Determination of the expression levels of DNA methyltransferase genes during a highly efficient regeneration system via shoot organogenesis in the diploid apomict Boechera divaricarpa

Taşkın, Kemal Melih; Özbilen, Aslıhan; Sezer, Fatih; Çördük, Nurşen; Erden, Damla

doi:10.1007/s11240-014-0704-0

Cited by 6 publications

(8 citation statements)

References 67 publications

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“…These results were in contrast with Taskin and his co-workers' results who indicated that the expression of CMT3 was high in Boechera divarica. CMT3 played the main part in challenging the stress conditions through shoot regeneration (Taskin et al, 2015). DRM2 expression was unchanged in the three media (0.4, 0.5, and 0.5 folds, respectively except with medium 1 (TDZ & IBA) (0.9 fold) which has a slight increase than the other media (control, medium 2, and medium 3).…”

Section: Dna Methylationmentioning

confidence: 87%

“…It has been suggested that regenerated plants have different DNA methylation and histone modifications profiles. It can be regarded as epigenetic changes which might be connected with somatic variations (Taskin et al, 2015).…”

Section: Chromatin Modificationsmentioning

confidence: 99%

“…The conditions of tissue culture cause changes in epigenetic modification through methylation of DNA (Taskin et al, 2015). The levels of DNA methylation genes are highly influenced by applied PGRs in tissue culture (Huang et al, 2012;Taskin et al, 2015).…”

Section: Dna Methylationmentioning

confidence: 99%

“…In tissue culture and regeneration systems, plant growth regulators (PGRs) might cause stress and epigenetic variations in regenerated plants (Chinnusamy and Zhu, 2009). To overcome this stress, DNA methylation can be utilized for alterations of epigenetic and gene regulations (Taskin et al, 2015). Some epigenetic changes (methylation of DNA, methylation & acetylation of histones) take place for gene expression regulations (Zhang & Reinberg, 2001;Wójcikowska, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

Ismail

Ali

Nagaty

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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Establishment of an efficient adventitious shoot regeneration system for mature embryo of Prunus persica via shoot organogenesis has been achieved. Three different combinations of plant growth regulators (PGRs) have been applied in the current study; 6-Benzyl adenine (BA) or thidiazuron (TDZ) with Indole acetic acid (IAA) or Indole butyric acid (IBA). The high regeneration frequency was illustrated by 1.5 mg/l BA with 0.5 mg/l IAA combinations. Quantitative expression of eight epigenetic regulatory genes; Chromomethylase 3 (CMT3), Domains Rearranged Methyltransferase2 (DRM2), Fertilization Independent Endosperm (FIE2) and Chromodomain Protein Like Heterochromatin Protein1 (LHP1), Pickle (PKL), Proliferating Cell Nuclear Antigen (PCNA), Shoot Meristemless (STM) and DICER-LIKE1 (DCL1) have been estimated in the peach regenerated shoots. We found that the auxins and cytokinins in different combinations especially BA effect on the expression of some epigenetic genes in relation to the mature embryo.

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Section: Dna Methylationmentioning

confidence: 87%

Section: Chromatin Modificationsmentioning

confidence: 99%

Section: Dna Methylationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

Ismail

Ali

Nagaty

et al. 2020

Journal of Agricultural Chemistry and Biotechnology

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“…With this method, even recalcitrant cultivars of various crops can be successfully transformed (reviewed in Altpeter et al, 2016). Several sexual and apomictic Boecher a species have been investigated for their potential to be transformed by Agrobacterium , and it was reported to be possible to regenerate shoots from hypocotyl-derived calli of sexual B. stricta and apomictic B. gunnisoniana and B. holboellii (Taşkin et al, 2003, 2015). Somatic embryos derived from immature cotyledons of the apomict B. divaricarpa (Taşkin et al, 2009b) and, as a proof-of-concept, stable transformants of B. gunnisoniana were also generated (Taşkin et al, 2003).…”

