Determination of Trace Elements in Human Tissue. I. Cd, Fe, Zn, Mg, and Ca

Marks, Gayle E.; Moore, Carl R.; Kanabrocki, Eugene L.; Oester, Y. T.; Kaplan, Ervin

doi:10.1366/000370272774351769

Cited by 22 publications

(17 citation statements)

References 6 publications

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“…Hepatic iron levels were determined after liquid digestion of dry tissue by atomic absorption spectrophotometry (Perkin-Elmer -5.100, Norwalk, CT, USA) (16).…”

mentioning

confidence: 99%

Dietary cellulose has no effect on the regeneration of hemoglobin in growing rats with iron deficiency anemia

Catani

Amâncio

Fagundes-Neto

et al. 2003

Braz J Med Biol Res

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The objective of the present study was to determine the effect of cellulose on intestinal iron absorption in rats during recovery from iron deficiency anemia. Twenty-one-day-old male Wistar-EPM rats were fed an iron-free ration for two weeks to induce anemia. At 5 weeks of age, the rats were divided into two groups (both groups receiving 35 mg of elemental iron per kg diet): cellulose group (N = 12), receiving a diet containing 100 g of cellulose/kg and control (N = 12), receiving a diet containing no cellulose. The fresh weight of the feces collected over a 3-day period between the 15th and 18th day of dietary treatment was 10.7 ± 3.5 g in the group receiving cellulose and 1.9 ± 1.2 g in the control group (P<0.001). Total food intake was higher in the cellulose group (343.4 ± 22.0 g) than in the control (322.1 ± 13.1 g, P = 0.009) during the 3 weeks of dietary treatment. No significant difference was observed in weight gain (cellulose group = 132.8 ± 19.2, control = 128.0 ± 16.3 g), hemoglobin increment (cellulose group = 8.0 ± 0.8, control = 8.0 ± 1.0 g/dl), hemoglobin level (cellulose group = 12.3 ± 1.2, control = 12.1 ± 1.3 g/dl) or in hepatic iron levels (cellulose group = 333.6 ± 112.4, control = 398.4 ± 168.0 µg/g dry tissue). We conclude that cellulose does not adversely affect the regeneration of hemoglobin, hepatic iron level or the growth of rats during recovery from iron deficiency anemia.

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“…Hepatic iron levels were determined after liquid digestion of dry tissue by atomic absorption spectrophotometry (Perkin-Elmer -5.100, Norwalk, CT, USA) (16).…”

mentioning

confidence: 99%

Dietary cellulose has no effect on the regeneration of hemoglobin in growing rats with iron deficiency anemia

Catani

Amâncio

Fagundes-Neto

et al. 2003

Braz J Med Biol Res

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“…The liver was subsequently oven dried for 22 hours at 120°C until a constant dry weight was obtained. Determination of hepatic iron was performed after acid digestion of the dry tissue using atomic absorption spectrophotometry 26 .…”

Section: Blood Sample Analysis and Experimental Proceduresmentioning

confidence: 99%

“…A total of 500mg of dried feces from each animal was weighed, divided into two samples of 250mg (duplicate) each, and subjected to acid digestion using nitric acid and perchloric acid (volumes: 4mL nitric acid and 2mL perchloric acid; digester temperature: 200°C; wavelengths: Iron=248.3nm, Calcium=422.7nm; slit: 0.7nm; flame air/acetylene; energy: Iron=60, Calcium=67). The iron and calcium analysis was performed using the Perkin-Elmer Model 5100 PC Uberlingen, Baden-Württemberg, Germany), an atomic absorption spectrophotometer 26 . To analyze iron, deionized water was added; to analyze calcium, lanthanum chloride (Merck ® , Darmstadt, Germany) was added to the samples (final concentration: 1% lanthanum).…”

Section: Apparent Absorption and Retention Of Iron And Calciummentioning

confidence: 99%

Intestinal absorption of iron and calcium from soy and cow's milk-based infant formulas in weanling rats pups

et al. 2017

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ObjectiveThis study aimed to compare the intestinal absorption of iron and calcium between soy-based and cow's milk-based infant formulas in weanling rats. MethodsTwenty male Wistar rats, twenty-one days old on the first day of weaning, were used in this experiment, divided in two Groups, one Group was fed soy protein-based infant formula the other, cow's milk protein-based infant formula. During the study period (ten consecutive days) the animals received food and water ad libitum. Hematocrit and hemoglobin were evaluated on the first, fifth, and tenth days by the Wintrobe and cyanomethemoglobin methods. Feces and urine were collected, beginning on the fifth day, for three consecutive days. On the tenth day, hepatic iron content was also analyzed. Hepatic iron as well as fecal and urinary iron and calcium analyses were performed using an atomic absorption spectrophotometer. At thirty-one days of age, the animals were anesthetized with ketamine and xylazine and sacrificed by exsanguination via the vena cava.

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“…Foi realizada digestão líquida prévia com HNO 3 (ácido nítrico) e HCLO 4 (ácido perclórico) e os minerais determinados por espectrofotometria de Absorção Atômica em aparelho Perkin Elmer modelo 5.100 nas seguintes condições: Comprimento de onda: Cu = 324,8nm; Mg = 285,2nm; Zn = 213,9nm; Fe = 248,3nm; Ca = 422,7nm; fenda 0,7 nm; chama ar/acetileno; energia: Cu = 71; Mg = 69; Zn = 65; Fe = 60; Ca = 67; leitura em duplicata de 10 amostras de cada tipo de dieta com tempo de integração de 2 segundos (MARKS et al, 1971). Os resultados foram expressos em μg metal/ 100g dieta e desvio padrão.…”

Section: Mineraisunclassified