Determination of tocopherols in vegetable oil samples by non-aqueous capillary electrophoresis (NACE) with fluorimetric detection

Galeano-Dı́az, Teresa; Acedo-Valenzuela, María Isabel; Silva-Rodríguez, Antonio

doi:10.1016/j.jfca.2011.04.014

Cited by 32 publications

(25 citation statements)

References 35 publications

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“…vitamins B) or insoluble (e.g. tocopherols) vitamins in different food matrices . Besides, the determination of vitamin C by CZE in tomato samples has demonstrated to be a very useful way to teach students of undergraduate labs the usefulness of CE as analytical technique .…”

Section: Vitaminsmentioning

confidence: 99%

“…These compounds are relevant to food science and nutrition due to their nutritional functions and ability to prevent the oxidation of vegetable oils. A novel NACE method for the determination of tocopherols in vegetable oil samples (maize germ, olive, and sunflower) has been recently reported . On‐line UV and fluorescence detection were employed, demonstrating the latter an improved sensitivity and selectivity.…”

Section: Vitaminsmentioning

confidence: 99%

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Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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In this work, the analysis of foods and food components using capillary electromigration methods is reviewed. The present work presents and discusses the main CE applications performed in Food Science and Technology including the new field of Foodomics, reviewing recent results on food quality and safety, nutritional value, storage, bioactivity, as well as applications of CE for monitoring food interactions and food processing. The CE analysis of a large variety of food-related molecules with different chemical properties, including amino acids, peptides, proteins, phenolic compounds, carbohydrates, DNA fragments, vitamins, toxins, pesticides, additives, and other minor compounds is described. The use of microchips, CE-MS, and chiral-CE in food analysis is also discussed as well as other current and foreseen trends in this area of research. Following the previous review by Castro-Puyana et al. (Electrophoresis, 2012, 33, 147–167), the current review covers the papers that were published from February 2011 to February 2013.

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Section: Vitaminsmentioning

confidence: 99%

Section: Vitaminsmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

et al. 2013

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“…The second one is based on their spectroscopic properties. The spectroscopic methods have been used to distinguish diverse edible oils [2], to characterize oil designations, and to study the spectroscopic and thermochemical properties of the vitamin E [14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

TDDFT prediction of UV–vis absorption and emission spectra of tocopherols in different media

et al. 2015

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We use the TDDFT/PBE0/6-31+G* method to determine the electronic absorption and emission energies, in different media, of the four forms of tocopherol, which differ by the number and the position of methyl groups on the chromanol. Geometries of the ground state S0 and the first singlet excited state S1 were optimized in the gas phase, and various solvents. The solvent effect is evaluated using an implicit solvation model (IEF-PCM). Our results are compared to the experimental ones obtained for the vitamin E content in several vegetable oils. For all forms of tocopherols, the HOMO-LUMO first vertical excitation is a π-π* transition. Gas phase and non-polar solvents (benzene and toluene) give higher absorption wavelengths than polar solvents (acetone, ethanol, methanol, DMSO, and water); this can be interpreted by a coplanarity between the O-H group and the chroman, allowing a better electronic resonance of the oxygen lone pairs and the aromatic ring, and therefore giving an important absorption wavelength, whereas the polar solvents give high emission wavelengths comparatively to gas phase and non-polar solvents. Fluorescence spectra permit the determination, the separation, and the identification of the four forms of tocopherols by a large difference in emission wavelength values. Graphical Abstract Scheme from process methodological to obtain the absorption and emission spectra for tocopherols.

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“…However, this step can be bypassed when NACE is used. Some of the most important matrices for which NACE has been employed are pharmaceutical drugs, food material, and environmental samples [27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

“…Thus, more sensitive detectors are being developed, like MS [24,26], laserinduced fluorescence [28], electrochemical detection [36] and fluorescence (FL) [32]. Recently, Galeano-Díaz et al [32] have carried out the determination of tocopherols in vegetable oils using BGE containing cholate, borate buffer, and sodium hydroxide in methanol, with good results of sensitivity and selectivity using FL detection. MS detectors are being increasingly used after many forms of separation due to their near-universality, their ability to elucidate or confirm structures, and their high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

et al. 2012

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Response surface methodology has been applied to the optimization of a simple and rapid non-aqueous capillary electrophoresis method for the separation and determination of several phenolic compounds belonging to the different families present in olive oil. A Box-Behnken design was employed and a total of 27 experiments were performed using olive oil samples spiked with the phenols and injected directly in the capillary after dilution 1:1 with 1-propanol. Finally, the background electrolyte (BGE) was constituted of 25 mM boric acid and 18 mM KOH in a mixture of 74:26 (v/v) 1-propanol/methanol. The hydrophobicity of the BGE allows its miscibility with the olive oil and, as a consequence, the possibility of characterizing and determining these kinds of compounds in this sample without any pretreatment. A hydrodynamic injection (6 s, -30 mbar) was applied and the separation was carried out using 35 °C and +20 kV of separation temperature and voltage, respectively. A capillary with two detection windows for serial online UV and fluorescence detection was satisfactorily employed. The validation of the method was carried out by setting the calibration curves, and the figures of merit were finally obtained. A lineal relationship between the corrected peak area and concentration and limits of detection in the order of micrograms per milliliter were found.

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Determination of tocopherols in vegetable oil samples by non-aqueous capillary electrophoresis (NACE) with fluorimetric detection

Cited by 32 publications

References 35 publications

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

TDDFT prediction of UV–vis absorption and emission spectra of tocopherols in different media

Development of a non-aqueous capillary electrophoresis method with UV–visible and fluorescence detection for phenolics compounds in olive oil

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