Determination of the Effects of Z. officinale and Propolis Extracts on Intestinal Microbiology and Histological Characteristics in Broilers

Tekeli, Ahmet; Kutlu, Hasan Rüştü; Çelik, Ladine; Doran, Figen

doi:10.3923/ijps.2010.898.906

Cited by 26 publications

(21 citation statements)

References 8 publications

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“…Thus, the increase in crypt depth in chickens fed with propolis indicates that more energy could be required for cell renewal in the ileum, which could be related to the lack of effect of crude propolis inclusion on chicken performance. These results are unexpected considering the higher mucosa development seen in chickens fed with bee pollen (Wang et al 2007) and the positive effects on the intestinal morphology of chickens fed propolis extract, shown by Tekeli et al (2010) and Eyng et al (2013).…”

Section: Resultscontrasting

confidence: 42%

“…However, it is possible that changes in large intestine weight presented by quadratic response can be attributed to the modulatory effect of propolis on the microbiota, as shown by Tekeli et al (2010) and Kacˇa´niova´et al (2012), which in turn could decrease pathogenic bacteria and consequently the inflammatory process caused by the pathogens and toxins produced by these bacteria, reducing the thickness and weight of large intestine. However, this effect seems to be dose-dependent, as showed by Ç etin et al (2011), given that the lowest intestine weight was estimated at 278 ppm of crude propolis, which could indicate a lower adherence of pathogenic bacteria, with increased intestinal weight at levels above or below this level.…”

Section: Resultsmentioning

confidence: 99%

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Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

et al. 2014

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. 2014. Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis. Can. J. Anim. Sci. 94: 105Á114. Propolis is a resin produced by bees that has been investigated for its potential to increase the performance of broiler chickens. Therefore, this study aimed to evaluate the inclusion of crude propolis in the diets of broiler chickens from 1 to 21 d of age. A total of 1020 male chicks were distributed in a completely randomized experimental design into six treatments (0, 100, 200, 300, 400 and 500 ppm of crude propolis), with five replications, and 34 birds per experimental unit. The experimental diets were provided from 1 to 21 d of age and subsequently (from 22 to 42 d of age) the birds were fed a nutritionally complete ration based on corn and soybean meal. All supplemented groups showed similar growth performance in all growth phases (P!0.05); however, there was a linear increase in carcass yield at 42 d of age according to increase in crude propolis inclusion from 1 to 21 d of age (PB0.05). At 7 d of age, relative weight of the gizzard increased linearly (PB0.05) with increasing propolis, and at 21 d of age, relative weight of the large intestine showed a quadratic (P B0.05) response, with the lowest value predicted to occur at 278 ppm of crude propolis. The ileum crypt depth at 21 d of age was affected by propolis, with a quadratic response (P B0.05) and highest value estimated at 323 ppm. At 21 d of age, sucrase activity in the jejunum increased linearly with increasing propolis and maltase activity in the ileum showed a quadratic (PB0.05) response, which predicted highest activity at 292 ppm. Amylase activity was lower in animals supplemented with 100 and 400 ppm of propolis at 21 d of age (PB0.05) compared with the control group. Thus, the crude propolis supplementation is not recommended in broiler diets from 1 to 21 d old given that it does not influence the performance, despite small changes in intestinal morphophysiology at 21 d of age.

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Section: Resultscontrasting

confidence: 42%

Section: Resultsmentioning

confidence: 99%

Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

et al. 2014

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“…colonization in gut. Also the current results are similar to those of Abouda et al, (2011);Kačániová et al, (2013) and Tekeli et al, (2010). Abouda et al, (2011) reported that bee pollen inhibited the proliferation of some microorganisms.…”

Section: Resultssupporting

confidence: 81%

“…Attia et al, (2011) reported that inclusion 200 mg pollen increased New Zealand White Bucks growth performance. Also, abouda et al, (2011);Kačániová et al, (2013) and Tekeli et al, (2010) reported that pollen have an antimicrobial effects on pathogenic microorganisms in the gut of broilers chicks. In literature, there are studies about pollen on performance, gut histology and gut microbiota, but there is no study about in ovo injection of pollen extract on performance, gut histology and gut microbiota of broiler chicks.…”

Section: Introductionmentioning

confidence: 99%

The effects of in ovo pollen extract injection on growth parameters, ileal histomorphology and caecal microflora in fasted broiler chicks

