Determination of the binding affinities of plasmid DNA using fluorescent intercalators possessing an acridine skeleton

Cytotoxic activity of acridin-3,6-diyl dithiourea hydrochlorides in human leukemia line HL-60 and resistant subline HL-60/ADR

“…2, the DNA melting experiment revealed that T m of the calf thymus DNA was 73.0 • C and in the presence of AcrDTU increased to 77.0-80.0 • C (Table 2), thus emphatically confirming heightened helix stability as the result of intercalation of proflavine derivatives into DNA. These findings are consistent with our previous results [14,17].…”

“…Blasiak et al [13] confirmed a possible modulation of amsacrine cytotoxicity by some free radical scavengers. Acridine analogues have also been evaluated for their strong DNA-binding activity [14] and cytotoxic potency toward sensitive and multidrugresistant tumor cell lines [15,16]. The planar acridine ring system stacks well with the base pairs in the intercalation complex.…”

DNA binding properties and evaluation of cytotoxic activity of 9,10-bis-N-substituted (aminomethyl)anthracenes

“…On the basis of the structure of compounds 3a-3d we suppose that the high- est cytotoxic and binding activities of 3c might be assigned to the presence of basic nitrogen atoms at the end of both piperazine moieties. Consequently, the effect of substituents within our series, parallel to those given in the paper of Sabolová et al [2], seems to be more complex.…”

“…A search for new, low-molecular weight ligands which can specifically bind to DNA has been conducted for many years in the hope that new therapeutics could selectively modulate aberrant gene expression [1]. In our previous works, we determined the binding affinity and binding constants of N-(acridin-9-ylthiocarbamoyl)amino acids and proflavine-dithiazolidinones with plasmid DNA [2,3]. In an effort to synthesize new types of DNA intercalators, we have now turned our attention to anthracene derivatives [4][5][6].…”

Synthesis of Novel Eu(III) Luminescent Probe Based on 9- Acridinecarboxylic Acid Skelton for Sensing of ds-DNA

“…The interaction of fluorescent metal complexes containing multidentate aromatic ligands with DNA has gained much attention. This is due to their possible application as new therapeutic agents and their attractive emission properties such as long lifetime; large Stokes' shift, and line like emission, which make them potential probes of DNA structure and its conformation [7,18]. Acridines have been a topic of interest for a long time owing to their biological activities, numerous applications and the ability to intercalate tightly to DNA helical structure [3].…”