Determination of trichothecenes in wheat by capillary gas chromatography with flame ionisation detection

Schothorst, R.C.; Jekel, A. A.

doi:10.1016/s0308-8146(00)00321-6

Cited by 61 publications

(26 citation statements)

References 6 publications

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“…The LOQ for DON is below the maximum permitted limit in cereals set by legislation (European Commission, 2006a), and to the author's knowledge, the LODs obtained for the trichothecenes in barley are lower or similar to others described for cereals in the reference literature (Eskola, Parikka and Rizzo, 2001, Schothorst and Jekel, 2001, Lattanzio, Solfrizzo and Visconti, 2008.…”

Section: Discusionmentioning

confidence: 45%

Simultaneous determination of type-A and type-B trichothecenes in barley samples by GC–MS

2011

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Section: Discusionmentioning

confidence: 45%

Simultaneous determination of type-A and type-B trichothecenes in barley samples by GC–MS

2011

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“…Gas chromatography methods have been based on flame ionisation detection (FID) Jekel, 2001 and2003), electroncapture (ECD) (Molto et al, 2000) or mass spectrometric (MS) detection (Schollenberger et al, 2005). Mostly a derivatization step of the hydroxyl groups is included in order to increase volatility and sensitivity.…”

Section: Methodsmentioning

confidence: 99%

Scientific information on mycotoxins and natural plant toxicants

Battilani

Costa

Dossena

et al. 2009

EFSA Supporting Publications

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“…Various solvents or mixtures of solvents are recommended by numerous authors as optimal to efficiently extract trichothecenes from samples of grain-based food and feed (ValleAlgarra et al 2005;Stecher et al 2007;Cano-Sancho et al 2011;Schothorst and Jekel 2001). The mixture of acetonitrile/ water (84:16) is a popular choice and was used during the study.…”

Section: Clean-upmentioning

confidence: 99%

“…Various chromatographic methods commonly used for quantitative determination of trichothecenes in cereal-based products include gas chromatography with electron capture, flame ionisation or mass spectrometry (MS) detectors (Nielsen and Thrane 2001;Schothorst and Jekel 2001;Ibáñez-Vea et al 2011;Valle-Algarra et al 2011) and highperformance liquid chromatography (HPLC) with ultraviolet, diode array, fluorescence or MS detectors (Jiménez et al 2000;Dall'Asta et al 2004;Biancardi et al 2005;Santini et al 2009;Monaci et al 2011). Liquid chromatography (LC)-MS spectrometers with triple quadrupole mass analysers have been most frequently used in labs dealing with trichothecene analyses.…”

Section: Introductionmentioning

confidence: 99%

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

et al. 2013

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The aim of this work was to evaluate the usefulness of the ion trap mass spectrometry coupled to high-performance liquid chromatography for simultaneous determination of selected trichothecenes (nivalenol, deoxynivalenol, fusarenon-X, neosolaniol, 3-acetyl-deoxynivalenol, diacetoxyscirpenol, HT-2 and T-2 toxins) in grain products. These compounds were extracted from the grain products and then cleaned up with the developed, simple and robust procedure using some mixture of neutral alumina, charcoal and diatomaceous earth. Method recovery was 88-125 % depending on combination of the analysed mycotoxins, sample matrix and the fortification level. Method precision expressed by relative standard deviation ranged from 2.6 to 27.4 %. The concentrations of the selected trichothecenes have been determined in 94 samples of cerealbased products. Maize-based next to wheat-based products were the most contaminated with deoxynivalenol, neosolaniol, 3-acetyl-deoxynivalenol, diacetoxyscirpenol and HT-2 toxin. In 83 % of wheat-based products, deoxynivalenol was determined at the average level of 249 μg kg −1 . The highest concentration of deoxynivalenol-2,026 μg kg −1 (476±471 μg kg −1 on the average)-was found in the maize-based product. Other mycotoxins were found much less frequently: 3-acetyl-deoxynivalenol in only one sample at the concentration of 59 μg kg −1 , neosolaniol, HT-2 toxin and diacetoxyscirpenol in a few samples on average concentrations close to respective limits of quantification.

show abstract

Determination of trichothecenes in wheat by capillary gas chromatography with flame ionisation detection

Cited by 61 publications

References 6 publications

Simultaneous determination of type-A and type-B trichothecenes in barley samples by GC–MS

Simultaneous determination of type-A and type-B trichothecenes in barley samples by GC–MS

Scientific information on mycotoxins and natural plant toxicants

An LC-IT-MS/MS-Based Method to Determine Trichothecenes in Grain Products

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