Section: Molecular Tools For the Genus Boechera: Transformation Lasementioning

confidence: 99%

The Boechera Genus as a Resource for Apomixis Research

et al. 2019

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The genera Boechera (A. Löve et D. Löve) and Arabidopsis , the latter containing the model plant Arabidopsis thaliana , belong to the same clade within the Brassicaceae family. Boechera is the only among the more than 370 genera in the Brassicaceae where apomixis is well documented. Apomixis refers to the asexual reproduction through seed, and a better understanding of the underlying mechanisms has great potential for applications in agriculture. The Boechera genus currently includes 110 species (of which 38 are reported to be triploid and thus apomictic), which are distributed mostly in the North America. The apomictic lineages of Boechera occur at both the diploid and triploid level and show signs of a hybridogenic origin, resulting in a modification of their chromosome structure, as reflected by alloploidy, aneuploidy, substitutions of homeologous chromosomes, and the presence of aberrant chromosomes. In this review, we discuss the advantages of the Boechera genus to study apomixis, consider its modes of reproduction as well as the inheritance and possible mechanisms controlling apomixis. We also consider population genetic aspects and a possible role of hybridization at the origin of apomixis in Boechera. The molecular tools available to study Boechera , such as transformation techniques, laser capture microdissection, analysis of transcriptomes etc. are also discussed. We survey available genome assemblies of Boechera spp. and point out the challenges to assemble the highly heterozygous genomes of apomictic species. Due to these challenges, we argue for the application of an alternative reference-free method for the comparative analysis of such genomes, provide an overview of genomic sequencing data in the genus Boechera suitable for such analysis, and provide examples of its application.

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DNA Methylation and Methylation Polymorphism in Genetically Stable In vitro Regenerates of Jatropha curcas L. Using Methylation-Sensitive AFLP Markers

Rathore

Jha

2015

Appl Biochem Biotechnol

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The present investigation aimed to evaluate the degree and pattern of DNA methylation using methylation-sensitive AFLP (MS-AFLP) markers in genetically stable in vitro regenerates of Jatropha curcas L.. The genetically stable in vitro regenerates were raised through direct organogenesis via enhanced axillary shoot bud proliferation (Protocol-1) and in vitro-derived leaf regeneration (Protocol-2). Ten selective combinations of MS-AFLP primers produced 462 and 477 MS-AFLP bands in Protocol-1 (P-1) and Protocol-2 (P-2) regenerates, respectively. In P-1 regenerates, 15.8-31.17 % DNA was found methylated with an average of 25.24 %. In P-2 regenerates, 15.93-32.7 % DNA was found methylated with an average of 24.11 %. Using MS-AFLP in P-1 and P-2 regenerates, 11.52-25.53 % and 13.33-25.47 % polymorphism in methylated DNA was reported, respectively. Compared to the mother plant, P-1 regenerates showed hyper-methylation while P-2 showed hypo-methylation. The results clearly indicated alternation in degree and pattern of DNA methylation; hence, epigenetic instability in the genetically stable in vitro regenerates of J. curcas, developed so far using two different regeneration systems and explants of two different origins. The homologous nucleotide fragments in genomes of P-1 and P-2 regenerates showing methylation re-patterning might be involved in immediate adaptive responses and developmental processes through differential regulation of transcriptome under in vitro conditions.

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Determination of the expression levels of DNA methyltransferase genes during a highly efficient regeneration system via shoot organogenesis in the diploid apomict Boechera divaricarpa

Cited by 6 publications

References 67 publications

Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

Expression of some Epigenetic-Related Genes in Regenerated Shoots of Prunus persica

The Boechera Genus as a Resource for Apomixis Research

DNA Methylation and Methylation Polymorphism in Genetically Stable In vitro Regenerates of Jatropha curcas L. Using Methylation-Sensitive AFLP Markers

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