Determination of the GABAB receptor agonist CGP 44532 (3-amino-2-hydroxypropylmethylphosphinic acid) in rat plasma after pre-column derivatization by micro-high-performance liquid chromatography combined with negative electrospray tandem mass spectrometry

Sensitivity enhancement in liquid chromatography/atmospheric pressure ionization mass spectrometry using derivatization and mobile phase additives

“…The limit of detection was found to be 0.2-3 fmol for several diamines in the negative ESI detection mode. 4-Nitrobenzylchloroformate was used to form carbamate ester derivative with primary amines; the LOQ was found to be 10 ng/mL using negative ESI [48]. The sensitivity of detection for amines has been improved using EC-NAPCI after derivatization with PFB-Br [29].…”

Amino acid analysis using chromatography–mass spectrometry: An inter platform comparison study

“…chloroformate derivatives of AAs, which were used in GC-MS, can also be analyzed by RPLC-MS/MS[43]. For application in LC-MS, the sample preparation is identical to that of GC-MS analysis, except for sample reconstitution before analysis.Ammonium formate in aqueous methanol is used as a reconstitution solvent instead of the mixture of isooctane and chloroform.…”