Development of an Amperometric Biosensor for β‐N‐Oxalyl‐L‐α,β‐Diaminopropionic Acid (β‐ODAP)

Abstract

An amperometric method for the determination of the neurotoxic amino acid b-N-oxalyl-l-a,b-diaminopropionic acid (b-ODAP) using a screen printed carbon electrode (SPCE) is reported. The electrode material was bulkmodified with manganese dioxide and used as a detector in flow injection analysis (FIA). The enzyme glutamate oxidase (GlOx) was immobilized in a Nafion-film on the electrode surface. The performance of the biosensor was optimized using glutamate as an analyte. Optimum parameters were found as: operational potential 440 mV (vs. Ag/ AgCl), flow rate 0.2 mL min , respectively, with corresponding relative standard deviations of 3.3 and 4.5%. The biosensor retained more than 73% of its activity after 40 days of on-line use.

l-Glutamate biosensor for estimation of the taste of tomato specimens

“…MnO 2 has also been used as the mediator for indirect glutamate determination, i.e. by detecting hydrogen peroxide [26]. Direct determination of the substrate, however, results in better stability of the enzyme and a more precise response of the sensor.…”

“…For this purpose different mediators-electron-transfer complexes, e.g. Os complexes [12,13,[23][24][25] or tetrafulvalene-tetracyanoquinodimethane complex (TTF-TCNQ) [26,27]-were introduced for direct substrate oxidation:…”

Determination of β-ODAP by various methods with special emphasis on biosensors: A mini-review

“…Detection limits (as 3 value) for both analytes were 9.12 and 111.0 M with corresponding relative standard deviations of 3.3 and 4.5% respectively. The biosensor retained more than 73% of its activity after 40 days of on-line use [55].…”

“…Few amperometric ODAP biosensors have been reported in the literature [50][51][52][53][54][55][56], which act on the following principle: 2.6.1.1. Principle.…”

Construction and application of β-(3-N-oxalyl-l-2,3-diaminopropanoic acid) biosensor based on carboxylated multiwalled carbon nanotubes/gold nanoparticles/chitosan/Au electrode

“…However, all of these methods suffered from glutamate interference. Either liquid chromatography (LC) 19,20 or an enzymatic method using glutamate decarboxylase 21 were employed to remove glutmate interference but it was time-consuming, expensive and complicated due to the enzyme/protein. Moreover, most of these methods did not satisfy the requirements for a simple, fast, accurate and specic method.…”