Determination of the chemical composition and antimicrobial activity of Frankenia hirsuta

Abstract

<p class="Abstract">Frankenia hirsuta is widely located in Turkey, but the antimicrobial potential and biochemical composition analysis of it weren’t determined yet. By using the disk diffusion method, the susceptibility of 17 bacteria and 1 fungi were analyzed, which included Bacillus, Candida, Enterobacter, Enterococcus, Esche-richia, Klebsiella, Listeria, Pseudomonas, Salmonella and Staphylococcus genera. 0.8, 1.5 and 3.1 mg of samples were prepared by using absolute ethanol. The bioactive composition of the plant extract was determined by gas chromato-graphy-mass spectroscopy, and National Institute of Standards and Techno-logy library was used for the mass spectra analysis. The results showed that F. hirsuta had antimicrobial activity against all of the studied micro-organisms except E. aerogenes and E. coli. Several active metabolites were identified, but some composition of this sample didn’t match with the library. These results are the first report for the antimicrobial potential and biochemical composition of F. hirsuta.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract">3 min 36 sec: <a href="https://www.youtube.com/v/YeKtYxtaa6c">Full Screen</a> <a href="https://www.youtube.com/watch?v=YeKtYxtaa6c">Alternate</a></p>

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“…Antimicrobial Assay: Antimicrobialanalysis was followed using a standard agar well diffusion method to study the antimicrobial activity of compounds 31,33 . Each bacterial isolate was suspended in Brain Heart Infusion (BHI) broth and diluted to approximately 10 5 colony-forming units (CFU) per mL.…”