Determination of tyrosine through a FIA-direct chemiluminescence procedure

Graphene quantum dots (GQD) could significantly enhance the chemiluminescence (CL) reaction of Ce(IV) with NaHSO3 through energy and electron transfer. The chemiluminescence resonance energy transfer (CRET) occurred between SO2*/1O2 and GQD, and radiative electron–hole annihilation came from the combining of the hole‐injected GQD (GQD•+) and electron‐injected GQD (GQD•–). Tyrosine (Tyr) is easily oxidized by OH• and Ce(IV), which results in the decrease of OH• and Ce(IV), and the CL intensity of the GQD–Ce(IV)–NaHSO3 system is inhibited by Tyr. In this way, the GQD‐based CL as a sensitive and selective method was applied for the detection of Tyr in the range 0.02–8.00 μM with the detection limit of 4.0 nM. The method was applied to the determination of Tyr in food samples with satisfactory results.

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“…Table S2 compares the features of the proposed method with those of other methods for the determination of Tyr. [33][34][35][36][37][38][39][40]…”

Section: Analytical Performancementioning

confidence: 99%

Graphene quantum dot‐enhanced chemiluminescence through energy and electron transfer for the sensitive detection of tyrosine

Pang

Han

et al. 2018

J Chinese Chemical Soc

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“…Alternatively, chemiluminescence (CL)-based analytical methods are highly interesting owing to their high sensitivity. Till now, many methods have been reported for CL determination of l -tyrosine. ,− However, these methods relied on either the quenching effect of l -tyrosine on a luminol-based CL system or the nonselective reaction with oxidizing agents, which limited their selectivity and sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Development of a Selective Assay of Tyrosine and Its Producing and Metabolizing Enzymes Utilizing Pulse-UV Irradiation-Induced Chemiluminescence

et al. 2022

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A new pulse UV irradiation-induced chemiluminescence (CL) determination method was developed for L-tyrosine using the luminol derivative L-012. The proposed method depends on the formation of reactive oxygen species (ROS) upon pulse UV irradiation of L-tyrosine; then, these ROS react with L-012 producing strong CL. The proposed method showed excellent sensitivity and ultraselectivity toward L-tyrosine. The mechanism of the developed CL method was studied using ROS scavengers, HPLC, and mass spectrometry. The method was linear for L-tyrosine in the range of 0.03−50 μM. Minor changes in the L-tyrosine structure, including hydroxylation, dehydroxylation, phosphorylation, or decarboxylation, were found to lead to a strong decrease in CL. Using the excellent selectivity of the proposed method for L-tyrosine, we have developed a CL assay for measuring alkaline phosphatase activity in the range of 0.02−15 U/L with the limit of detection (LOD) of 4 mU/L using the nonchemiluminescent Ophospho-L-tyrosine as a substrate. Furthermore, the CL reaction was applied for tyrosinase activity assay as this enzyme can convert L-tyrosine to the nonchemiluminescent L-dopa. The decrease in CL is correlated with the tyrosinase activity in the range of 0.025− 0.75 U/mL with an LOD of 1.5 mU/mL. Moreover, the tyrosinase activity assay was successfully applied for the determination of IC 50 of the tyrosinase inhibitors kojic acid and benzoic acid. Therefore, our novel pulse UV irradiation CL method for the determination of L-tyrosine was not only suitable for the determination of this vital amino acid but also extended to the successful determination of its producing and metabolizing enzymes and their inhibitors.

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“…Though analytical methods like chro matography, fluorescence, mass spectrometry and other techniques are available for the detection of Tyr [19][20][21][22], DA [23][24][25][26][27][28][29][30][31] and AA [32][33][34][35][36][37][38] in biological fluids, they are useful to certain extent due to their own limitations. Owing to fast response, accuracy and portability, the electrochemical methods using electrochemical sensors [39] to analy ze the DA, AA and Tyr coexisting in biological flu id [40][41][42][43] are very promising in accurate quantification of these bio-molecu les.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous detection of dopamine, tyrosine and ascorbic acid using NiO/graphene modified graphite electrode

2020

Biointerface Res Appl Chem

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In this work, an electrochemical sensor is fabricated by decorating the surface of graphite electrode with NiO/graphene (NGMG) nanoparticles and employed for the detection of dopamine (DA), tyrosine (Tyr) and ascorbic acid (AA). The structure and morphology of prepared NiO nanoparticles are examined by XRD,SEM, FTIR and Raman techniques. The electrochemical properties have been investigated by cyclic voltammetry (CV), differential pulse voltammetry (DPV) and chronoamperommety. The modified electrode is prepared by a simple drop casting method. The electrode shows good electro catalyticactivity towards oxidation of DA, Tyr and AA. It successfully separates the oxidation current signals of AA, DA and Tyr into clearly visible three distinct oxidation peaks compared to a single, overlapped oxidative peak on bare graphite electrode. The peak potential difference between AA-DA, DA-Tyr and AA-Tyr is 228 mV, 303 mV and 565 mV respectively in cyclic voltammetry (CV) studies and the corresponding peak potential separations are 243 mV, 318 mV and 561 mV respectively in differential pulse voltammetry (DPV). It is found that oxidation mechanism of DA, AA and Tyr on NGMG are different owing to a different type of interaction of the modified layer with the bio-analytes. The modified electrode, NGMG has high selectivity and sensitivity in addition to other factors like low cost, convenient and a hassle free electrochemical method for simultaneous determination of DA, AA and Tyr in their ternary mixture.

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Determination of tyrosine through a FIA-direct chemiluminescence procedure

Cited by 51 publications

References 20 publications

Graphene quantum dot‐enhanced chemiluminescence through energy and electron transfer for the sensitive detection of tyrosine

Graphene quantum dot‐enhanced chemiluminescence through energy and electron transfer for the sensitive detection of tyrosine

Development of a Selective Assay of Tyrosine and Its Producing and Metabolizing Enzymes Utilizing Pulse-UV Irradiation-Induced Chemiluminescence

Simultaneous detection of dopamine, tyrosine and ascorbic acid using NiO/graphene modified graphite electrode

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