Quality and stability assessment of commercial products containing phytoestrogen diaryheptanoids from Curcuma comosa

Tabboon, Peera; Tuntiyasawasdikul, Sarunya; Sripanidkulchai, Bungorn

doi:10.1016/j.indcrop.2019.03.062

Cited by 7 publications

(5 citation statements)

References 27 publications

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“…However, it was surprising that the amount of OML and P7ME from different sources varied greatly. It is similar to a previous study [24], which suggested that the reason for the variation in the amount of active compounds is possibly due to inaccurate taxonomic assignments of cultivars. Toxicity or low effectiveness can occur if patients are subjected to too high or too low amounts of active ingredients of herbal formulations.…”

Section: Application To Samplesupporting

confidence: 91%

Development of the HPLC-DAD method for chromone determination and standardization in Harrisonia perforata (Blanco) Merr. and Thai herbal Ya-Ha-Rak formulation

Wechprakhon,

Sripanidkulchai,

Jaipakdee

et al. 2024

ScienceAsia

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Harrisonia perforata (Blanco) Merr. (H. perforata) is a plant material used in the Ya-Ha-Rak formula for the treatment of fever in COVID-19 patients in Thailand and contains two chromones as major active compounds, namely O-methylalloptaeroxylin (OML) and peucinin-7-methyl ether (P7ME). In Thailand, however, there is currently no standard quality control method available for this plant. This study aims to develop and validate analytical methods to measure levels of OML and P7ME in samples including branches, roots, and extracts of H. perforata, and Ya-Ha-Rak formulations. In the development process, a number of columns and elution conditions to determine OML and P7ME in the Ya-Ha-Rak formulations and H. perforata raw materials were investigated. Ideal conditions, including C18, highpurity silica at 4.6 × 150 mm, 3.5 µm particle size, controlling the column oven at 40 °C, and eluting with an acidic mobile phase, significantly improved the separation of OML and P7ME from other elutes in the chromatogram with good suitability parameters. The validation method gave good values for the correlation coefficient (R 2 = 0.9999) for both compounds. The limits of detection (LOD) and quantification (LOQ) were in the ranges of 1.31 and 1.03 µg/ml and 4.37 and 3.45 µg/ml, respectively. The recovery percentage fell between 99.97 and 109.75%, and the RSD value of inter-day and intra-day determinations was under 2.0%. The validated method was successfully applied to the analysis of real Ya-Ha-Rak and H. perforata raw material samples from various areas in Thailand.

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Section: Application To Samplesupporting

confidence: 91%

Development of the HPLC-DAD method for chromone determination and standardization in Harrisonia perforata (Blanco) Merr. and Thai herbal Ya-Ha-Rak formulation

Wechprakhon,

Sripanidkulchai,

Jaipakdee

et al. 2024

ScienceAsia

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“…Four formulations of C. comosa gels ( Table 5 ) were prepared, tightly sealed in an aluminum collapsible tube, then kept under accelerated and long-term conditions for 6 and 12 mo, respectively. C. comosa gels are considered to be drug products intended for storage in a refrigerator due to the degradation susceptibility of DAs to high-temperature storage [ 14 , 18 , 57 ]. Accordingly, the accelerated and long-term settings for the stability investigation of C. comosa gels were 5 ± 3 °C and 30 ± 2 °C/75 ± 5% RH, respectively (Climatic Zone IVb [ 40 ]).…”

Section: Resultsmentioning

confidence: 99%

“…The proposed degradation processes of DA1 and DA2 were oxidation and hydrogenation or dehydration. The increase in DA2 content upon storage under high temperatures, e.g., 40 °C, was associated with the degradation of some other components contained in C. comosa extract into DA2 [ 57 ]. This study confirms the findings of earlier research that the mucoadhesive gel of C. comosa extract was a product intended to be stored in a refrigerator or under low temperatures.…”

