R I P Tmentioning

confidence: 99%

A liquid chromatography – tandem mass spectrometry method to measure a selected panel of uremic retention solutes derived from endogenous and colonic microbial metabolism

Loor

Poesen

Leger

et al. 2016

Analytica Chimica Acta

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Determination of uremic solutes in biological fluids of chronic kidney disease patients by HPLC assay

Cited by 64 publications

References 37 publications

Association of Plasma Concentration of 4β-Hydroxycholesterol with CYP3A5 Polymorphism and Plasma Concentration of Indoxyl Sulfate in Stable Kidney Transplant Recipients

Association of Plasma Concentration of 4β-Hydroxycholesterol with CYP3A5 Polymorphism and Plasma Concentration of Indoxyl Sulfate in Stable Kidney Transplant Recipients

Continuous Veno-Venous High Cut-Off Hemodialysis Compared to Continuous Veno-Venous Hemodiafiltration in Intensive Care Unit Acute Kidney Injury Patients

A liquid chromatography – tandem mass spectrometry method to measure a selected panel of uremic retention solutes derived from endogenous and colonic microbial metabolism

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