Determination of the thickness of electron microscopy sections

Zelander, T.; Ekholm, R.

doi:10.1016/s0022-5320(60)80031-7

Cited by 20 publications

(15 citation statements)

References 8 publications

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“…Bachmann and Sitte (1960) used the Tolansky multiple-beam interferometric method, and arrived at the following results : grey, 300-500 A; silver, 500-800 A; gold, 800-1000 A. Zelander and Ekholm (1960) determined the thickness of methacrylate sections (cut on two ultramicrotomes with a thermal advance) by the interference-microscopical method. They found that the thickness of sections of "the characteristic greyish tone" varied from 340 A to 740 8.…”

mentioning

confidence: 99%

Studies on thickness variation in ultrathin sections for electron microscopy

Williams

Meek

1966

Journal of the Royal Microscopical Society

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confidence: 99%

Studies on thickness variation in ultrathin sections for electron microscopy

Williams

Meek

1966

Journal of the Royal Microscopical Society

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“…a plastic deformation resulting in sections which are thicker than the actual feeding of the microtome (cf. Zelander & Ekholm, 1960; (1) y, and can be calculated (2) (cf. Colding, 1959), and if Vestopal is assumed to be isotropic, the following relationship probably represents a good approximation (Colding, 1959) : 4 + py = 45".…”

Section: Discussionmentioning

confidence: 99%

Some observations on knife properties and sectioning mechanics during ultramicrotomy of plastic embedding media

Helander

1974

Journal of Microscopy

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The edge and the trough surface of ultramicrotome glass knives were studied by interference light microscopy and electron microscopy. Particular attention was paid to the region which is commonly used for microtomy; here the bevel angle was calculated to 50-55". The edge was serrated, even in the region used for sectioning, where the indentations measured up to 20 nm in depth. The imprints of edge serrations left on the sectioned material (Vestopal) were studied by electron microscopy. Different embedding materials displayed different degrees of compression when sectioned in the ultramicrotome: with Epon the compression was less than loo!,, whereas methacrylate was compressed by more than 30o/b. Different trough fluids were tested: aqueous solutions of ethanol, acetone and dioxane gave less compression than pure water. However, the compression could also be reduced by exposing compressed Vestopal sections to these solutions. The direction and magnitude of the cutting force was measured with transducers during the sectioning of Vestopal blocks. Thus -0.3 pm wide sections cut at a feeding rate of 80 nm and a speed of 2 mm/sec required a cutting force of about 8 mN. The direction of the forces was 8-15' from the plane of cutting.

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“…The mean fold thickness was about 20% thinner than that determined by the caliper method, an expected result of sublimation of plastic or heat-induced thinning in the electron beam (Gunning and Hardham, 1977;Peachey, 1958;Silverman et al, 1969;Zelander and Ekholm, 1960). We used the caliper determined thickness C'z''-axis) for all reconstructions and volume calculations.…”

Section: Serial Sectioningmentioning

confidence: 99%

Three‐dimensional reconstruction and quantitative analysis of rat lung type II cells: A computer‐based study

Young¹,

Fram

Craig³

1985

Am. J. Anat.

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The three-dimensional structure of alveolar epithelial type II cells was imaged using a computer-based system designed for reconstruction and quantitative analysis of serially sectioned specimens. Six type II cells were reconstructed from serial ultrathin sections of lungs from two Sprague Dawley male rats and the results were compared to standard morphometric estimates of type II cell composition from five other Sprague Dawley male rats. A minor portion of the type II cell surface was in contact with the alveolar airspace while most of the cell surface was embedded in the alveolar septal interstitium. The type II cells contained multiple Golgi regions located close to the nucleus. Mitochondria formed a few branching filamentous networks extending throughout the cell. The reconstructed cells appeared to represent a homogeneous population having fractional volumes of intracellular organelles very similar to those found by morphometric techniques. The spatial distribution of secretory organelle volume suggests that the organization of this cell type reflects an ordered progression of secretory particle maturation which is consistent with earlier hypotheses of lamellar body assembly.

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Determination of the thickness of electron microscopy sections

Cited by 20 publications

References 8 publications

Studies on thickness variation in ultrathin sections for electron microscopy

Studies on thickness variation in ultrathin sections for electron microscopy

Some observations on knife properties and sectioning mechanics during ultramicrotomy of plastic embedding media

Three‐dimensional reconstruction and quantitative analysis of rat lung type II cells: A computer‐based study

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