Determination of the major mercapturic acids of 1,3-butadiene in human and rat urine using liquid chromatography with tandem mass spectrometry

Urban, Michael; Gilch, Gerhard; Schepers, Georg; Miert, E. Van; Scherer, Gerhard

doi:10.1016/j.jchromb.2003.08.009

Cited by 64 publications

(66 citation statements)

References 11 publications

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“…Although numerous methods for the quantification of different MAs by LC-MS/MS have been reported in the recent past, the majority of those assays target a rather small set of up to six MAs [8,17,[20][21][22][24][25][26]. In addition, many of these methods lack the capability of quantifying MAs from environmental exposures in low levels.…”

Section: Discussionmentioning

confidence: 99%

Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods

et al. 2015

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Mercapturic acids (MAs) are metabolic end products formed from conjugates between glutathione and electrophilic compounds. MAs are, therefore, suitable biomarkers of exposure to toxicants, which are either electrophiles by themselves or metabolized to electrophilic intermediates. We developed and validated two LC-MS/MS methods which allow the complementary, rapid, and sensitive determination of MAs derived from acrolein, acrylamide, acrylonitrile, benzene, 1,3-butadiene, crotonaldehyde, N,N-dimethylformamide, ethylene, ethylene oxide, vinyl chloride, propylene oxide, styrene, toluene as well as methylating and ethylating agents. Since separate determinations of single or small groups of MAs are time-consuming and expensive, we multiplexed several individual methods into two LC-MS/MS methods covering 18 individual mercapturic acids. Method validation according to FDA guidelines showed excellent results in terms of robustness, accuracy, and sensitivity of the methods. Moreover, the use of a minimal, simple, and straightforward sample cleanup procedure further accelerated the analytical workflow, which allows a time- and cost-efficient analysis of up to 18 MAs derived from various toxicants in environmental levels. The methods were applied to urine samples derived from a strictly diet-controlled clinical study, including 25 smoking and 25 non-smoking subjects. Significant increase in the urine concentrations in smokers as compared to non-smokers (p < 0.01; Student t test) was observed for 13 individual MAs. Moreover, a dose dependence was obtained for the majority of the analytes. In conclusion, the newly developed assays represent a powerful tool for the fast and reliable quantification of 18 MAs in clinical studies. A first method application suggests several suitable biomarkers for nine relevant toxicants in tobacco smoke.

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Section: Discussionmentioning

confidence: 99%

Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods

et al. 2015

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“…Urban et al achieved excellent results in the detection of two mercapturic acids of 1,3-butadiene and several other mercapturic acids applying atmospheric pressure chemical ionisation (APCI) [32] with limits of detection (LOD) in the lower g/L range. We therefore tested the APCI-source and compared it to electrospray ionisation (ESI) on the API 2000.…”

Section: Mass Spectrometrymentioning

confidence: 99%

Determination of the major mercapturic acids of acrylamide and glycidamide in human urine by LC–ESI-MS/MS

Boettcher

Angerer

2005

Journal of Chromatography B

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“…It is therefore difficult to retain these substances on traditional reversed phases (RP). In previous works chromatographic separation of mercapturic acids was predominantly achieved by using more polar RP-columns like "polar embedded" stationary phases or stationary phases with shorter chain lengths (C8-columns) [9,17,20,24,27,33]. Separation on these columns was mostly realized by using high amounts of aqueous mobile phase and a low amount of organic solvents.…”

Section: Chromatographymentioning

confidence: 99%

“…Metabolism of butadiene leads to the excretion of two mercapturic acids in urine: 3,4-dihydroxybutyl mercapturic acid (DHBMA) and monohydroxy-3-butenyl mercapturic acid (MHBMA), whereas the latter consists of two isomeric forms (see Fig. 1) [21][22][23][24][25]. In human urine DHBMA is usually found in higher concentrations than MHBMA [26,27].…”

Section: Introductionmentioning

confidence: 99%

Determination of six hydroxyalkyl mercapturic acids in human urine using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC–ESI-MS/MS)

Eckert

Drexler

Göen

2010

Journal of Chromatography B

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Determination of the major mercapturic acids of 1,3-butadiene in human and rat urine using liquid chromatography with tandem mass spectrometry

Cited by 64 publications

References 11 publications

Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods

Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods

Determination of the major mercapturic acids of acrylamide and glycidamide in human urine by LC–ESI-MS/MS

Determination of six hydroxyalkyl mercapturic acids in human urine using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC–ESI-MS/MS)

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