A liquid chromatography – tandem mass spectrometry method to measure a selected panel of uremic retention solutes derived from endogenous and colonic microbial metabolism

Loor, Henriëtte de; Poesen, Ruben; Leger, Wout De; Dehaen, Wim; Augustijns, Patrick; Evenepoel, Pieter; Meijers, Björn

doi:10.1016/j.aca.2016.06.057

Cited by 43 publications

(40 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hence, this unknown substrate might be closely similar molecular formula and physical property of IndG. de Loor et al [7] did reported no adjacent peaks close to IndG in serum samples; thus, it is likely that it may be a fact observed only in urine. Nonetheless, the peak of IndG was hardly detected in 16-fold diluents of mixed urine (Fig.…”

Section: Peak Specificitymentioning

confidence: 94%

“…The analytical condition was adjusted as previously described [7], with some modifications. Briefly, an UHPLC device equipped with a binary solvent manager, an autosampler, a column heater, and tandem mass spectrometry (Acquity TQD System; Waters) was used to detect the conjugated forms of IPC.…”

Section: Instrumentsmentioning

confidence: 99%

“…For example, the conjugated forms of IPC were observed to accelerate CKD [6]. Among the conjugated forms of IPC, p-cresyl sulfate (PCS) and indoxyl sulfate (IndS) are representative and have been repeatedly associated with overall mortality, cardiovascular disease and progression of CKD [7]. Particularly, the progression of CKD caused by IndS is believed to be induced by gene transcription associated with renal fibrosis [6].…”

Section: Introductionmentioning

confidence: 99%

“…Rapid and accurate detection of the conjugated forms of IPC in urine and blood is likely to be useful for establishing the occurrence or degree of progression of CKD. Several simultaneous methods by liquid chromatography-tandem mass spectrometry (LC-MS/MS) of quantification of the conjugated forms of IPC in blood serum were reported [7,12]. However, those methods were designed for the analysis of human serum, and their detection sensitivity of the conjugated forms of IPC in human urine is still limited.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, we modified the previously described determination methods [7] to analyze human urine samples by ultra high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). In addition, we validated the newly-modified methods.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Simultaneous Analysis of Glucuronyl- and Sulpho-Conjugates of Intestinal Putrefactive Compounds in Human Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

et al. 2019

View full text Add to dashboard Cite

Glucuronyl-and sulpho-conjugates of intestinal putrefactive compounds (IPC) are considered kidney disease-associated toxic agents. Recently, several simultaneous methods of detection by liquid chromatography tandem mass spectrometry (LC-MS/MS) of the conjugated forms of glucuronyl-and sulpho-conjugates in blood serum were reported. To analyze these conjugated forms in urine, we modified and validated the LC-MS/MS detection methods. The range of the calibration curves were determined using phenyl glucuronide (PhG; 1.56-25.0 μM), indoxyl glucuronide (IndG; 1.25-20.0 μM), p-cresyl glucuronide (PCG; 0.625-10.0 μM), phenyl sulfate (PhS; 3.13-50.0 μM), indoxyl sulfate (IndS; 7.81-250 μM) and p-cresyl sulfate (PCS; 1.56-25.0 μM). Urine was diluted 2-4 fold to determine PhG, IndG and PCG concentrations, and 4-16 fold to determine PhS, IndS and PCS concentrations. We validated the newly-modified detection methods by a recovery test, and intra-day and inter-day repeatability. We analyzed six clinical urine samples, PCS and IndS, major toxic agents, which were mostly detected. Creatinine-corrected values of PCS and IndS were high in six conjugates. In summary, in the present work, the concentrations of six urinary conjugated forms of IPC were successfully measured by LC-MS/MS.

show abstract

Section: Peak Specificitymentioning

confidence: 94%

Section: Instrumentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Simultaneous Analysis of Glucuronyl- and Sulpho-Conjugates of Intestinal Putrefactive Compounds in Human Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

et al. 2019

View full text Add to dashboard Cite

show abstract

The Dietary Fiber Inulin Slows Progression of Chronic Kidney Disease–Mineral Bone Disorder (CKD‐MBD) in a Rat Model of CKD

Biruete,

Chen,

Metzger

et al. 2023

JBMR Plus

Self Cite

View full text Add to dashboard Cite

Chronic kidney disease (CKD)–mineral bone disorder (CKD‐MBD) leads to fractures and cardiovascular disease. Observational studies suggest beneficial effects of dietary fiber on both bone and cardiovascular outcomes, but the effect of fiber on CKD‐MBD is unknown. To determine the effect of fiber on CKD‐MBD, we fed the Cy/+ rat with progressive CKD a casein‐based diet of 0.7% phosphate with 10% inulin (fermentable fiber) or cellulose (non‐fermentable fiber) from 22 weeks to either 30 or 32 weeks of age (~30% and ~15% of normal kidney function; CKD 4 and 5). We assessed CKD‐MBD end points of biochemistry, bone quantity and quality, cardiovascular health, and cecal microbiota and serum gut‐derived uremic toxins. Results were analyzed by two‐way analysis of variance (ANOVA) to evaluate the main effects of CKD stage and inulin, and their interaction. The results showed that in CKD animals, inulin did not alter kidney function but reduced the increase from stage 4 to 5 in serum levels of phosphate and parathyroid hormone, but not fibroblast growth factor‐23 (FGF23). Bone turnover and cortical bone parameters were similarly improved but mechanical properties were not altered. Inulin slowed progression of aorta and cardiac calcification, left ventricular mass index, and fibrosis. To understand the mechanism, we assessed intestinal microbiota and found changes in alpha and beta diversity and significant changes in several taxa with inulin, together with a reduction in circulating gut derived uremic toxins such as indoxyl sulfate and short‐chain fatty acids. In conclusion, the addition of the fermentable fiber inulin to the diet of CKD rats led to a slowed progression of CKD‐MBD without affecting kidney function, likely mediated by changes in the gut microbiota composition and lowered gut‐derived uremic toxins. © 2023 The Authors. JBMR Plus published by Wiley Periodicals LLC. on behalf of American Society for Bone and Mineral Research.

show abstract

Rapid and sustainable HPLC method for the determination of uremic toxins in human plasma samples

et al. 2022

View full text Add to dashboard Cite

A liquid chromatography – tandem mass spectrometry method to measure a selected panel of uremic retention solutes derived from endogenous and colonic microbial metabolism

Cited by 43 publications

References 43 publications

Simultaneous Analysis of Glucuronyl- and Sulpho-Conjugates of Intestinal Putrefactive Compounds in Human Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

Simultaneous Analysis of Glucuronyl- and Sulpho-Conjugates of Intestinal Putrefactive Compounds in Human Urine by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

The Dietary Fiber Inulin Slows Progression of Chronic Kidney Disease–Mineral Bone Disorder (CKD‐MBD) in a Rat Model of CKD

Rapid and sustainable HPLC method for the determination of uremic toxins in human plasma samples

Contact Info

Product

Resources

About