Some Properties of an Alkaline Protease Inhibitor, AP-I, and Its Effect on α-Chymotrypsin and Trypsin

Properties of 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase in Villous and Crypt Cells of the Rat Small Intestine

“…5 ) Thus, reductase activity in the subcellular fraction of villi could be measured only when trypsin inhibitor from soybean (or from egg white) was present in the fractionation media and perhaps in the assay media. Other types of protease inhibitor such as tosyl phenylalanine chloromethylketone, monobenzyl epoxysuccinate, leupeptin, pepstatin N) or fungal alkaline protease inhibitor 15 ) and toluene sulfonylfluoride (unpublished data) were all not effective for measurement of the reductase in the subcellular fractions of villi. The reductase activity in microsomes of crypt cells could be measured without addition of trypsin inhibitor.…”

Streptomyces lavendulaeProtease Inhibitor: Purification, Gene Overexpression, and 3-Dimensional Structure

“…Weight. Protease inhibitor from S. griseoincarnatus [32,33]. Protein protease inhibitors have been classified based on their size, structure, and disulfide 3 bond arrangement [34].…”

API-2b, a New Alkaline Protease Inhibitor Produced byStreptomyces griseoincarnatusStrain No. KTo-250

“…KTo-332, and purification, crystallization and some properties of this inhibitor were also described. 2 ) Though AP-I differed from S-SI with respects to the behavior on EI complex formationS) and no inhibition of a-chymotrypsin and trypsin in the case of p-nitrophenyl acetate as substrate,2) this inhibitor seems to have similar characteristics as S-SLh6) To obtain new inhibitors, further screening tests were carried out and a new alkaline protease inhibitor was found to be produced in the culture filtrate of Streptomyces griseoincarnatus strain No. KTo-250.…”