Determination of the Major Phenolic Compounds in Pomegranate Juices by HPLC–DAD–ESI-MS

Abstract

Traditionally, pomegranate (Punica granatum L.) has been consumed as fresh fruit or as pomegranate juice. In this study, the main phenolic compounds of 12 pomegranate varieties and 5 pomegranate clones were determined by HPLC−DAD−ESI-MS. Two chromatographic methods with a fused-core C18 column and a classical HPLC system were developed. Thirteen anthocyanins and fourteen other phenolic compounds were determined in the pomegranate juices. As far as we are concerned, a new flavonol-glycoside, phellatin or its isomer amurensin, has been tentatively identified for the first time in pomegranate juices. Total phenolic content ranged from 580.8 to 2551.3 mg/L of pomegranate juice. Anthocyanins varied between 20 to 82% of total phenolic content. Flavonoids were 1.6-23.6% of total phenolic compounds, while phenolic acids and ellagitannins were in the range 16.4-65.8%. The five clones reported a phenolic content comparable with that of the other pomegranate samples.

Characterization of chemical compositions and bioactive compounds in juices from pomegranates (‘Wonderful’, ‘Chaca’ and ‘Codpa’) at different maturity stages

“…This knowledge could allow for determination of the optimal harvest time for obtaining the maximum nutritional properties. Other studies on the chemical composition of fruits have also found significant variations in growing locations (Mphahlele et al, 2014;2016), phenolic compounds (Gil et al, 2000;Tzulker et al, 2007;Gómez-Caravaca et al, 2013;Fischer et al, 2013a;Li et al, 2015), sugars (Mphahlele et al, 2016), antioxidant activity (Borochov-Neori et al, 2009;Tehranifar et al, 2010;Zaouay et al, 2012), and mineral composition (Mirdehghan and Rahemi, 2007).…”

“…The glycosides of the three non-methylated anthocyanidins (delphinidin, cyanidin and pelargonidin) are the most abundant in nature. In this context, pomegranate is a rich source of 3-glucosides and 3,5-diglucosides of delphinidin, cyanidin and pelargonidin (Gómez-Caravaca et al, 2013;Radunic et al, 2015).…”

A New HPLC-PAD/HPLC-ESI-MS Method for the Analysis of Phytoestrogens Produced by Bacterial Metabolism

“…Several methods were considered for the analysis of the phytoestrogen metabolism by bacteria (Heinonen et al 2001;Cerdá et al 2006;Possemiers et al 2007;Ito et al 2008;Dueñas et al 2009;Bartkiene et al 2011;Elghali et al 2012;Gómez-Caravaca et al 2013). Given the difficulty of finding standards for all isoflavones, lignans, and ellagitannins, as well as the high price of most of them, only standards of the most interesting phytoestrogens were acquired in the present work.…”

“…To date, several chromatographic methods have been developed to detect the presence of phytoestrogens in foods (Heinonen et al 2001;Cerdá et al 2006;Possemiers et al 2007;Ito et al 2008;Bartkiene et al 2011;Elghali et al 2012;Gómez-Caravaca et al 2013). However, none allowed isoflavones, lignans, and ellagitannins to be simultaneously analyzed.…”

Ameliorative Effects of Pomegranate Peel Extract on Hepatotoxicity Induced by Carbon Tetrachloride in Mice

“…A number of biological activities have been reported on various extracts/constituents of different parts of this plant such as, antibacterial [4] antifungal [5], antiulcer [6] and antitumour [7]. Recently, the antioxidant activity of pomegranate is obvious among in pomegranate fruit, juice and peel [8], this is due to that Pomegranate contains large amounts of polyphenols and flavonoid [9]. Pomegranate peel extracts (PPE) with large quantity of flavonoids and tannins have been shown a high antioxidant activity in several studies [10].…”