Determination of six hydroxyalkyl mercapturic acids in human urine using hydrophilic interaction liquid chromatography with tandem mass spectrometry (HILIC–ESI-MS/MS)

Eckert, Elisabeth; Drexler, Hans; Göen, Thomas

doi:10.1016/j.jchromb.2009.09.003

Cited by 55 publications

(43 citation statements)

References 35 publications

(55 reference statements)

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“…3-HPMA (3-hydroxypropyl mercapturic acid) in urine was measured by liquid chromatography with tandem mass spectrometry (LC-MS/MS) [44]. As shown in Fig.…”

Section: Distinction Between Control Subjects and MCI And Ad Patientmentioning

confidence: 99%

Evaluation of dementia by acrolein, amyloid-β and creatinine

Igarashi

Yoshida

Waragai

et al. 2015

Clinica Chimica Acta

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“…3-HPMA (3-hydroxypropyl mercapturic acid) in urine was measured by liquid chromatography with tandem mass spectrometry (LC-MS/MS) [44]. As shown in Fig.…”

Section: Distinction Between Control Subjects and MCI And Ad Patientmentioning

confidence: 99%

Evaluation of dementia by acrolein, amyloid-β and creatinine

Igarashi

Yoshida

Waragai

et al. 2015

Clinica Chimica Acta

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“…3-Hydroxypropyl mercapturic acid (3-HPMA) was analyzed in urine according to Eckert et al 16 Solid phase extraction with Isolute ENV + cartridges (Biotage, Charlotte, NC) was used to prepare each sample before LC/MS/MS analysis. Each cartridge was conditioned with 1 mL of methanol, followed by 1 mL of water, and then 1 mL of 0.1% formic acid in water.…”

Section: -Hydroxypropyl Mercapturic Acid Analysis In Urinementioning

confidence: 99%

Determination of Urine 3-HPMA, a Stable Acrolein Metabolite in a Rat Model of Spinal Cord Injury

Zheng

Park

Walls

et al. 2013

Journal of Neurotrauma

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Acrolein has been suggested to be involved in a variety of pathological conditions. The monitoring of acrolein is of significant importance in delineating the pathogenesis of various diseases. Aimed at overcoming the reactivity and volatility of acrolein, we describe a specific and stable metabolite of acrolein in urine, N-acetyl-S-3-hydroxypropylcysteine (3-HPMA), as a potential surrogate marker for acrolein quantification. Using the LC/MS/MS method, we demonstrated that 3-HPMA was significantly elevated in a dose-dependent manner when acrolein was injected into rats IP or directly into the spinal cord, but not when acrolein scavengers were co-incubated with acrolein solution. A nonlinear mathematic relationship is established between acrolein injected directly into the spinal cord and a correlated dosedependent increase of 3-HPMA, suggesting the increase of 3-HPMA becomes less apparent as the level of injected acrolein increases. The elevation of 3-HPMA was further detected in the rat spinal cord injury, a pathological condition known to be associated with elevated endogenous acrolein. This finding was further validated by concomitant confirmation of increased acrolein-lysine adducts using established dot immunoblotting techniques. The noninvasive nature of measuring 3-HPMA concentrations in urine allows for long-term monitoring of acrolein in the same animal and ultimately in human clinical studies. Due to wide spread involvement of acrolein in human health, the benefits of this study have the potential to enhance human health significantly.

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“…However, the formation of the chlorine containing mercapturic acid CHPMA is known to be highly favored [11][12][13]. Recently, we have already developed a suitable biomonitoring method for several mercapturic acids that includes the determination of DHPMA [23]. The application of this method to urine samples of several occupationally non-exposed individuals revealed a comparatively high urinary background level of DHPMA, which could not be linked to a corresponding exposure to glycidol or epichlorohydrin [24].…”

Section: Selection Of Biomarkersmentioning

confidence: 99%