Determination of the Minimum Inhibitory Concentrations of Alexidine and Chlorhexidine Against Enterococcus faecalis and Candida albicans: An In Vitro Study

Basmacı, Fatma; Aksoy, Umut Mert; Kalender, Atakan; Öztan, Meltem Dartar; Oğuz, Ece İrem; Kıyan, Mehmet

doi:10.7759/cureus.2221

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…The sterilized substrate was added with 10 mL sterile water and used for actinobacterial inoculation. Thirdly, the strain J4 was used for inoculum preparation by dissolved with the sterile distilled water equivalent as McFarland No.0.5 (1.5 x 10 8 CFU/ml) that measured and had score between 0.08-0.1 by spectrophotometer at  625 nm and inoculated on the substrate prepared as mentioned above (Kermeoglu et al, 2018). 1 mL of the prepared J4 inoculum was added in the sterilized broken-milled rice and incubated at 30 o C for 7 days.…”

Section: Cultivation Of Actinobacteria On Brokenmilled Ricementioning

confidence: 99%

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The antibacterial activity and silk dyeing of the crude pigment extract from J4 actinobacteria strain

Urtgam,

Sumpradit,

Thurnkul

2023

JCST

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Actinobacteria represent a vast variety of prokaryotic microbes that produce a diverse group of secondary metabolites used in medical, agricultural, and industrial applications. The objectives in this study were to investigate the antibacterial activities and potential for silk dyeing of the crude extract from the actinobacterial strain J4. Initially, the antibacterial activities of the crude pigment extracts obtained from the actinobacterial strain J4 isolated from the wasp’s nest were determined. To extract the water-insoluble pigments used for antibacterial activity testing via the agar well diffusion method, ethyl acetate was applied during the pigment extraction. These extracts showed an inhibitory effect against Salmonella Typhimurium TISTR1472, Bacillus subtilis PSRU-01, and Enterococcus faecalis TISTR459. Secondly, evaluation of silk dyeing was carried out. We used the extractant as 70 % (v/v) ethanol in the alternative extraction process when the strain J4 was cultivated by solid-state fermentation with broken-milled rice to prepare actinobacterial dye for silk dyeing. The pigment extract obtained was tested for color stability based on washing methods, and the sensitivity to light was determined according to ISO105-C06:2010(E) and ISO 105-B02:2014(E), respectively. The color stability and sensitivity of the pigment extract to washing showed scores between 3-5, and the sensitivity to light demonstrated scores between 3-4. Taxonomically, the strain J4 was dark-violet colonies, and its water-soluble pigment diffused on sodium caseinate agar (SCA). To identify the strain J4, the phylogenetic identification based on partial sequencing of 16S rDNA was applied. The identification result indicated that the strain J4 was closely related to Streptomyces coacervatus (99.8% sequence similarity). In future, antibacterial activities of the crude extracts obtained from actinobacteria strain J4 should be tested with other food-borne pathogens. The development of Streptomyces dyes should also focus on the stability of the dyes during the silk dyeing process.

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Section: Cultivation Of Actinobacteria On Brokenmilled Ricementioning

confidence: 99%

“…The antibacterial activities of crude dye extract obtained from the J4 actinobacterial strain were determined by agar well diffusion method as described by Abraham, & Chauhan (2018) and Kermeoglu et al, (2018). The protocol was described as follows:…”

Section: Antibacterial Activity Testingmentioning

confidence: 99%

The antibacterial activity and silk dyeing of the crude pigment extract from J4 actinobacteria strain

Urtgam,

Sumpradit,

Thurnkul

2023

JCST

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“…A 0.0156% concentration of alexidine may be a good alternative to chlorhexidine as an irrigation solution in endodontic treatment when used for one minute against E. faecalis and C. albicans [42] . Also, probiotic organisms of Lactobacillus and Bifidobacterium species are suggested to be effective in preventing the growth of E. faecalis and C. albicans in vitro [43] .…”

Section: Treatmentmentioning

confidence: 99%

Candida albicans, from endodontic point of view

Perez¹,

Treviño-Tijerina²,

Leal-Camarillo³

et al. 2021

Int. J. Appl. Dent. Sci.

