Determination of the neurolathyrogen β-N-oxalyl-l-α,β-diaminopropionic acid using high-performance liquid chromatography with fluorometric de

Geda, A. K.; Briggs, Colin J.; Venkataram, S.

doi:10.1016/0021-9673(93)80378-l

Cited by 26 publications

(15 citation statements)

References 9 publications

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“…Most of the previous analytical methods were developed for the determination of dencichine in food or seed samples [8][9][10][11][12][13]. And the recent reports about the determination of dencichine were all using the method of derivatization [4,14,15].…”

Section: Introductionmentioning

confidence: 99%

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

Chen¹,

Yuan²,

He³

et al. 2012

AJAC

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In order to quantitate dencichine in biological samples, a selective and sensitive method for the determination of dencichine in rat plasma based on high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was developed and validated. (l)-2-amino-3-(carboxymethylthio)propionic acid was used as the internal standard (I.S.). After a protein precipitation extraction with acetonitrile, dencichine and the I.S. were chromatographed on an Xterra MS-C18 column. The mobile phase was consisted of 20mmol/L ammonium acetate solution-acetonitrile (35:65, V/V) at a flow rate of 0.2 mL/min. The detection was performed on a triple quadrupole mass via electrospray ionization (ESI) source in the positive mode. The intra-and inter-day precision (relative standard deviation, R.S.D.) values of dencichine were below 6.7%. The extraction recoveries were up 85%. The lower limit of quantification was 20 ng/ml, which was sensitive enough to detect the analyte. The HPLC-MS/MS method was successfully applied to the pharmacokinetic study after an intravenous administration of dencichine in Sprague-Dawley (SD) rat.

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Section: Introductionmentioning

confidence: 99%

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

Chen¹,

Yuan²,

He³

et al. 2012

AJAC

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“…Consumption of lathyrus by humans has been linked to the incidence of neurolathyrism, a "paralytic disease of the lower limbs, which sets in gradually and progresses irreversibly due to the cumulative actions of the various toxins" Murti (1986). The specific factor responsible is currently identified as B-N-oxalyl-L-a, B-diaminopropionic acid (ODAP), its synonym B-N-oxalylamino-L-alanine (BOAA) or the preferred IUPAC nomenclature L-2-amino-3-oxalylaminopropanoic acid (Geda et al 1993).…”

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confidence: 99%

Evaluation of lathyrus (Lathyrus sativus L) as an ingredient in pig starter and grower diets

Castell¹,

Cliplef²,

Briggs³

et al. 1994

Can. J. Anim. Sci.

