Although current proposed water recovery systems in space do not incorporate biotreatment technologies, research has indicated that bioprocessors (BP) may be a potential primary treatment technology in integrated advanced life support systems for long-duration space missions. Little data have been reported on the activity of nonacidified wastewater in the collection system prior to entering water recovery systems. These reactions are vital in accurately quantifying biosystem performance, mass balances, and microbial kinetics for bioprocessors operated on dynamic wastestreams. The bioavailable dissolved organic carbon and ammonium concentrations of storage tank grab samples may vary by up to 44 ± 9% and 81 ± 12%, respectively, in a urine-containing wastestream. A model for quantifying the average rate of microbially based holding tank activity is presented. A brief enzymatic inhibition study indicated that surfactants contained within the wastestream may be inhibiting feed tank urease activity, causing the organic carbon and ammonium variability. The aim of this research is to quantify storage tank reactions so that future bioprocessor models reflect only the activity occurring within the BP, and not that of the feed tank. Although microbially active collection vessels may be a consideration for long-duration missions, their activity should be modeled independently to avoid the "black box" approach to modeling BP dynamics. The results will aid future researchers in operating biosystems on urine-gray water wastestreams.