Determination of the glycosylation-pattern of the middle ear mucosa in guinea pigs

Engleder, Elisabeth; Demmerer, Elisabeth; Wang, Xueyan; Honeder, Clemens; Zhu, Chengjing; Studenik, Christian; Wirth, Michael; Arnoldner, Christoph; Gabor, Franz

doi:10.1016/j.ijpharm.2015.02.056

Cited by 4 publications

(4 citation statements)

References 38 publications

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“…Carbohydrate structures present on diseased cells participate in carbohydrate–lectin interactions and help in signalling processes. The wheat germ agglutinin (WGA) was found to be the most bioadhesive of all the lectins [ 56 ]. The lectin receptor SIGN-R1 is used by DCs in the lymph node medulla to collect lymph-borne influenza virus and to activate humoral response [ 57 ].…”

Section: Animal Lectins As Receptors For Receptor-mediated Targetingmentioning

confidence: 99%

Applications of mannose-binding lectins and mannan glycoconjugates in nanomedicine

Gupta

2022

J Nanopart Res

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Glycosylated nanoparticles (NPs) have drawn a lot of attention in the biomedical field over the past few decades, particularly in applications like targeted drug delivery. Mannosylated NPs and mannan-binding lectins/proteins (MBL/MBP) are emerging as promising tools for delivery of drugs, medicines, and enzymes to targeted tissues and cells as nanocarriers, enhancing their therapeutic benefits while avoiding the adverse effects of the drug. The occurrence of plenty of lectin receptors and their mannan ligands on cell surfaces makes them multifaceted carriers appropriate for specific delivery of bioactive drug materials to their targeted sites. Thus, the present review describes the tethering of mannose (Man) to several nanostructures, like micelles, liposomes, and other NPs, applicable for drug delivery systems. Bioadhesion through MBL-like receptors on cells has involvements applicable to additional arenas of science, for example gene delivery, tissue engineering, biomaterials, and nanotechnology. This review also focuses on the role of various aspects of drug/antigen delivery using (i) mannosylated NPs, (ii) mannosylated lectins, (iii) amphiphilic glycopolymer NPs, and (iv) natural mannan-containing polysaccharides, with most significant applications of MBL-based NPs as multivalent scaffolds, using different strategies. Graphical abstract Mannosylated NPs and/or MBL/MBP are coming up as viable and versatile tools as nanocarriers to deliver drugs and enzymes precisely to their target tissues or cells. The presence of abundant number of lectin receptors and their mannan ligands on cell surfaces makes them versatile carriers suitable for the targeted delivery of bioactive drugs.

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Section: Animal Lectins As Receptors For Receptor-mediated Targetingmentioning

confidence: 99%

Applications of mannose-binding lectins and mannan glycoconjugates in nanomedicine

Gupta

2022

J Nanopart Res

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“…The lectin working solutions were prepared by diluting the stock solutions with simulated nasal electrolyte solution (SNES) to a final lectin concentration of 500 nmol/L. The lectin concentration was chosen based on a study by Engleder, Demmerer, Wang, Honeder, Zhu, Studenik, Wirth, Arnoldner, and Gabor [ 16 ] in a guinea pig model. The SNES contained 7.45 mg/mL of sodium chloride, 1.29 mg/mL of potassium chloride, and 0.32 mg/mL of calcium chloride dihydrate in distilled water [ 30 ].…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, knowledge about the tissue-specific carbohydrate composition and alterations under pathologic conditions is crucial for the development of lectin-mediated glycotargeting strategies like lectin-functionalised drug molecules [ 10 , 11 ] and drug delivery systems [ 12 , 13 , 14 , 15 ]. The concept of plant lectin-based targeting was already investigated in the past for several different tissue types, such as the middle ear mucosa [ 16 ], the urothelium [ 17 ], and the intestinal epithelium [ 18 ], with the aim of serving as a basis for bioadhesive drug targeting. Bioadhesive nasal drug delivery systems have been shown to increase residence time at the nasal mucosa by avoiding rapid clearance [ 19 ], which is crucial for successful intranasal drug delivery.…”

Section: Introductionmentioning

confidence: 99%

A Novel Preparation Technique for Human Nasal Respiratory Mucosa to Disclose Its Glycosylation Pattern for Bioadhesive Drug Delivery

et al. 2023

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To shed some light on glycotargeting as a potential strategy for nasal drug delivery, a reliable preparation method for human nasal mucosa samples and a tool to investigate the carbohydrate building blocks of the glycocalyx of the respiratory epithelium are required. Applying a simple experimental setup in a 96-well plate format together with a panel of six fluorescein-labeled lectins with different carbohydrate specificities allowed for the detection and quantification of accessible carbohydrates in the mucosa. As confirmed by binding experiments at 4 °C, both quantitatively by fluorimetry and qualitatively by microscopy, the binding of wheat germ agglutinin exceeded that of the others by 150% on average, indicating a high content of N-acetyl-D-glucosamine and sialic acid. Providing energy by raising the temperature to 37 °C revealed uptake of the carbohydrate-bound lectin into the cell. Moreover, repeated washing steps during the assay gave a slight hint as to the influence of mucus renewal on bioadhesive drug delivery. All in all, the experimental setup reported here for the first time is not only a suitable approach to estimating the basics and potential of nasal lectin-mediated drug delivery but also meets the needs for answering a broad variety of scientific questions involving the use of ex vivo tissue samples.

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“…Six studies to date have investigated OM-associated changes in glycosylation pattern or cell surface carbohydrates using lectins in ME and ET [ 74 , 75 , 76 , 77 , 78 , 79 ]. These studies utilized 5–7 types of fluorescein-labeled lectin.…”

Section: Types and Functions Of Clrs Expressed In The Middle Ear Amentioning

confidence: 99%

Expression, Distribution, and Role of C-Type Lectin Receptors in the Human and Animal Middle Ear and Eustachian Tube: A Review

Jung

Kim

et al. 2018

Molecules

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Otitis media (OM) is a group of inflammatory diseases of the middle ear (ME), regardless of cause or pathological mechanism. Among the molecular biological studies assessing the pathology of OM are investigations into the expression of C-type lectin receptors (CLR) in the ME and Eustachian tube (ET). To date, nine studies have evaluated CLR expression in the ME and ET. The expression of individual CLRs in mammalian ME and ET varies by species and model of OM. Assessments have shown that the patterns of CLR expression in the ME and ET vary; that CLR expression may vary by type of OM; and that the distribution and levels of expression of CLRs may depend on the presence or absence of inflammation, with variations even within the same species and same tissue. Infection of the ME and ET with various pathogens is a common cause of all types of OM, with host responses to pathogens mediated initially by the innate immune system. CLRs are important factors in the innate immune system because they act as both adhesion molecules and as pathogen recognition receptors. The expression of CLRs in OM tissues suggests that CLRs are associated with the pathogenesis of various types of OM.

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Determination of the glycosylation-pattern of the middle ear mucosa in guinea pigs

Abstract: Graphical abstract

Cited by 4 publications

References 38 publications

Applications of mannose-binding lectins and mannan glycoconjugates in nanomedicine

Applications of mannose-binding lectins and mannan glycoconjugates in nanomedicine

A Novel Preparation Technique for Human Nasal Respiratory Mucosa to Disclose Its Glycosylation Pattern for Bioadhesive Drug Delivery

Expression, Distribution, and Role of C-Type Lectin Receptors in the Human and Animal Middle Ear and Eustachian Tube: A Review

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