Amino acid analysis using chromatography–mass spectrometry: An inter platform comparison study

Krumpochova, Petra; Bruyneel, Ben; Molenaar, Douwe; Koukou, A.; Wuhrer, Manfred; Niessen, W.M.A.; Giera, Martin

doi:10.1016/j.jpba.2015.06.001

Cited by 66 publications

(44 citation statements)

References 46 publications

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“…RP-HPLC and GC typically require chemical derivatization prior to detection, although newer HPLC column chemistries are facilitating improved retention characteristics and enable baseline separations of non-derivatized amino acids, limiting biases and imprecision associated with a derivatization step (47). High resolution amino acid separations are also possible using capillary electrophoresis (48).…”

Section: Quantifying Pure Peptides By Amino Acid Analysismentioning

confidence: 99%

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays

et al. 2016

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BACKGROUND For many years, basic and clinical researchers have taken advantage of the analytical sensitivity and specificity afforded by mass spectrometry in the measurement of proteins. Clinical laboratories are now beginning to deploy these work flows as well. For assays that use proteolysis to generate peptides for protein quantification and characterization, synthetic stable isotope–labeled internal standard peptides are of central importance. No general recommendations are currently available surrounding the use of peptides in protein mass spectrometric assays. CONTENT The Clinical Proteomic Tumor Analysis Consortium of the National Cancer Institute has collaborated with clinical laboratorians, peptide manufacturers, metrologists, representatives of the pharmaceutical industry, and other professionals to develop a consensus set of recommendations for peptide procurement, characterization, storage, and handling, as well as approaches to the interpretation of the data generated by mass spectrometric protein assays. Additionally, the importance of carefully characterized reference materials—in particular, peptide standards for the improved concordance of amino acid analysis methods across the industry—is highlighted. The alignment of practices around the use of peptides and the transparency of sample preparation protocols should allow for the harmonization of peptide and protein quantification in research and clinical care.

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Section: Quantifying Pure Peptides By Amino Acid Analysismentioning

confidence: 99%

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays

et al. 2016

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“…Nevertheless, GC shows advances particularly in terms of separation efficiency. This superior separation efficiency is explained by the absence of eddy-diffusion during the chromatographic separation compared with LC [59].…”

Section: Chromatographic and Electrophoretic Techniquesmentioning

confidence: 99%

“…A possible alternative to silylation reagents may be the use of chloroformate or methylation reagents [59,67]. Some of the aforementioned pitfalls were addressed by Villas Boas et al, who investigated compound methylation using methylchloroformate as an alternative derivatization strategy [69].…”

Section: Sample Preparationmentioning

confidence: 99%

Analytical Pitfalls and Challenges in Clinical Metabolomics

et al. 2016

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Metabolomics-based strategies have become an integral part of modern clinical research, allowing for a better understanding of pathophysiological conditions and disease mechanisms, as well as providing innovative tools for more adequate diagnostic and prognosis approaches. Metabolomics is considered an essential tool in precision medicine, which aims for personalized prevention and tailor-made treatments. Nevertheless, multiple pitfalls may be encountered in clinical metabolomics during the entire workflow, hampering the quality of the data and, thus, the biological interpretation. This review describes the challenges underlying metabolomics-based experiments, discussing step by step the potential pitfalls of the analytical process, including study design, sample collection, storage, as well as preparation, chromatographic and electrophoretic separation, detection and data analysis. Moreover, it offers practical solutions and strategies to tackle these challenges, ensuring the generation of high-quality data.

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“…Thus, EAPPI is more favorable than gas chromatography (GC)-MS for the qualitative analysis of amino acids. According to the results of previous publications [28,29], derivatization is mandatory for GC-based analysis of amino acids and a relatively longer time (>8 min) was needed than EAPPI analysis (within 1 min). Figure 3 shows the EAPPI mass spectra of tangerine (Citrus reticulata) and grapefruit (Citrus paradise) peels after UNE treatment.…”

Section: ) and This Shows [M + H -H 2 O] + For L-threonine And [M + H]mentioning

confidence: 99%

Extractive Atmospheric Pressure Photoionization (EAPPI) Mass Spectrometry: Rapid Analysis of Chemicals in Complex Matrices

Liu

Yang

Jian

et al. 2016

J. Am. Soc. Mass Spectrom.

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Abstract. Extractive atmospheric pressure photoionization (EAPPI) mass spectrometry was designed for rapid qualitative and quantitative analysis of chemicals in complex matrices. In this method, an ultrasonic nebulization system was applied to sample extraction, nebulization, and vaporization. Mixed with a gaseous dopant, vaporized analytes were ionized through ambient photon-induced ion-molecule reactions, and were mass-analyzed by a high resolution time-of-flight mass spectrometer (TOF-MS). After careful optimization and testing with pure sample solution, EAPPI was successfully applied to the fast screening of capsules, soil, natural products, and viscous compounds. Analysis was completed within a few seconds without the need for preseparation. Moreover, the quantification capability of EAPPI for matrices was evaluated by analyzing six polycyclic aromatic hydrocarbons (PAHs) in soil. The correlation coefficients (R 2 ) for standard curves of all six PAHs were above 0.99, and the detection limits were in the range of 0.16-0.34 ng/mg. In addition, EAPPI could also be used to monitor organic chemical reactions in real time.

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Amino acid analysis using chromatography–mass spectrometry: An inter platform comparison study

Cited by 66 publications

References 46 publications

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays

Recommendations for the Generation, Quantification, Storage, and Handling of Peptides Used for Mass Spectrometry–Based Assays

Analytical Pitfalls and Challenges in Clinical Metabolomics

Extractive Atmospheric Pressure Photoionization (EAPPI) Mass Spectrometry: Rapid Analysis of Chemicals in Complex Matrices

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