Determination of the enzyme destruction rational mode of biomass autolysate of lactic acid bacteria

Kapustian, А.; Cherno, Natalia; Станкевич, Георгій Миколайович; Kolomiiets, I. A.; Matsjuk, Oksana; Musiy, L. Y.; Slyvka, I. M.

doi:10.15587/1729-4061.2018.120877

Cited by 2 publications

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“…В літературі є окремі розрізнені та неповні й суперечливі дані стосовно способів нівелювання патогенетичних механізмів порушення функціонального стану організму качок у критичні періоди постнатального онтогенезу за дії стресу, які обмежені певними віковими групами (Olubodun et al, 2015). Актуальним залишається вивчення ефективності включення в раціон качок біологічно сумісних, нешкідливих кормових добавок, що сприяє нормалізації перебігу адаптаційнокомпенсаторних реакцій в їхньому організмі у критичні періоди постнатального онтогенезу і може слугувати антистресовою профілактикою в умовах промислового вирощування цього виду птиці (Pavlova, 2015;Kapustian et al, 2018). Мета роботи -з'ясувати реалізацію адаптаційного синдрому у системі показників неспецифічної резистентності організму качок при включенні в раціон біологічно активної кормової добавки (БАКД) «Праймікс Біонорм-К» та добавки «Біовір».…”

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Adaptation of the status of non-specific resistance of the ducks organism in stress conditions inclusion in the ration of probiotical additives

2018

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The article deals with the question of adaptation of the ducks organism during industrial cultivation for action of stress. The complex of successive stage reactions, caused by the functioning of stress-implementing systems, arise in the body of the poultry in response to the influence of destabilizing factors and aimed at neutralizing these factors, preservation of homeostasis. Increasing the influence of technological stress factors leads to a decrease in the immune status of the duck organism. The study of the effectiveness of incorporating into the diet ducks harmless feed additives, which contributes to the normalization of the course of adaptive-compensatory reactions in their body during the critical periods of postnatal ontogenesis and can serve as anti-stress prevention in conditions of industrial growth of this species of poultry remains relevant. The purpose of the work is to find out the implementation of an adaptation syndrome in the system of indices of non-specific resistance of the body of ducks when it is included in the diet of the biologically active feed supplement «Primix Bionorm K» and supplements «Biovir». The experiment was conducted on clinically healthy young ducks of the Pekin breed of egg production line. In the 270-day age, all three groups of ducks were modeled experimental technological stress in the form of prolonged transport. From 240 to 270 days of age, ducks additionally fed the additive «Primix Bionorm K» and was pouring with water an additive «Biovir». The material for research was blood, which was taken until morning feeding after slaughter on 240 days of life (to stress), 270 days of life (stage of anxiety), at 273 and 285 days (stages of resistance). Adaptation of the state of non-specific resistance of the duck of the control group to the control group under conditions of stress in the anxiety stage is characterized by an increase of 17.3% (Р < 0.05) of the value of the bactericidal activity of the blood serum, a decrease of 8.5% of the value of lysozyme activity of the blood serum, by 9.3% of phagocytic activity against the background of growth on 8.7% of the number of circulating immune complexes compared to 240-day-old poultry. At the stage of resistance there is a decrease in the amount of blood serum bactericidal activity by 17.3% (Р < 0.05), an increase in the activity of lysozyme in blood serum by 8.5%, and the number of immune complexes by 20.7% (Р < 0.05), with the stabilization of the studied parameters at the later stages of the development of a stress reaction. In these periods, a decrease in the body weight of control group ducks was set at 5.7 – 6.8%. Feeding of the additive «Primix Bionorm K» during the period of the implementation of the stress syndrome has a positive effect on the indices of the cellular and humoral level of nonspecific resistance in the form of an increase in the amount of bactericidal activity of the serum on 38.2% (P < 0.05), an increase in the activity of phagocytosis by 15.1% (P < 0.05), phagocytic index – by 36.1% (P < 0.05) with a decrease in the number of circulating immune complexes by 15.5%. The presentation of the additive «Biovir» causes an increase in the level of bactericidal and lysozyme activity of the blood serum on 47.3% (P < 0.01) and 18.7% (P < 0.05). The use of additives can reduce the negative effect of transport stress in the duck body, increase nutrient uptake, as indicated by an increase in the body weight of experimental birds in the average by 7.1–10.5% (P < 0.05) during the development of the adaptive syndrome.

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Adaptation of the status of non-specific resistance of the ducks organism in stress conditions inclusion in the ration of probiotical additives

2018

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Optimization of Parameters of Fermentolysis of Proteins in the Composition of Serum-Protein Concentrate

Ткаченко¹,

Lanzhenko²,

Скрипніченко³

et al. 2019

ХНТ

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The food industry is a strategic industry that works quite steadily even during periods of economic crises, providing food security to any state, and is a source of raw material for other industries with a high potential for development, for example, for the production of cosmetics. The modern cosmetics market is represented by various cosmetic products, often expensive, but not always made from natural ingredients. Therefore, the search for the newest ingredients for the production of natural cosmetics on the basis of domestic raw materials is an urgent task of the present. Ingredients from milk serum can be used for the production of natural cosmetics that in large quantities is obtained in milk processing enterprises and often remains unprocessed. Whey protein concentrations can be a source of short-chain peptides and free amino acids for the production of various cosmetic products. The process of fermentolysis of serum proteins in nanofiltration concentrate KSB-65 with the content of dry matter of 20% using neutral peptidase C from the domestic producer at a temperature of 40 ºС with the duration of the process varying from 1 to 5 hours, the content of peptidase – from 0,5 to 2,0 U/g. It is established that the optimal parameters of the fermentolysis of serum proteins in KSB-65 are as follows: temperature 40 º C, neutral peptidase C content – 0.78 U/g, duration of fermentolysis – 3.17 hours. With optimal parameters of the fermentolysis process, the hydrolyzate of the nanofiltration concentrate KSB-65 contains the maximum amount of short chain peptides (57.03 mg/cm3) and a high concentration of free amino acids (54.66 μg/cm3). Recommendations for the further use of serum protein hydrolyzate obtained using the recommended optimal parameters of the enzyme production process from the nanofiltration concentrate KSB-65, in the manufacture of cosmetic products, including with anti-age effect, and hydrolyzates of proteins enriched with probiotic cultures of lactam bifidobacteria or their lysates.

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Determination of the enzyme destruction rational mode of biomass autolysate of lactic acid bacteria

Cited by 2 publications

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Adaptation of the status of non-specific resistance of the ducks organism in stress conditions inclusion in the ration of probiotical additives

Adaptation of the status of non-specific resistance of the ducks organism in stress conditions inclusion in the ration of probiotical additives

Optimization of Parameters of Fermentolysis of Proteins in the Composition of Serum-Protein Concentrate

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