Determination of the distribution and multiplication sites of Flavescence Dorée mycoplasma-like organisms in the host plant Vicia faba by ELISA and immunocytochemistry

Lherminier, Jeannine; Courtois, María F; Caudwell, A.

doi:10.1016/s0885-5765(05)80071-2

Cited by 32 publications

(15 citation statements)

References 26 publications

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“…Young phytoplasma elementary bodies, similar to those reported in the literature (Hiruki 1988;D'Agostino 1991;Lherminier at al. 1994), were more common towards the apex of the root, but this morphotype was less frequent in AMP plants.…”

Section: Discussionsupporting

confidence: 88%

Mycorrhiza-induced differential response to a yellows disease in tomato

et al. 2002

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The protective effects induced by arbuscular mycorrhizal (AM) fungi against a phytoplasma of the Stolbur group have been investigated in tomato by morphometry and flow cytometry. Symptoms induced by the phytoplasma were less severe when the plants also harboured AM fungi. Morphological parameters such as shoot and root fresh weight, shoot height, internode length, leaf number and adventitious root diameter were closer to those of healthy plants when arbuscular mycorrhiza were present. Reduced nuclear senescence was observed in AM plants infected with phytoplasmas; the percentages of nuclear populations with different ploidy levels were intermediate between AM and phytoplasma-infected plants. The mechanisms underlying these interactions are discussed and a direct action of the AM fungus is hypothesized.

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Section: Discussionsupporting

confidence: 88%

Mycorrhiza-induced differential response to a yellows disease in tomato

et al. 2002

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“…Considering the developmental feeding stage of the vector, it is likely that young nymphs spend less time than older instars in feeding on diseased grapevines. Third, the concentration of FDP in grapevine tissues may be very low and phytoplasma cells localized in sparsely infected sieve tubes in grapevine tissues during the first part of the season, with the pathogen progressively invading the phloem tissues and increasing its titre as the season progresses, as described for other host plants or phytoplasmas (Kuske & Kirkpatrick, 1992; Lherminier et al., 1994; Wei et al., 2004). It is thus likely that the increasing proportion over time of FDP‐infected leafhoppers in a population reflects the seasonal increase of phytoplasma multiplication and spread within grapevines.…”

Section: Discussionmentioning

confidence: 99%

Patterns of phytoplasma‐infected and infective Scaphoideus titanus leafhoppers in vineyards with high incidence of Flavescence dorée

Bressan

Larrue²,

Padieu³

2006

Entomologia Exp Applicata

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The incidence and transmissibility of Flavescence dorée phytoplasma (FDP) in populations of the vector Scaphoideus titanus Ball (Homoptera: Cicadellidae) were investigated by periodically collecting nymphs and adults of the leafhopper species in four vineyards with high incidence of Flavescence dorée (FD)‐diseased grapevines. Insects were tested individually for FDP with an ELISA procedure, after transmission assays to broadbean seedlings and further transmission to grapevine cuttings. No transmission occurred when early or middle instar nymphs were used to inoculate broadbeans, although a limited number of fifth‐instar nymphs and young adults transmitted the pathogen to broadbean seedlings. However, the same batches of insects transmitted FDP more efficiently to grapevine cuttings during prolonged inoculation periods, confirming the existence of a latent period before infected insects become infective. The proportions of ELISA‐positive individuals in the three categories of insects used for transmission assays reflected the rate of FDP transmission to grapevine cuttings. According to the data obtained by ELISA and from field sampling of first‐instar nymphs, we adapted the proportions of nymph hatching, of infected leafhoppers, and of infective leafhoppers (assuming a conservative latent period in the vector of 30 days) to logistic models as a function of degree‐days. We then discussed the possible use of the model developed for improving vector control decisions in FD‐infected vineyards.

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“…Antibodies raised against several phytoplasmas were produced previously, including aster yellows (Barbara et al 1998;Errampalli and Fletcher 1993;Jiang et al 1988;Lin and Chen 1985), Western X disease (Blomquist et al 2001), clover phyllody , apple proliferation (Berg et al 1999), sweet potato witches' broom (Shen and Lin 1993;Shen and Lin 1994;Yu et al 1998), grapevine flavescence dorée (Lherminier et al 1994;Seddas 1993), stolbur (Fos et al 1992), faba bean phyllody (Saeed et al 1992), rice yellow dwarf (Chang et al 1995;Onuki et al 1992), China tree (Melia azedarach L.) decline (Gomez et al 1996), and primula yellows (Clark et al 1989;Milne et al 1995). These antibodies have provided important tools for the analysis of phytoplasmas such as detection, localization, and purification of the phytoplasma or the target antigens.…”

Section: Discussionmentioning

confidence: 99%

Cloning and Expression Analysis of Phytoplasma Protein Translocation Genes

Kakizawa¹,

Oshima²,

Kuboyama³

et al. 2001

MPMI

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Genes encoding SecA and SecY proteins, essential components of the Sec protein translocation system, were cloned from onion yellows phytoplasma, an unculturable plant pathogenic bacterium. The secA gene consists of 2,505 nucleotides encoding an 835 amino acid protein (95.7 kDa) and shows the highest similarity with SecA of Bacillus subtilis. Anti-SecA rabbit antibody was prepared from a purified partial SecA protein, with a histidine tag expressed in Escherichia coli. Western blot analysis confirmed that SecA protein (approximately 96 kDa) is produced in phytoplasma-infected plants. Immunohistochemical thin sections observed by optical microscopy showed that SecA is characteristically present in plant phloem tissues infected with phytoplasma. The secY gene consists of 1,239 nucleotides encoding a 413 amino acid protein (45.9 kDa) and shows the highest similarity with SecY of B. subtilis. These results suggest the presence of a functional Sec system in phytoplasmas. Because phytoplasmas are endocellular bacteria lacking cell walls, this system might secrete bacterial proteins directly into the host cytoplasm. This study is what we believe to be the first report of the sequence and expression analysis of phytoplasma genes encoding membrane proteins with a predicted function.

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Determination of the distribution and multiplication sites of Flavescence Dorée mycoplasma-like organisms in the host plant Vicia faba by ELISA and immunocytochemistry

Cited by 32 publications

References 26 publications

Mycorrhiza-induced differential response to a yellows disease in tomato

Mycorrhiza-induced differential response to a yellows disease in tomato

Patterns of phytoplasma‐infected and infective Scaphoideus titanus leafhoppers in vineyards with high incidence of Flavescence dorée

Cloning and Expression Analysis of Phytoplasma Protein Translocation Genes

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