Determination of the MRI contrast agent Gd-DTPA by SEC?ICP?MS

Loreti, Valeria; Bettmer, Jörg

doi:10.1007/s00216-004-2700-4

Cited by 39 publications

(28 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While RAPTA-T preferred binding to hTf over HSA, cisplatin was found to interact with both proteins to a similar extent. Similar investigations were also performed on gadolinium [ 29 , 30 ], titanium [ 31 ], and tungsten compounds [ 32 ], among others [ 3 ].…”

Section: Introductionmentioning

confidence: 79%

Serum-binding properties of isosteric ruthenium and osmium anticancer agents elucidated by SEC–ICP–MS

et al. 2018

View full text Add to dashboard Cite

Size-exclusion chromatography–inductively coupled plasma–mass spectrometry (SEC–ICP–MS) was used to study the serum-binding preferences of two metallodrugs with anticancer activities in vivo, namely the organoruthenium compound plecstatin-1 and its isosteric osmium analog. The complexes were administered intraperitoneally into mice bearing a CT-26 tumor. Comparing the total metal content of mouse whole blood and serum underlined that the metallodrugs are mainly located in serum and not in the cellular fraction of the blood samples. In mouse serum, both compounds were not only found to bind extensively to the serum albumin/transferrin fraction but also to immunoglobulins. Free drug was not observed in any of the samples indicating rapid protein binding of the metallodrugs. These findings were validated by spiking human serum with the respective compounds ex vivo. An NCI-60 screen is reported for the osmium analog, which revealed a relative selectivity for cancer cell lines of the ovary and the central nervous system with respect to plecstatin-1. Finally, a COMPARE 170 analysis revealed disruption of DNA synthesis as a possible treatment effect of the osmium-based drug candidate.Graphical abstract

show abstract

Section: Introductionmentioning

confidence: 79%

Serum-binding properties of isosteric ruthenium and osmium anticancer agents elucidated by SEC–ICP–MS

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Although reversed phase chromatography (RP-HPLC) accounts for only very little retention for polar compounds, Mazzucotelli et al [132] separated one contrast agent and several degradation products using RP-HPLC/ICP-MS. Moreover, size exclusion chromatography (SEC) was used with ICP-MS to separate free Gd 3+ from Gd complexes and Gd adducts; however, the size differences between the individual Gd chelates are too small for effective SEC separation [133,134]. Ion chromatography (IC) [131,135], time-resolved luminescence (TRL) detector [136], and direct fluorescence [137] are also utilized for the separation of Gd species.…”

Section: Speciation Of Gd-casmentioning

confidence: 99%

Gadolinium as an Emerging Microcontaminant in Water Resources: Threats and Opportunities

Ebrahimi

Barbieri

2019

Geosciences

View full text Add to dashboard Cite

As a result of high doses of paramagnetic gadolinium (Gd) chelates administered in magnetic resonance imaging (MRI) exams, their unmetabolized excretion, and insufficient removal in wastewater treatment plants (WWTPs), large amounts of anthropogenic Gd (Gdanth) are released into surface water. The upward trend of gadolinium-based contrast agent (Gd-CA) administrations is expected to continue growing and consequently higher and higher anthropogenic Gd concentrations are annually recorded in water resources, which can pose a great threat to aquatic organisms and human beings. In addition, the feasibility of Gd retention in patients administered with Gd-CAs repeatedly, and even potentially fatal diseases, including nephrogenic systemic fibrosis (NSF), due to trace amounts of Gd have recently arisen severe health concerns. Thus, there is a need to investigate probable adverse health effects of currently marketed Gd-CAs meticulously and to modify the actual approach in using Gd contrast media in daily practice in order to minimize unknown possible health risks. Furthermore, the employment of enhanced wastewater treatment processes that are capable of removing the stable contrast agents, and the evaluation of the ecotoxicity of Gd chelates and human exposure to these emerging contaminants through dermal and ingestion pathways deserve more attention. On the other hand, point source releases of anthropogenic Gd into the aquatic environment presents the opportunity to assess surface water—groundwater interactions and trace the fate of wastewater plume as a proxy for the potential presence of other microcontaminants associated with treated wastewater in freshwater and marine systems.

show abstract

“…Upon analyzing the urine from a patient to whom a linear contrast agent (Gd-DTPA) had been administered, it was found that Gd-DTPA was excreted almost completely (>99 %) within one day. When applying SEC/ICP-MS in this investigation it turned out that no free Gd 3+ was present when compared to Gd-DTPA in the analyzed urine samples [137]. A CE/ESI-MS method was used to perform further investigations of possible transmetallation products (such as Fe-DTPA, Cu-DTPA, Zn-DTPA, and free DTPA) of Gd-DTPA in human blood samples.…”

Section: Metal-based Diagnosticsmentioning

confidence: 99%

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Meermann

Sperling

2012

Anal Bioanal Chem

View full text Add to dashboard Cite

Method development and applications of hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals are summarized within this review. Advantages and limitations of the separation modes-high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and gas chromatography (GC)-as well as the detection modes-inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization-mass spectrometry (ESI-MS)-are discussed. ICP-MS detection is found to be advantageous for the quantification of drugs containing metals and other heteroatoms. The species-independent sensitivity and multielement capabilities of ICP-MS allow it to be used for quantification even when species-specific standards are not available, as well as to determine the stoichiometry in metallodrug-biomolecule interactions. Molecular information that is totally destroyed when ICP is applied as ionization source and is therefore not obtainable via ICP-MS detection can be accessed by the complementary technique of ESI-MS. Speciation analysis combining both elemental and molecular information is therefore a powerful tool for the analysis of metal-based pharmaceuticals and their metabolites in body fluids and other relevant matrices.

show abstract

Determination of the MRI contrast agent Gd-DTPA by SEC?ICP?MS

Cited by 39 publications

References 11 publications

Serum-binding properties of isosteric ruthenium and osmium anticancer agents elucidated by SEC–ICP–MS

Serum-binding properties of isosteric ruthenium and osmium anticancer agents elucidated by SEC–ICP–MS

Gadolinium as an Emerging Microcontaminant in Water Resources: Threats and Opportunities

Hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals: developments and applications

Contact Info

Product

Resources

About