Dithiothreitol liberates non-acid labile sulfide from brain tissue of H2S-poisoned animals

Abstract

Abstract. Acid-labile sulfide measured by conventional gas dialysis and ion chromatography with electrochemical detection accounts for only a proportion of the total sulfide present in brain tissue after poisoning with NariS, an H2S precursor. Dithiothreitol (DTT) displaced additional measurable sulfide not detectable by the conventional techniques from NariS-poisoned brain tissue. Sulfide liberation by DTT was dose-dependent and maximal at higher DTI" concentration (10 and 30 mM) and was thought to represent non-acid labile sulfide. Dithiothreitol was also found to be significantly protective against H2S poisoning. Furthermore, in vitro inhibition by sulfide of monoamine oxidase (MAO) was reversed by DTT, thus suggesting a molecular mechanism consistent with known persulfide chemistry. Persulfide formation may thus underlie some aspects of hydrogen sulfide neurotoxicity. The rational development of antidotes for use in H2S poisoning may thus have to be centered on strategies concentrating on known thiol, disulfide and persulfide chemistry.

Hydrogen Sulfide Inhibits Rotenone-Induced Apoptosis via Preservation of Mitochondrial Function

“…This triggering effect of H 2 S may be similar to the mechanism for the cardioprotection conferred by H 2 S preconditioning (Pan et al, 2008). It is interesting that it was also reported that H 2 S can be rapidly absorbed in a kind of sulfur store in a form of bound sulfane sulfur and then gradually released upon stimulation (Warenycia et al, 1990;Ogasawara et al, 1993). Therefore, this may also explain the persistent protective effects of H 2 S despite its short half-life.…”

Hydrogen Sulfide Attenuates Neuronal Injury Induced by Vascular Dementia Via Inhibiting Apoptosis in Rats

“…In the present study, 14 lmol/kg of NaHS was intraperitoneally administered and it was confirmed that the dose of NaHS conferred a protective effect on neuronal injury induced by VD. Interestingly, it was also reported that H 2 S can be rapidly absorbed in a kind of sulfur store in a form of bound sulfane sulfur and then gradually released upon stimulation [46,47]. Therefore, a low concentration of H 2 S may be offered continually into the body and this may also explain the persistent protective effects of H 2 S despite its short half-life.…”

Measurement and biological significance of the volatile sulfur compounds hydrogen sulfide, methanethiol and dimethyl sulfide in various biological matrices

“…This method is performed at a pH < 1 and therefore also measures ALS. Only a limited amount of reports [29,46,48,[61][62][63] clearly distinguish between the analysis of ALS and DLS.…”