Section: Resultsmentioning

confidence: 99%

Formulation and In Vitro Evaluation of Mucoadhesive Sustained Release Gels of Phytoestrogen Diarylheptanoids from Curcuma comosa for Vaginal Delivery

et al. 2023

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Diarylheptanoids (DAs) characterized by a 1,7-diphenylheptane structural skeleton are considered a novel class of phytoestrogens. The DAs available in Curcuma comosa Roxb. (C. comosa) extract demonstrated significant estrogenic activities both in vitro and in vivo. This study aimed to develop and comprehensively evaluate a mucoadhesive vaginal gel for the sustained release of DAs. Different mucoadhesive polymers as gelling agents were investigated. C. comosa ethanolic crude extract was used as a source of DAs. All C. comosa gels were light brown homogeneous with pH within 4.4–4.6. Their flow behaviors were pseudoplastic with a flow behavior index of 0.18–0.38. The viscosity at a low shear rate varied from 6.2 to 335.4 Pa·s. Their mechanical and extrudability properties were associated well with rheological properties. Polycarbophil (PCP):hydroxypropyl methylcellulose (HPMC) blends had a higher mucoadhesiveness to porcine vaginal mucosa than those of PCP-based or HPMC-based gels. All C. comosa gels exhibited a sustained, zero-order DA release pattern over 72 h. Korsmeyer and Peppas equation fitting indicated a non-Fickian, case II transport release mechanism. C. comosa gels had good physical and chemical stability under low-temperature storage for up to 12 months. PCP:HPMC-based mucoadhesive gels could be a proper delivery system for vaginal administration of DAs.

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“…[51,52]). Products from rhizomes of C. comosa have been developed for use as an anti-inflammation remedy and for treatment of uterine abnormalities and ovarian hormone deficit [53,54]. [55]; b, Puangpairote [56]; c, Jenjittikul and Larsen [57]; d, Larsen [6]; e, Maknoi et al [49]; f, Boonma and Saensouk [48]; g, Chen et al [58]; and h, Puangpairote et al [59].…”

Section: The Genus Curcuma In Thailandmentioning

confidence: 99%

Polyploidy in the Ginger Family from Thailand

Anamthawat‐Jónsson¹,

Umpunjun²

2020

Chromosomal Abnormalities

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Polyploidy is common in the ginger family Zingiberaceae. The aims of the present paper are (1) to provide a general introduction on species diversity with emphasis on conservation; (2) to highlight the human-use significance of this family, focusing on the two major genera, Zingiber (ginger) and Curcuma (turmeric); (3) to present chromosome number data from 45 natural and cultivated Curcuma taxa from Thailand, of which polyploids are predominant; and (4) to describe our own work on cytotaxonomy of selected Thai Curcuma species. We obtained somatic chromosome numbers from root tips and analysed meiotic chromosome behaviour from flowers. We also used the molecular cytogenetic method of ribosomal gene mapping on chromosomes to infer mechanism of polyploidization and reveal genomic relationships among closely related species. The main results of our cytogenetic studies include the following. The most sought-after medicinal Curcuma cultivars growing on a large-scale basis are secondary triploids, so as taxa in natural habitats that are harvested for local utilisation. These triploids are sexually deficient, due to meiotic pairing abnormalities, but they are propagated asexually via rhizomes. The ribosomal mapping results indicate natural triploidization process via hybridisation, either within populations or across the species boundaries.

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Quality and stability assessment of commercial products containing phytoestrogen diaryheptanoids from Curcuma comosa

Cited by 7 publications

References 27 publications

Development of the HPLC-DAD method for chromone determination and standardization in Harrisonia perforata (Blanco) Merr. and Thai herbal Ya-Ha-Rak formulation

Development of the HPLC-DAD method for chromone determination and standardization in Harrisonia perforata (Blanco) Merr. and Thai herbal Ya-Ha-Rak formulation

Formulation and In Vitro Evaluation of Mucoadhesive Sustained Release Gels of Phytoestrogen Diarylheptanoids from Curcuma comosa for Vaginal Delivery

Polyploidy in the Ginger Family from Thailand

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