Determination of the genotoxic effects of Convolvulus arvensis extracts on corn (Zea mays L.) seeds

Abstract

In this research, the methanolic extracts of Convolvulus arvensis were tested for genotoxic and inhibitor activity on the total soluble protein content and the genomic template stability against corn Zea mays L. seed. The methanol extracts of leaf, stem and root of C. arvensis were diluted to 50, 75 and 100 μl concentrations and applied to corn seed. The total soluble protein and genomic template stability results were compared with the control. The results showed that especially 100 μl extracts of diluted leaf, stem and root had a strong inhibitory activity on the genomic template stability. The changes occurred in random amplification of polymorphic DNA (RAPD) profiles of C. arvensis extract treatment included variation in band intensity, loss of bands and appearance of new bands compared with control. Also, the results obtained from this study revealed that the increase in the concentrations of C. arvensis extract increased the total soluble protein content in maize. The results suggested that RAPD analysis and total protein analysis could be applied as a suitable biomarker assay for the detection of genotoxic effects of plant allelochemicals.

Growth and Molecular Expression of Okra Seeds Interacted with Fourteen Mango Cultivars in Mixed Cropping System

“…These allelochemicals could play an important role in inhibiting enzymes involved in these two processes accordance with Baziramakenga et al (1997) who pointed out that the methionine incorporation into proteins was reduced by allelochemicals. Furthermore, it is notable the intensity of protein bands decrease or increase depended on type of allelochemicals found in the fourteen mango cultivars, which affected on the recipient species (H. esculentus) (El-Khawas & Shehata, 2005;Sunar et al, 2013;Yumnamcha et al, 2014 ).…”

“…This interference took place either by induction or repression of the protein bands. The relatively high frequency of disappearance of bands may reveal that the survival of the individuals was greatly affected by allelochemicals (El-Khawas and Shehata, 2005;Cenkci et al, 2010;Sunar et al, 2013).…”

Allelopathic Effect of Convolvulus arvensis L. Extracts on The Phytohormones and Cytological Processes of Zea mays L. Seeds

“…The statistically significant increase was determined in SA level depending on the various concentration of leaf and root extract for corn seed ( Table 2 and Figure 2) [21]. The lowest level of IAA was found in the treatment of 100 µl leaf extract for corn.…”

“…In this situation, it is possible to say that ABA and SA synthesis increases to reduce oxidative damage in plants. It has been well known that exogenic ABA lead to synthesis of some new protein types, inhibition of some proteins and also increase and decrease of synthesis of some proteins which synthesize under unstressed conditions in plant cells [21,23]. It means that ABA may play a role change of the gene expression model in plants.…”

Evaluation of Genotoxic and Inhibitory Effects of Invasive Weed Wild Poinsettia (Euphorbia heterophylla Linn.) in Sunflower

“…There is no common mode of action or physiological target site for all allelochemicals (Kato-Noguchi et al, 2002) even though some plant extracts are known to have allelopathic effects on cell division, pollen germination (Turker et al, 2008), nutrient uptake, photosynthesis, specific enzyme function (Han et al, 2008), genomic template stability (GTS) and total soluble protein content of the seedlings (Sunar et al, 2012).…”