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Introduction: Candida albicans is a ubiquitous commensal organism that commonly colonizes the oral mucosa. In fact, up to 80% of the general population are carriers. Objective: To analyze the literature about C. albicans, its epidemiology, diagnosis, clinical manifestations, antimicrobial resistance and treatment in relation to endodontics. Methodology: Using the keywords Candida albicans in conjunction with epidemiology, diagnosis, clinical manifestations, antimicrobial resistance and treatment, the main public databases were searched, with emphasis on the last 5 years. It was evaluated with the PRISMA and AMSTAR-2 guidelines. Results: C. albicans is the fungus most frequently isolated in root canal infections, it binds to the dentin to form biofilms within the dentin tubules. C. albicans can be detected by different diagnostic methods, the most used is cultivation. This microorganism is increasingly found in the oral cavity, it is responsible for endodontic failure, its biofilms are inherently resistant to antifungal drugs, the host's immune system, environmental stresses and biomechanical treatment. The most favourable method to eliminate is using Qmi X irritant and Endo Vac as a suction system, together with chemo mechanical debridement. Conclusions: C. albicans is a pathogen which has been presenting itself more frequently in the oral cavity, in the endodontic area, it is the fungus that is most frequently found in the root canals, either due to primary or secondary infection.

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“…[10][11][12][13][14] Moreover, it may be an alternative to CHX due to its activity on E. faecalis and Candida albicans. 24 Adhesion of the root canal filling is one of the important clinical factor in endodontic treatment. 25 After disinfecting the root canals with irrigation solutions, a tight connection between the root canal filling and the root dentin contributes to the positive prognosis of root canal treatment.…”

Section: Introductionmentioning

confidence: 99%

Effect of Alexidine Dihydrochloride on the Bond Strength of Resin-Based Sealer to Dentin

Tezer¹,

Öztan²

2022

Atatürk Üniversitesi Diş Hekimliği Fakültesi Dergisi

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Bu çalışmanın amacı aralarında aleksidin dihidroklorürün (ALX) de bulunduğu farklı final irrigasyon solüsyonları kullanımı sonrası rezin esaslı kök kanal patının dentine bağlanma dayanımını değerlendirmektir.Yöntemler: 90 adet insan üst birinci keser dişi kullanıldı. Uygulanan son yıkama solüsyonuna göre gruplar şu şekilde oluşturuldu: Grup 1: 17% EDTA, Grup 2: QMix, Grup 3: %2 CHX, Grup 4: %1 ALX, Grup 5: Distile su (kontrol grubu). Tüm kanallar güta-perka ve AH Plus ile dolduruldu. Bağlanma dayanımı ölçümü için her bir örnekten koronalden apikale doğru 2 mm kalınlıkta üç yatay kesit elde edildi. Bağlantıda kopma oluşana kadar push-out kuvveti uygulandı. Bağlantı başarısızlığı, her bir örnek stereomikroskop ile değerlendirilerek şu şekilde sınıflandı: Adeziv (pat-dentin ya da pat-güta-perka arayüzünde), koheziv (pat ya da dentin arayüzünde), karma (hem pat hem dentinde). Gruplar arası karşılaştırmalar Kruskal-Wallis H testi ile yapıldı (P < .05).Bulgular: En yüksek ortalama bağlanma dayanımı değerleri sırasıyla koronal, orta ve apikal bölgede görüldü (P < .05). En yüksek ortalama bağlanma dayanımı değeri etilendiamin tetra-asetik asit (EDTA), en düşük kontrol grubundaydı (P < .001). Qmix'in, CHX ve ALX'le kıyaslandığında daha yüksek değer gösterdiği koronal bölge dışında, bu üç grup arasında farklılık yoktu. Tüm örneklerde en fazla koheziv başarısızlık görüldü.Sonuç: EDTA kullanıldığında kök kanalının her üç bölümünde de bağlanma dayanımı en yüksekti. ALX, CHX ile benzer bağlanma dayanımları gösterdi.

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Determination of the Minimum Inhibitory Concentrations of Alexidine and Chlorhexidine Against Enterococcus faecalis and Candida albicans: An In Vitro Study

Cited by 8 publications

References 28 publications

The antibacterial activity and silk dyeing of the crude pigment extract from J4 actinobacteria strain

The antibacterial activity and silk dyeing of the crude pigment extract from J4 actinobacteria strain

Candida albicans, from endodontic point of view

Effect of Alexidine Dihydrochloride on the Bond Strength of Resin-Based Sealer to Dentin

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