Determination of the Antioxidants’ Ability to Scavenge Free Radicals Using Biosensors

In vitro anti oxidant activity total phenolic content of Dillenia indica Garcinia penducalata, commonly used fruits in Assamese cuisine

“…[2][3][4][5][6] Oxi dative stress is initiated by reactive oxygen species (ROS) such as hydrogen peroxide and superoxide anions, that react with the living tissues and cause harmful effects such as lipid peroxidation, DNA fragmentation and cross linking of pro teins and enzymes in them thereby causing diseases. [7] Human diet can be a major source of antioxidants. Poly phenols which are organic compounds present in regular human diet have an antioxidant property.…”

Antioxidants: Terminology, Methods, and Future Considerations

“…Antioxidant properties are proposed to be defined as “antioxidant capacity” [6,19] and “antioxidant activity”, “antioxidant power” [20], “antioxidant ability” [5]. The first one is interpreted as a “measure of the moles of given free radical scavengers by a test solution”.…”

“…The latter is very important both for clinical studies and for the selection of therapeutic agents, their quality control and optimization of the therapy and diet, and for assessing the quality of food, nutrients, cosmetics, and pharmaceuticals. The problems of monitoring are determined by the complexity of the biological matrix and rapid change of its composition after sampling; variety of compounds of different chemical nature, possessing oxidative and antioxidant properties; a short period of life of radical compounds that play a major role in the processes of life support; the absence of a single term and comparable expression units of concentration and antioxidant properties of a compound or a complex of compounds; and use for quantifying oxidative stress of bioorganic molecules with various properties that do not always correctly simulate processes in a living organism [1,2,3,4,5,6]. Thus, the use of stable radicals, including those generated during the analysis, makes the interpretation of the results concerning oxidative stress insufficiently correct, since the nature and chemical transformations of these and natural radicals vary greatly.…”

Electrochemical Methods for Total Antioxidant Capacity and its Main Contributors Determination: A review

“…To solve this drawback, another type of bioanalytical tool was developed, represented by the cytochrome-based biosensors, based on redox reactions involving this oxidase [128,129].…”

“…Catalase addition helps avoid interference from H 2 O 2 , that may occur from O 2 ·-disproportion. In this manner, the presence of antioxidants diminishes the radical amount and, proportionately, the measured intensity value resulting in viable quantitation of the antioxidant activity in different analysed matrixes [127][128][129].…”