Determination of trace sulfides in turbid waters by gas dialysis/ion chromatography

Goodwin, Lorne R.; Francom, Donna M.; Urso, Alessandro; Dieken, Fred P.

doi:10.1021/ac00154a006

Cited by 28 publications

(5 citation statements)

References 24 publications

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“…For each experiment (n = 6), 30 to 50 released swarmers from about 50 colonies kept in oxic seawater (24 °C ± 1 °C, salinity 38 ± 1) were transferred into the central plastic cube (8 × 8 × 7 cm) of the preference chamber filled with oxic seawater. On each side of the cube, one vial was mounted and sealed with a dialysis polyethylene membrane (2.6 cm in diameter) permeable for small molecules to allow diffusion into the central chamber 81 (Supplementary Fig. S1).…”

Section: Methodsmentioning

confidence: 99%

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Bright

Espada-Hinojosa

Volland

et al. 2019

Sci Rep

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Evolutionary theory predicts potential shifts between cooperative and uncooperative behaviour under fluctuating environmental conditions. This leads to unstable benefits to the partners and restricts the evolution of dependence. High dependence is usually found in those hosts in which vertically transmitted symbionts provide nutrients reliably. Here we study host dependence in the marine, giant colonial ciliate Zoothamnium niveum and its vertically transmitted, nutritional, thiotrophic symbiont from an unstable environment of degrading wood. Previously, we have shown that sulphidic conditions lead to high host fitness and oxic conditions to low fitness, but the fate of the symbiont has not been studied. We combine several experimental approaches to provide evidence for a sulphide-tolerant host with striking polyphenism involving two discrete morphs, a symbiotic and an aposymbiotic one. The two differ significantly in colony growth form and fitness. This polyphenism is triggered by chemical conditions and elicited by the symbiont’s presence on the dispersing swarmer. We provide evidence of a single aposymbiotic morph found in nature. We propose that despite a high fitness loss when aposymbiotic, the ciliate has retained a facultative life style and may use the option to live without its symbiont to overcome spatial and temporal shortage of sulphide in nature.

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Section: Methodsmentioning

confidence: 99%

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Bright

Espada-Hinojosa

Volland

et al. 2019

Sci Rep

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“…Samples were analyzed according to Goodwin's method (Goodwin et al 1988) as adapted to biological materials (Warenycia et al 1989a) by a continuous flow procedure using a gas dialysis membrane unit (Technicon; 157-B-129; path length 15 cm). Strong acid (6.0 M HCI) was added (0.1 ml/min) to the alkaline sample stream (1.2 ml/min) to release H2S from the brain homogenate.…”

Section: Sulfide Analysismentioning

confidence: 99%

Dithiothreitol liberates non-acid labile sulfide from brain tissue of H2S-poisoned animals

et al. 1990

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Abstract. Acid-labile sulfide measured by conventional gas dialysis and ion chromatography with electrochemical detection accounts for only a proportion of the total sulfide present in brain tissue after poisoning with NariS, an H2S precursor. Dithiothreitol (DTT) displaced additional measurable sulfide not detectable by the conventional techniques from NariS-poisoned brain tissue. Sulfide liberation by DTT was dose-dependent and maximal at higher DTI" concentration (10 and 30 mM) and was thought to represent non-acid labile sulfide. Dithiothreitol was also found to be significantly protective against H2S poisoning. Furthermore, in vitro inhibition by sulfide of monoamine oxidase (MAO) was reversed by DTT, thus suggesting a molecular mechanism consistent with known persulfide chemistry. Persulfide formation may thus underlie some aspects of hydrogen sulfide neurotoxicity. The rational development of antidotes for use in H2S poisoning may thus have to be centered on strategies concentrating on known thiol, disulfide and persulfide chemistry.

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“…In general, the use of chromatography in parallel with tandem MS/MS is attractive due to its low nM detection limits, application to various biological thiols, and use in biological media. In addition, chromatographic approaches for sulfide detection using flow gas dialysis coupled with electrochemical quantification by ion chromatography have been reported. , This method reports good recovery of sample (95–99%) and has been used to measure post-mortem sulfide levels in rat and human brain tissue, although the required gas dialysis pretreatment step increases the complexity of this approach.…”

Section: Quantification Approachesmentioning

confidence: 99%

Advances and Opportunities in H₂S Measurement in Chemical Biology

Smith,

Pluth

2023

JACS Au

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Hydrogen sulfide (H2S) is an important biological mediator across all kingdoms of life and plays intertwined roles in various disciplines, ranging from geochemical cycles to industrial processes. A common need across these broad disciplines is the ability to detect and measure H2S in complex sample environments. This Perspective focuses on key advances and opportunities for H2S detection and quantification that are relevant to chemical biology. Specifically, we focus on methods for H2S detection and quantification most commonly used in biological samples, including activity-based H2S probes, the methylene blue assay, the monobromobimane assay, and H2S-sensitive electrode measurements. Our goal is to help simplify what at first may seem to be an overwhelming array of detection and measurement choices, to articulate the strengths and limitations of individual techniques, and to highlight key unmet needs and opportunities in the field.

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Determination of trace sulfides in turbid waters by gas dialysis/ion chromatography

Cited by 28 publications

References 24 publications

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Dithiothreitol liberates non-acid labile sulfide from brain tissue of H2S-poisoned animals

Advances and Opportunities in H₂S Measurement in Chemical Biology

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Determination of trace sulfides in turbid waters by gas dialysis/ion chromatography

Cited by 28 publications

References 24 publications

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Thiotrophic bacterial symbiont induces polyphenism in giant ciliate host Zoothamnium niveum

Dithiothreitol liberates non-acid labile sulfide from brain tissue of H2S-poisoned animals

Advances and Opportunities in H2S Measurement in Chemical Biology

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Advances and Opportunities in H₂S Measurement in Chemical Biology