Determination of triiodothyronine concentration in human serum

Sterling, Kenneth; Bellabarba, Diégo; Newman, Edward S.; Brenner, Milton A.

doi:10.1172/jci106072

Cited by 171 publications

(85 citation statements)

References 34 publications

(58 reference statements)

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“…Serum T3 concentrations varied between 269 and 680 ng/100 ml and averaged 452 ng/100 ml. The T3 values, therefore, were mainly above the normal range (170-270 ng/100 ml) and normal mean +SD (220 ±27 ng/100 ml) (8). The mean value of the T4: T3 ratio in the sera of patients receiving oral L-T4 was 34: 1 (Table I) Fig.…”

Section: Methodological Control Experimentsmentioning

confidence: 88%

“…Extensive quantitative analyses were carried out on all lots of stable and radioactive T4 administered, since it was obviously essential to assess T3 contamination in the administered T4. The approach to this problem was a modification of the analytic procedure previously developed (8), including the improved paper chromatographic separation of T3 and T4.…”

Section: Methodological Control Experimentsmentioning

confidence: 99%

“…Analyses for the concentration of stable T3 in serum were carried out by the method of Sterling, Bellabarba, Newman, and Brenner (8). Briefly, this procedure consists of (a) removal of the thyroid hormones from serum with cation exchange resin columns; (b) separation of T3 from T4 by descending paper chromatography with a hexane-tertiary amyl alcohol-ammonia solvent system, and (c) measurement of the eluted T3 by the binding displacement technique.…”

Section: Laboratory Techniquesmentioning

confidence: 99%

See 2 more Smart Citations

Conversion of Thyroxine (T4) to triiodothyronine (T3) in athyreotic human subjects

Braverman¹,

Ingbar²,

Sterling³

1970

J. Clin. Invest.

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462

167

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A B S T R A C T Studies of the possibility that thyroxine (T4) is converted to 3,5,3'-triiodo-L-thyronine (T3) in the extrathyroidal tissues in man have been conducted in 13 patients, all but two of whom were athyreotic or hypothyroid, and all of whom were receiving at least physiological replacement doses of synthetic sodium-Lthyroxine.T3 was found in the sera of all patients, in concentrations ranging between 243 and 680 ng/100 ml (normal range 170-270 ng/100 ml). These concentrations were far in excess of those which would have been expected on the basis of the T3 contamination of the administered T4, as measured by the same technique employed in the analysis of serum. When oral medication was enriched with 'I-labeled T4 for 8 or more days, labeled T3 and tetraiodothyroacetic acid (Tetrac or TA4) were found in the serum to the extent of approximately 2-5% of total radioactivity, as assessed by unidimensional paper chromatography. The same results were obtained with a specially purified lot of radioactive T4 containing less than 0.1% T3 as a contaminant. The identities of the 'I-labeled T3 and TA4 were verified by two-dimensional chromatography as well as by specific patterns of binding in serum. The labeled T3 isolated was bound by albumin and by T4-binding globulin (TBG), but not by T4-binding prealbumin (TBPA); in contrast the labeled TA4 was bound by albumin and TBPA, but not by TBG.To exclude the possibility that the conversion of T4 to T3 was a peculiarity of the oral route of administra- There is no doubt whatsoever that at least a portion of the T3 in the blood results from direct secretion by the thyroid gland, T3 having been found in the thyroid venous effluent, and in concentrations substantially higher than in the concurrently sampled arterial blood (4, 5). What has remained uncertain, however, is what fraction, if any, of the T3 in the blood arises from the

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Section: Methodological Control Experimentsmentioning

confidence: 88%

Section: Methodological Control Experimentsmentioning

confidence: 99%

Section: Laboratory Techniquesmentioning

confidence: 99%

See 1 more Smart Citation

Conversion of Thyroxine (T4) to triiodothyronine (T3) in athyreotic human subjects

Braverman¹,

Ingbar²,

Sterling³

1970

J. Clin. Invest.

Self Cite

462

167

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show abstract

“…The recent availability of techniques for measuring triiodothyronine (Ts)1 in human serum has provoked new interest in this thyroid hormone (1)(2)(3). It is speculated that Ts may provide as much as 50-60% of the body's thyroid hormone requirements (2).…”

Section: Introductionmentioning

confidence: 99%

Salicylate-induced increases in free triiodothyronine in human serum

Larsen¹

1972

J. Clin. Invest.

164

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“…The subsequent procedure for calculating the uptake kinetics is similar to that in [ 11. Free Ts concentrations at the different albumin concentrations used were estimated with equilibrium dialysis [4]. The range of the free Ts concentrations ([FTs]) was 1.3 nM to 76 FM.…”

mentioning

confidence: 99%