Determination of Total Flavonoid in Jamu “X” With Uv-Visible Spectrophotometric Methods

Haresmita, Perdana Priya; Pradani, Missya Putri Kurnia

doi:10.31603/pharmacy.v8i2.6864

Cited by 3 publications

(2 citation statements)

References 10 publications

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“…Ditimbang 2,05 gram natrium asetat 82,03 g/mol lalu dilarutan dalam 25 ml akuades [15]. Ditimbang 1 gram AlCl3 kemudian dilarutkan dengan 10 ml akuades di dalam lemari asam [16].…”

Section: Pengujian Flavonoid Totalunclassified

Perbandingan Flavonoid Total Ekstrak Sirih Cina (Peperomia pellucida L. Kunth) dengan Variasi Konsentrasi Etanol Menggunakan Spektrofotometri UV-Vis

Puspa Pratiwi,

Cahya Anggreni,

Dharma Yanti

et al. 2023

JSSCR

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Chinese betel can be used to lower cholesterol, heal broken bones, rheumatoid arthritis, anti-fungal medication, gout, protect the digestive system, treat urinary tract disorders and so on. Chinese betel plants contain active compounds such as terpenoids, flavonoids, alkaloids, saponins and phenols. Flavonoids are natural chemicals found in plants. Flavonoids also have antioxidant properties to reduce the effects of free radicals. The research aims to determine the total flavonoid content of Chinese betel extract with variations of 70%, 80% and 96% ethanol using the UV-Vis spectrophotometric method. This method was carried out by quantitative analysis of the total flavonoid content of Chinese betel ethanol extract using UV-Vis spectrophotometry and qualitative analysis using concentrated Mg and HCL powder. The material used is Chinese betel extract (Peperomia pellucida) obtained from Jembrana district, Bali province. Other materials used are 70% ethanol, 80% ethanol, 96% ethanol and ethanol p.a., sodium acetate, distilled water, AlCl3, quercetin, Mg powder and concentrated HCL. The results of testing the total flavonoid content in Chinese betel ethanol extract varied in concentration of 70%, namely containing flavonoids of 2.78576 g/100g EQ, 80% ethanol Chinese betel extract containing flavonoids of 5.132503 g/100g EQ, and Chinese betel ethanol extract 96 % contains flavonoids of 6.77682 g/100g EQ. The highest flavonoid content results were obtained by the 96% ethanol concentration extract because 96% ethanol has polar properties so it can attract flavonoid compounds well. Qualitative analysis of flavonoids showed positive results with the formation of a brownish red color.

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“…Ditimbang 2,05 gram natrium asetat 82,03 g/mol lalu dilarutan dalam 25 ml akuades [15]. Ditimbang 1 gram AlCl3 kemudian dilarutkan dengan 10 ml akuades di dalam lemari asam [16].…”

Section: Pengujian Flavonoid Totalunclassified

Perbandingan Flavonoid Total Ekstrak Sirih Cina (Peperomia pellucida L. Kunth) dengan Variasi Konsentrasi Etanol Menggunakan Spektrofotometri UV-Vis

Puspa Pratiwi,

Cahya Anggreni,

Dharma Yanti

et al. 2023

JSSCR

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“…The sample was then extracted with a magnetic stirrer at 50°C for 60 minutes after receiving up to ethanol 80% 25 ml. In order to attain a level of 1000 ppm [14], the sample was filtered using filter paper and then deposited in volume to the limit mark [15].…”

Section: Preparation Of Samplesmentioning

confidence: 99%

Flavonoid Level Determination in Jamu Pegel Linu in Magelang Regency Using Uv-Visible Spectrophotometry

Pratiwi,

Haresmita,

Pradani

et al. 2023

J. Trop. Pharm. Chem.

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Jamu is made from the toga plant, which is still used medicinally in Indonesia. Rutin, a type of flavonoid component, is frequently present in herbal medicine pegel linu. Rutin serves as an antioxidant and anti-inflammatory by neutralizing free radicals, which helps to prevent tissue damage and relieve soreness. This research's objective was to evaluate the amounts of flavonoids in samples using UV-Visible spectrophotometry, employing three samples of herbal medicine (A, B and C) as well as the standard solution, reagents AlCl3 and sodium acetate. In this investigation, the protocol utilized to determine the maximum wavelength was 400–800 nm, and the result was 413.5 nm. The linear regression equation is y = 0.0471x + 0.0624 with a correlation coefficient of R2 of 0.9934 and a LOD value of 3.9694 mg / L and a LOQ value of 13.231 mg / L. The determination of total flavonoid levels considered as rutin uses concentrations of 10, 12.14, 16 and 18 ppm with operating time of 30 minutes. The findings of rutin content analysis for samples A, B, and C were 1.6683%, 2.8763%, and 3.0923%, respectively.

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Antioxidant activity, total phenolics, flavonoids, tannin content of cassava leaf (Manihot esculenta Crantz) with different cooking and drying methods

Diniyah,

Kostrada,

Marchianti

2024

AIP Conference Proceedings

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Determination of Total Flavonoid in Jamu “X” With Uv-Visible Spectrophotometric Methods

Cited by 3 publications

References 10 publications

Perbandingan Flavonoid Total Ekstrak Sirih Cina (Peperomia pellucida L. Kunth) dengan Variasi Konsentrasi Etanol Menggunakan Spektrofotometri UV-Vis

Perbandingan Flavonoid Total Ekstrak Sirih Cina (Peperomia pellucida L. Kunth) dengan Variasi Konsentrasi Etanol Menggunakan Spektrofotometri UV-Vis

Flavonoid Level Determination in Jamu Pegel Linu in Magelang Regency Using Uv-Visible Spectrophotometry

Antioxidant activity, total phenolics, flavonoids, tannin content of cassava leaf (Manihot esculenta Crantz) with different cooking and drying methods

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