Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of <i>Arisaema jacquemontii</i> Blume

Baba, Shoib Ahmad; Parah, Shabir A.

doi:10.1016/j.jtusci.2014.11.001

Cited by 546 publications

(374 citation statements)

References 25 publications

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“…In order to confirm the synthesis of total flavonoids by the endophytic fungi present in the L. sidoides and plant extract (Table 2), extracts were obtained with the solvent ethyl acetate (AcOEt) to extract compounds with recognized antimicrobial activity, such as phenols and flavonoids (Baba and Malik, 2015).…”

Section: Discussionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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Lippia sidoidesCham. (Verbenaceae) is a species native to the Brazilian northeast, widely used in popular medicine. Its leaves were used for the isolation of endophytic fungi and extraction of metabolites. Among them, three were selected according to fungitoxicity tests against the maize phytopathogenic fungus, Curvularia lunata (Wakker). However, the objective of this study was to identify the role of L. sidoides extracts associated with their endophytic fungi, necessary to reduce excess of fungicides applied on the maize crop. Metabolites were evaluated for antioxidant activity by 2.2-diphenyl-1-picrylhydrazyl (DPPH), phenols, total flavonoids and one of it endophytic fungus were evaluated for synergism (Verticilium sp. and plant extracts). The endophytic fungi and plant extracts evaluated for phenolic content ranged from 0.29 ± 0.05 to 96.94 ± 11.86 mgEAG/g, the content of flavonoids from 14.31 ± 1.56 to 192.33 ± 4.58 mgER/g, and antioxidant activity could only be observed for the plant extract with EC 50 81 ± 0.3%. The secondary metabolites identified by HPLC in the plant extract were catechin, quercetin, gallic acid and naringin. Naringenin, catechin, epigallocatechin gallate and quercetin were identified in the extract of the fungi viz. Verticillium sp. and Fusarium sp. Synergistic analysis between a 1:1 proportion of plant and fungal extracts has shown more efficient (79.0%) inhibition of C. lunata. Thus, alternative control of phytopathogenic fungi can be accomplished using plant extracts associated with their endophytic fungi, reducing the excess of fungicides applied on the maize crop.

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Section: Discussionmentioning

confidence: 99%

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Ferreira¹,

Santos²,

Soares³

et al. 2017

J. Med. Plants Res.

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“…21 As their free radical scavenging ability is facilitated by their hydroxyl groups, the total phenolic concentration could be used as a basis for rapid screening of antioxidant activity. 22 In addition, our previous study found that Hawm Nil rice (dark purple rice) had the highest TPC when compared with other color rice and cow's milk. 6 Other research of pigment rice also reported that dark purple or black rice had high TPC compared to other color rice without fermentation.…”

Section: Lactic Acid and Alcohol Concentrationmentioning

confidence: 95%

“…Plants rich in secondary metabolites including phenolics, flavonoids and carotenoids have antioxidant activity due to their redox properties and chemical structures (Baba and Malik, 2015). Results showed that the Hawm Nil rice milk kefir had higher antioxidant activity (33.32±6.48 and 1.33±0.60;%; respectively) than cow's milk kefir at 72 h Table 3.…”

Section: Antioxidant Activitiesmentioning

confidence: 98%

Characteristics of Thai Pigmented Rice Milk Kefirs with Potential as Antioxidant and Anti-Inflammatory Foods

Deeseenthum¹,

Luang-In²,

Chunchom³

2017

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“…A set of reference standard solutions of rutin (25,50,75, 100 and 250 μg/ml) were prepared in the same manner as described earlier. The absorbance of test and standard solutions were determined against the blank at 510 nm using UV/Visible spectrophotometer [20] . Based on the measured absorbance, the concentration of flavonoids in the extract was read (mg/ml) from equation obtained from the Rutin calibration line and was expressed was expressed as mg of RE/g of extract.…”

Section: Total Flavonoid Content Estimation (Tfc)mentioning

confidence: 99%

Comparative Evaluation of Crude Extract Fractions of the Whole Plant of Taxillus heyneanus and Dalechampia indica for Antioxidant Activity, Total Phenolic and Flavonoid Content

S¹,

Eswaraiah²

2017

IOSRPHR

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This study was aimed to investigate the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant potential of ethanolic fraction (ETH&EDI) and chloroform fraction (CTH & CDI) of crude extracts of the whole plant of Taxillus heyneanus (TH) and Dalechampia indica (DI). values (38.75 ±0.83, 62.67±5.23, 80.89±0.47μg ⁄ ml values(477.61±0.69, 404.00±0.38 & 505.52±4.83 μg ⁄ml) values for the DPPH, Hydrogen peroxide and nitric oxide. Ethanolic fractions of both plants (ETH & EDI) exhibited significant scavenging activities with good amounts of phenolic and flavonoid compared to its chloroform fractions (CTH & CDI).The results of the study show that ethanolic fraction of Taxillus heyneanus possesses better antioxidant activity compared to the ethanolic fraction of Dalechampia indica which could be attributed to the presence of high amount of phenolic and flavonoids. Keywords: Antioxidant activities, Dalechampia indica, Taxillus heyneanus, whole plant I. INTRODUCTOIONThe human body is a wonderful machine which requires fuel named oxygen for its function. It plays a pivotal role in the energy metabolism of the body which involves various physical and chemical processes [1] .While oxygen supports our life, it also produces free radicals (ROS) in the body as the waste products. Oxygencentered free radicals (ROS) includes superoxide anion (O 2 .-), hydroxyl radical (.OH), hydrogen peroxide (H 2 O 2 ), peroxyl, hypochlorous acid and nitric oxide [2] . These are highly reactive unstable molecules which have a hunger for electrons, reacts with biomolecules (lipids, proteins, DNA etc.) and alters its structure and function. This, in high levels, leads to oxidative stress [3,4] .Oxidative stress occurs when our body defense systems fail to wipe off this excess amount of free radicals. These free radicals spring from different sources such as endogenous (mitochondria, peroxisomes, endoplasmic reticulum etc.) and exogenous (pollution, ionization radiation etc.) sources [5] .It has implications for a broad range of disorders including stroke, ischemia-reperfusion injury, arthritis, cancer, diabetes, neurodegenerative diseases, spinal cord injury, cardiovascular diseases, sepsis, inflammation and respiratory diseases [6,7] .Here comes the concept of antioxidants, nature's way of cell defense against free radical (ROS) attack. These antioxidants synthesized invivo (SOD, catalase, glutathione peroxidase) or taken as dietary antioxidants (vitamin C, vitamin E, β-carotene and selenium) either quenches or scavenges free radicals (ROS) and repairs the damages caused by them [8] .The quest for effective and beneficial natural antioxidants has been increased nowadays as the synthetic antioxidants like butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) found to be harmful [9] .Plants are found to be a good source of natural antioxidants as they can synthesize phytoconstituents such as phenolics, tannins, flavonoids etc. in different parts which have the ability to scavenge free radicals [10].T...

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Determination of total phenolic and flavonoid content, antimicrobial and antioxidant activity of a root extract of Arisaema jacquemontii Blume

Cited by 546 publications

References 25 publications

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Secondary metabolites from endophytic fungus from Lippia sidoides Cham.

Characteristics of Thai Pigmented Rice Milk Kefirs with Potential as Antioxidant and Anti-Inflammatory Foods

Comparative Evaluation of Crude Extract Fractions of the Whole Plant of Taxillus heyneanus and Dalechampia indica for Antioxidant Activity, Total Phenolic and Flavonoid Content

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