Determination of tryptamine derivatives in hallucinogenic mushrooms using high-performance liquid chromatography with photodiode array detection

Borner, Stefan; Brenneisen, Rudolf

doi:10.1016/s0021-9673(01)81831-8

Cited by 38 publications

(14 citation statements)

References 13 publications

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“…Another disadvantage of using these columns is that the different polarity of psilocybin and psilocin makes simultaneous analysis difficult. The problem may be solved, at least in part, by using a mobile phase gradient (Borner and Brenneisen, 1987) or by using two different solvent systems for these two compounds (Kysilka and Wurst 1990). In none of the published methods using C18 columns under isocratic conditions was it confirmed whether these systems were able to separate psilocibin and its demethylated analogue, baeocystin Wurst et al 1984;Semerdžieva et al, 1986;Gartz, 1987aGartz, , 1989bKysilka et al, 1985;Kysilka and Wurst 1989;Gartz & Müller, 1989;Kysilka & Wurst, 1990;Wurst et al 1992).…”

Section: Quantitative Determination Of Psilocybin and Psilocin In Musmentioning

confidence: 99%

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Andersson¹,

Gry²,

Kristinsson³

2009

TemaNord

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Section: Quantitative Determination Of Psilocybin and Psilocin In Musmentioning

confidence: 99%

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Andersson¹,

Gry²,

Kristinsson³

2009

TemaNord

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“…DMT in ayahuasca (a South American psychotropic beverage) and in human plasma after dosing with ayahuasca was quantified by TLC and HPLC-UV detector (UVD) [4] and by GC-nitrogen-phosphorus detector (NPD) [5,6]. Tryptamine derivatives in plant and mushroom extracts were identified and quantified by RP-HPLC-UVD [7], RP-HPLC-DAD [8,9], RP-HPLC-UVD combined with amperometric detection [10,11], or ion-exchange chro- Figure 1. Structures of tryptamine derivatives quantified in this study: (1) tryptamine, (2) 5-methyltryptamine, (3) DET, (4) 5-MeO-DMT, (5) DMT, (6) 6-methoxytryptamine, (7) serotonin, (8) bufotenine, (9) 5-MeO-DIPT, (10) acridine (i.st.…”

Section: Introductionmentioning

confidence: 99%

“…Tryptamine derivatives in plant and mushroom extracts were identified and quantified by RP-HPLC-UVD [7], RP-HPLC-DAD [8,9], RP-HPLC-UVD combined with amperometric detection [10,11], or ion-exchange chro- Figure 1. Structures of tryptamine derivatives quantified in this study: (1) tryptamine, (2) 5-methyltryptamine, (3) DET, (4) 5-MeO-DMT, (5) DMT, (6) 6-methoxytryptamine, (7) serotonin, (8) bufotenine, (9) 5-MeO-DIPT, (10) acridine (i.st. ), (11) a-ethyltryptamine (a-ET), (12) a-methyltryptamine (a-MT), (13) NMT.…”

Section: Introductionmentioning

confidence: 99%

Determination of tryptamine derivatives in illicit synthetic drugs by capillary electrophoresis and ultraviolet laser-induced fluorescence detection

et al. 2005

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A method based on separation by capillary electrophoresis combined with UV-laser-induced fluorescence detection (Lambdaex = 266 nm) was developed for the determination of nine tryptamine derivatives of forensic interest and potential matrix constituents. The composition of the separation electrolyte was optimized with respect to the resolution of solutes of interest and to the sensitivity of fluorescence detection. Native alpha-cyclodextrin was employed as a complex forming modifier of the electrophoretic separation and fluorescence-enhancing agent. With the help of a stacking procedure, limits of detection of 0.1-6 microg/L for all analytes were obtained. The repeatability for the peak area (at a concentration of the analyte about 100 times the LOD) was less than 2.3% RSD. A second HPLC method was developed, and its analytical parameters were evaluated for an estimation of the accuracy of the CE-LIF method and for method comparison. The results of the determination of tryptamine derivatives in the samples of forensic interest obtained with the two independent methods are in good agreement.

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“…Several techniques have been employed to determine psilocin and psilocybin in hallucinogenic mushrooms for forensic analysis, with high-performance liquid chromatography (HPLC) being by far the most widely used analytical technique. Detection modes include ultraviolet (UV) (1,2,(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18), fluorescence (2,12,13,17,19,20), electrochemical (12), voltametric detection, (6,9), and mass spectrometry (2,5,13,18,20). Other techniques for the determination of psilocin and psilocybin in mushroom samples also include gas chromatography-mass spectrometry (21)(22)(23), capillary electrophoresis (3), infrared spectroscopy (8,23), thin layer chromatography (2,15,18,21,24), gas chromatography (6,25), ion mobility spectrometry (22), and flow injection analysis (26).…”

mentioning

confidence: 99%

The Determination of Psilocin and Psilocybin in Hallucinogenic Mushrooms by HPLC Utilizing a Dual Reagent Acidic Potassium Permanganate and Tris(2,2′‐bipyridyl)ruthenium(II) Chemiluminescence Detection System

Anastos

Lewis

Barnett

et al. 2006

Journal of Forensic Sciences

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This paper describes a procedure for the determination of psilocin and psilocybin in mushroom extracts using high-performance liquid chromatography with postcolumn chemiluminescence detection. A number of extraction methods for psilocin and psilocybin in hallucinogenic mushrooms were investigated, with a simple methanolic extraction being found to be most effective. Psilocin and psilocybin were extracted from a variety of hallucinogenic mushrooms using methanol. The analytes were separated on a C12 column using a (95:5% v/v) methanol:10 mM ammonium formate, pH 3.5 mobile phase with a run time of 5 min. Detection was realized through a dual reagent chemiluminescence detection system of acidic potassium permanganate and tris(2,2'-bipyridyl)ruthenium(II). The chemiluminescence detection system gave improved detectability when compared with UV absorption at 269 nm, with detection limits of 1.2 x 10(-8) and 3.5 x 10(-9) mol/L being obtained for psilocin and psilocybin, respectively. The procedure was applied to the determination of psilocin and psilocybin in three Australian species of hallucinogenic mushroom.

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Determination of tryptamine derivatives in hallucinogenic mushrooms using high-performance liquid chromatography with photodiode array detection

Cited by 38 publications

References 13 publications

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Occurrence and use of hallucinogenic mushrooms containing psilocybin alkaloids

Determination of tryptamine derivatives in illicit synthetic drugs by capillary electrophoresis and ultraviolet laser-induced fluorescence detection

The Determination of Psilocin and Psilocybin in Hallucinogenic Mushrooms by HPLC Utilizing a Dual Reagent Acidic Potassium Permanganate and Tris(2,2′‐bipyridyl)ruthenium(II) Chemiluminescence Detection System

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