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. 1994. Evaluation of lathyrus (Inth|rus sativus L.) as an ingredient in pig startiiand grower diets. Cun. J. Anim. . Seed from Inthyys sctivus L., with a low contenlof 6-N-oxalyj-L-a,p-diamino-propionic acid (ODAP), was evaluated as an,ingredient (LM) in swine-die^ts. The first experiment involved +5 pigs feO LM, coniainin g27 % protein (CP) and 3 g OD{P !g -.3t 0. 10, 20, 30 and 4O% of sqart-e1 liets (i0% CP) over a 6-wk'pEriod. In exp. 2, M;types bf f-U (O.q and,2.1 g ODAP kg ').were each included as 0, 10' 20 and 30 of mash diets fed to 84 pigs from jS to tOO kg liveweight.. The effects of pelleting and_supplementation with I and 2 g methionine kg-r, for LM diets Jontaining < 0.5 gbOel tg '. were examined in the final experiment using 80 groYlq finishing pigsllncreasing the LM content aSove 10% of the startEr diet reduced average daily.gains (A-DG) and feed intake (A?FI) but feed"6oiversion (gain/feed; G:F) was unaffected (P > 0.10). In exp. 2, ADG declined as the dietary content of either LM increased. The lower-bDAP LM-fed pigs had relatively higher ADFI (P < 0.01) and ADG (P < 0.05) but slightty lower G:F (P < 0.08). Consumption of lathyrujled to higher (P < 0.01) liver and kidney weightg 11d ]ow91 (P < 0.05) protein content in the longissimus doisi. Pelleting the diet impioved ADG (P < 0.01) and G:F (P < 0.05) but did not prevent the LM-effect on growtlirates. Increasing the tdtal sulfur amino acid content of the diet from 0.48 toO.66% had no beneficial effect on live or carcass measurements. [Jsing a method capable of detecting 100 ng ODAP per g fresh tissue, no_ residues were found in the loin muscle of LM-fed pigs. Th"e results sugg'est that anti-nutritive constituents (e.g. anti-trypsin and anti-chymotrypsin), rather than ODAP. limit the potential of lathvrus seed as a feedstuff for swine.Key words: Pig, lathyrus, growth, carcass, neurotoxin Castell, A. G., Cliplef, R. L., Briggs, C. J., Campbell, C. G. et Bruni, J. E. 1994 de TG au-deli de lO% de I'aliment de d6marrage provoquait un abaissement du GMQ et de I'ing6r6 alimentaire, mais I'indice de conversion n'6tait pas touch6 (P > 0,10). Dani I'exp6rience deux, le GMQ diminuait h mesure.que la proportion.des deux tourteaux de gesse augmentait. Les porcs consommant le tourteau de gesse d basse teneur en ODAP manifestaient un taux d'ingestion et un GMQ ielativerient plus 6lev6s (respectivement P < 0,01 et P < 0,05), mais un indice de conversion l6gdrement moins bon (P < O,OS). I-u consommation de geise entrainait I'augmentation (P < 0,01) du poids du_foie et des reins, mais une baisse (P i 0,05)'deia teneur en prot6ines d"u longissimus dorsilLa pr6sentation de ta ration sous forme d'agglom6r!am6liorait le GMQ (P < 0,01) et I'indice de consommation (P < 0,05), mais ne r6ussissait pas d contrer I'effet n6gatif du TG sur le.taux de croissance. L'accroissement de la teneur en acides amin6s soufr6s totaux de la ration de 0,48 d.0,66% n'avait pas d'effet favorable sur les performances de I'animal ni sur les mesures de la carcasse. L'utilisation d'une m6th...

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“…In addition to the experimental inconvenience and time-consuming derivatization steps, lack of selectivity [14,15], difficulty of separating the derivatizing reagent [16], and easy decomposition of the derivatives [17] were observed. Capillary zone electrophoresis was also considered with the drawback that the higher working pH (9.2) causes hydrolysis of ODAP to DAP [18].…”

Section: Introductionmentioning

confidence: 99%

“…High performance liquid chromatographic (HPLC) methods involving off-line pre-column derivatization with dansyl chloride [14], 9-fluorenylmethyl chloroformate [15], phenyl isothiocyanate (PITC) [16], and 6-aminoquinolyl-Nhydroxy-succinimyl carbamate (AQC) [17] were also reported. In addition to the experimental inconvenience and time-consuming derivatization steps, lack of selectivity [14,15], difficulty of separating the derivatizing reagent [16], and easy decomposition of the derivatives [17] were observed.…”

Section: Introductionmentioning

confidence: 99%

Development of an Amperometric Biosensor for β‐N‐Oxalyl‐L‐α,β‐Diaminopropionic Acid (β‐ODAP)

2004

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An amperometric method for the determination of the neurotoxic amino acid b-N-oxalyl-l-a,b-diaminopropionic acid (b-ODAP) using a screen printed carbon electrode (SPCE) is reported. The electrode material was bulkmodified with manganese dioxide and used as a detector in flow injection analysis (FIA). The enzyme glutamate oxidase (GlOx) was immobilized in a Nafion-film on the electrode surface. The performance of the biosensor was optimized using glutamate as an analyte. Optimum parameters were found as: operational potential 440 mV (vs. Ag/ AgCl), flow rate 0.2 mL min , respectively, with corresponding relative standard deviations of 3.3 and 4.5%. The biosensor retained more than 73% of its activity after 40 days of on-line use.

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Determination of the neurolathyrogen β-N-oxalyl-l-α,β-diaminopropionic acid using high-performance liquid chromatography with fluorometric de

Cited by 26 publications

References 9 publications

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

A Sensitive HPLC-MS/MS Analysis of Dencichine in Rat Plasma and Its Application to Pharmacokinetics

Evaluation of lathyrus (Lathyrus sativus L) as an ingredient in pig starter and grower diets

Development of an Amperometric Biosensor for β‐N‐Oxalyl‐L‐α,β‐Diaminopropionic Acid (β‐ODAP)

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