Time, fertilizer, tillage, and cropping systems may alter soil organic carbon (SOC) levels. Our objective was to determine the effect of long-term cropping systems and fertility treatments on SOC. Five rotations and two N fertility levels at three Iowa sites (Kanawha, Nashua, and Sutherland) maintained for 12 to 36 yr were evaluated. A 75-yr continuous corn (Zea mays L.) site (Ames) with a 40-yr N-P-K rate study also was evaluated. Soils were Typic and Aquic Hapludolls and Typic Haplaquolls. Four-year rotations consisting of corn, oat (Avena saliva L.), and meadow (alfalfa [Medicago sativa L.], or alfalfa and red clover [Trifolium pratense L.]) had the highest SOC (Kanawha, 32.1 g/kg; Nashua, 21.9 g/kg; Sutherland, 27.9 g/kg). Corn silage treatments (Nashua, < 18.9 g/kg; Sutherland, <23.2 g/kg) and no-fertilizer treatments (Kanawha, 25.3 g/kg; Nashua, <20.9 g/kg; Sutherland, <23.5 g/kg) had the lowest SOC. A corn-oat-meadowmeadow rotation maintained initial SOC (27.9 g/kg) after 34 yr at Sutherland. Continuous corn resulted in loss of 30% of SOC during 35 yr of manure and lime treatments. SOC increased 22% when N-P-K treatments were imposed. Fertilizer N, initial SOC levels, and previous management affected current SOC levels. Residue additions were linearly related to SOC (Ames, r 2 = 0.40; Nashua, r 2 = 0.82; Sutherland, r 2 = 0.89). All systems had 22 to 49% less SOC than adjacent fence rows. Changing cropping systems to those that conserve SOC could sequester as much as 30% of C released since cropping began, thereby increasing SOC. T HE ATMOSPHERIC CC>2 CONCENTRATION has gained much attention for its potential contribution to global warming. Agriculture affects atmospheric COz concentrations through consumption of fossil fuels, clearing of forested lands for food production (U.S. Congress, 1991; Wallace et al., 1990), and alteration of SOC levels by agricultural management practices. Agricultural fuel consumption and N fertilizer production release 35.4 Tg C yr~' into the atmosphere. Based on current production practices, the Council for Agricultural Science and Technology (1992) estimates another 2.7 Tg C yr~' are released into the atmosphere from cultivated soils in the USA alone. Changes in SOC can be attributed to crop species grown, cropping systems (including rotations), residue management practices, fertilizer applications, tillage practices, and other management factors (Havlin et al., 1990; Unger, 1968). Anderson et al. (1990), Bauer and Black (1981), and Havlin et al. (1990) independently showed that SOC losses were directly related to tillage intensity. Manure applications modified the tillage-SOC relation, increasing SOC even with high-intensity conventional tillage (Anderson et al., 1990). Crop rotations may retard SOC losses relative to those observed in