Coşkun

Korkmaz

Altop

et al. 2017

IJAR

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This study was carried out to determine the effects of in ovo pollen extract injection on growth parameters, ileal histomorphology, and caecal microflora of fasted broiler chicks. In this experiment, 2×2 factorial experimental design was used. One d old, 120 healthy broiler chicks were allocated to 4 treatment groups and 6 replicates (5 mixed sex chicks allocated each replicates). Treatment groups were: A) Pollen extract injection and 24 h fasting (P24); B) Pollen extract injection and 48 h fasting (P48), C) Control, no injection 24 h fasting (C24) and D) Control, no injection 48 h fasting (C48). The experiment lasted 21 days. Live weight, feed consumption, feed conversion ratio were recorded weekly. Ileal histomorphology, caecal microbiota, organ weight were recorded at 21th days of experiment. In ovo pollen extract injection did not affect hatchability rate. At the end of 21 days, in ovo pollen extract injection did not affect feed intake, live weight gain, feed conversion ratio, inner organ development and ileal villi width irrespective to fasting 24 h and 48 h. In ovo pollen extract injection increased ileal villi length, caecal lactic acid bacteria and Saccharomyces Cerevisiae count, decreased caecal Enterobactericaea count. In conclusion, in ovo pollen extract injection can be applied for broiler eggs to improve weight gain, better digestion and gut health.

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“…Nevertheless, when examining treatments C, E1, E2 and E3, it was shown that increasing dietary PFA inclusion level resulted in increasing Vh/Cd ratio in the duodenum of 14-day-old broilers and ileal Vh in 42-day-old broilers. An increase in jejunal Vh has also been reported in broilers fed Zingiber officinale, propolis or a Z. officinale 1 propolis combination (Tekeli et al, 2010). Generally, Vh and Vh/Cd ratio represents an indicator of the digestive capacity of the small intestine and higher values are concomitant with improved digestion and absorption (Montagne et al, 2003).…”

mentioning

confidence: 88%

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

Tsirtsikos

Fegeros

Kominakis

et al. 2012

Animal

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The effect of a dietary phytogenic feed additive (PFA) inclusion level in mucin monosaccharide composition, mucosal morphometry and mucus histochemistry along the broiler intestinal tract was studied. Cobb male broilers (n 5 525) were allocated into five experimental treatments that, depending on the type of addition in the basal diet (BD), were labeled as follows: C (BD based on maize-soybean meal with no other additions), E1 (80 mg PFA/kg BD), E2 (125 mg PFA/kg BD), E3 (250 mg PFA/kg of BD) and A (2.5 mg avilamycin/kg BD). Samples from duodenum, ileum and cecum of 14-and 42-day-old broilers were collected and analyzed. In 14-day-old broilers, treatments E2 and E3 had higher (P , 0.01) duodenal mannose than treatments C, E1 and A. Ileal mannose was lower (P , 0.05) in treatment C compared with PFA treatments, and ileal galactose (Gal) was higher (P , 0.01) in treatments E2 and E3 compared with C and A. Polynomial contrast analysis with respect to PFA inclusion level showed that in 14-day-old broilers there was a linear increase (P 5 0.001) in duodenal mannose and a quadratic effect (P 5 0.038) in duodenal N-acetyl-galactosamine with increasing PFA level. Ileal Gal and mannose increased linearly (P 5 0.002 and P 5 0.012, respectively) with PFA inclusion level. There were no significant differences between treatments in mucin monosaccharide molar ratios of 42-day-old broilers. However, increasing PFA inclusion level resulted in a linear decrease of ileal fucose (P 5 0.021) and cecal N-acetylgalactosamine (P 5 0.036). Experimental treatments did not differ (P . 0.05) regarding duodenal villus height (Vh), crypt depth (Cd) and Vh/Cd ratio, irrespective of broiler age and the intestinal segment examined. However, increasing dietary PFA inclusion level showed a pattern of linear increase of duodenal Vh/Cd ratio in 14-day-old broilers and ileal Vh in 42-day-old broilers (P 5 0.039 and P 5 0.039, respectively). Alcian Blue-Periodic AcidSchiff (pH 2.5) staining of neutral and acidic mucins showed that the staining intensity of mucus layer in villi was fragment (i.e. tip, midsection and base) dependent, whereas in crypts it was dependent both on intestinal segment (i.e. duodenum, ileum and cecum) and fragment. Finally, mucus layer thickness did not differ (P . 0.05) between treatments, yet a pattern of linear increase (P , 0.05) with PFA inclusion level was observed in the duodenum of 42-day-old broilers. In conclusion, the dietary inclusion level of PFA modulated broiler intestinal mucin composition and morphology. Further studies are required to elucidate the physiological implications of such changes in host-microflora interactions.Keywords: phytogenic feed additive, essential oils, broiler, mucin, intestinal morphology ImplicationsThis study has shown that the inclusion level of a phytogenic feed additive in broiler diets has resulted in changes in the gut mucin monosaccharide composition and the intestinal mucosal architecture morphology. Given the fact that animal performance and disease prevention are prerequisite...

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Determination of the Effects of Z. officinale and Propolis Extracts on Intestinal Microbiology and Histological Characteristics in Broilers

Cited by 26 publications

References 8 publications

Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

Intestinal morphology and activity of digestive enzymes in broilers fed crude propolis

The effects of in ovo pollen extract injection on growth parameters, ileal histomorphology and caecal microflora in fasted broiler chicks

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

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