Determination of the Isoelectric Point of Bovine Plasma Albumin by Cellulose Acetate Paper Electrophoresis

Stabilization of Magnetic Iron Oxide Nanoparticles in Biological Media by Fetal Bovine Serum (FBS)

“…The acidic surface interacts strongly with basic amino groups of the protein molecules, resulting in the chemical adsorption of the protein onto the particle surface . The fact that most of the proteins that remain strongly attached to the MNP surface after three cycles of washing with Milli-Q water have an isoelectric point (pI) of 5.0 or more − (Table ) further supports the postulation that the strong attachment of protein to the MNP surface is caused by this mechanism. This result also concurs with the study carried out by Gessner et al, which showed that proteins with a pI of greater than 5.5 prefer to bind to nanoparticles with an acidic surface whereas proteins with a pI of less than 5.5 are more likely to bind to latex nanoparticles with basic surfaces after the particles have been in contact with human serum for 5 min …”

Some Properties of an Alkaline Protease Inhibitor, AP-I, and Its Effect on α-Chymotrypsin and Trypsin

“…The isoelectric point determined by the method of cellulose acetate electrophoresis 6 ) was pH 4.0. Absorbance of 1 % AP-I solution at 278 nm (£~t"m) was 7.8.…”

Electrophoresis

“…The genetics of serum protein polymorphism in cattle has been investigated (436,556,1138). A new «-globulin has been detected in calf fetus (684)• The isoelectric point of bovine albumin varies between 4.3 and 4.6 depending upon the ionic strength of the electrophoretic buffer (724)• The serum electrophoretic patterns of moose, mountain goat, sheep, elk, and reindeer, including transferrin patterns, have been studied (909,911); sheep (718) and sheep and goats (1139) have many transferrin phenotypes. Serum proteins of ewes vary in relation to pregnancy (570) and lactation (419).…”