Determination of the Marker Diarylheptanoid Phytoestrogens in <i>Curcuma comosa</i> Rhizomes and Selected Herbal Medicinal Products by HPLC-DAD

Abstract

A method for quantification of diarylheptanoids in Curcuma comosa rhizomes and selected pharmaceutical preparations was established by using HPLC-diode array detector (DAD). The chromatographic separation of three diarylheptanoids [(3S)-1-(3,4-dihydroxy-phenyl)-7-phenyl-(6E)-6-hepten-3-ol (1), (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol (2), and (3S)-1,7-diphenyl-(6E)-6-hepten-3-ol (3)] was performed on a Luna C 18 analytical column using gradient elution with 0.5% acetic acid in water and acetonitrile with a flow rate of 1 mL/min and a column temperature of 35°C. The calibration curves for the analytes showed good linearity (R 2 >0.999), high precision (relative standard deviation (RSD) <2%) and acceptable recovery (98.35-103.90%, RSD <2%). The limit of detection (LOD) and limit of quantification (LOQ) were 0.06-0.22 and 0.18-0.69 µg/mL, respectively. The results of all validated parameters were within the limits according to the International Conference on Harmonization (ICH) Guidelines. The established method was successfully applied for qualitative and quantitative determination of the three constituents in different samples of C. comosa and some commercial products in capsules. The simplicity, rapidity, and reliability of the method could be useful for the fingerprint analysis and standardization of diarylheptanoids, which are responsible for the estrogenic activity in raw materials and herbal medicinal products of C. comosa.

Enhanced Stability and Bioactivity of Curcuma comosa Roxb. Extract in Electrospun Gelatin Nanofibers

“…10 mg of a gelatin nanofibrous mat loaded with CE was then re-dissolved in 2 mL of methanol. The content of diarylheptanoids in the solution mixture was determined using a UV-Vis spectrometer at 254 nm and 285 nm based on the method of Yingngam et al [10] and reported as mean value ± SD (n = 3).…”

“…The active constituents of C. comosa extract have been identified as acetophenones, curcuminoids, sesquiterpenes, and diarylheptanoids [9]. Additionally, the major bioactive compounds were diarylheptanoid derivatives at a level of 80-90% in crude extract [10]. Amongst non-phenolic diarylheptanoids, (3R)-1,7-diphenyl-(4E,6E)-4,6-heptadien-3-ol exhibited the highest anti-inflammatory [11], anti-oxidant [12,13] antibacterial activities [14] and anti-tyrosinase.…”