Determination of the Antioxidant Activity of Samples of Tea and Commercial Sources of Vitamin C, Using an Enzymatic Biosensor

Ribeiro, Danilo Braga; Silva, Gabriela Santos; Santos, Djanira Rubim Dos; Costa, Andressa Rose Castro; Ribeiro, Eliane Braga; Badea, Mihaela; Nunes, Gilvanda Silva

doi:10.3390/antiox10020324

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…Furthermore, the assessment of antioxidant capacity was conducted by employing identical enzymes through the utilization of polymeric PVA-AWE films that were immobilized on the carbon paste electrode surface. The chamomile sample exhibited the highest antioxidant capacity, which was approximately 70% [94]. The present study focuses on the utilization of an ascorbate oxidase-based sensor-biosensor system (SB) for the purpose of detecting ascorbic acid as well as several types of orange juice, specifically Hamlin, Sanguinello, and Moro.…”

Section: Oxidase-based Biosensormentioning

confidence: 99%

Antioxidant Determining Using Electrochemical Method

Sukma,

Iswantini,

Nurhidayat

et al. 2023

Chemistry

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Antioxidants are very beneficial for health as they protect the body from the effects of free radicals on various degenerative diseases caused by food contamination, air pollution, sunlight, etc. In general, methods for measuring the capacity of antioxidants generally use accurate methods such as spectrophotometry and chromatography. Still, this takes time, accurate sample preparation, and must be performed in a laboratory with particular expertise. Therefore, a new, more practical method needs to be developed for determining antioxidants, namely the electrochemical method. The electrochemical method is a promising method to develop because it comes with several advantages, including high sensitivity and fast response. The electrochemical method discussed in this article reviews sensors, biosensors, and nanosensors. This paper comprehensively analyzes contemporary developments in electrochemical biosensor techniques and antioxidant evaluation methodologies. The discussion centers on utilizing multiple biosensors. Electrochemical biosensors have been determined to be prevalent in analyzing food quality, assessing active factor functionality, and screening practical components. The present study outlines the difficulties linked with electrochemical bio-sensor technology and provides insights into the potential avenues for future research in this domain.

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Section: Oxidase-based Biosensormentioning

confidence: 99%

Antioxidant Determining Using Electrochemical Method

Sukma,

Iswantini,

Nurhidayat

et al. 2023

Chemistry

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“…neutralizing the free radicals or ROS before they can attack the cells. A wide variety of antioxidants from both natural and synthetic origins have been discovered for use in the treatment of various human diseases 11 .…”

Section: Introduction: Cichorium Intybus Lmentioning

confidence: 99%

Untitled

2021

IJPSR

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Common chicory (Cichorium intybus L.) plant belonging to sunflower family primarily grown for its root which is used as a coffee substitute but is also widely used in folk medicine to treat various ailments ranging from wounds to diabetes. The plant is a good tonic cooling agent and is useful in headache, throat inflammation, immune stimulation, mutagenic, probiotic, hepatoprotective, antibacterial activity etc., and these actions are attributed to a wide range of phytoconstituents present in this plant. In the existing study, the total flavonoid and phenol content of methanolic extract of selected parts (leaves, root, and seeds) of Cichorium intybus L.was quantitatively estimated using aluminium chloride colorimetric method (as quercetin equivalent) and Folin-Ciocalteu method (as caffeic acid equivalent) respectively. The total phenolic contents varied from 1.94± 0.895 to 1.66± 0.506 mg/gdw. Total flavonoid contents were between 1.60± 1.69 to 1.37 ± 0.796 mg/gdw.

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“…Conventional approaches for detecting antioxidants mainly involve the use of biorecognition elements − or multiple oxidant reagents. − Electrochromic (EC) sensors, on the other hand, require no additional chemicals and therefore no following frustrating washing steps, which directly interact with antioxidants and have the ability to color change without relying on chromogenic substrates.…”

mentioning

confidence: 99%

Electrochromic Sensor Augmented with Machine Learning for Enzyme-Free Analysis of Antioxidants

Ranjbar,

Salavati,

Ashari Astani

et al. 2023

ACS Sens.

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Our study presents an electrochromic sensor that operates without the need for enzymes or multiple oxidant reagents. This sensor is augmented with machine learning algorithms, enabling the identification, classification, and prediction of six different antioxidants with high accuracy. We utilized polyaniline (PANI), Prussian blue (PB), and copper−Prussian blue analogues (Cu-PBA) at their respective oxidation states as electrochromic materials (ECMs). By designing three readout channels with these materials, we were able to achieve visual detection of antioxidants without relying on traditional "lock and key" specific interactions. Our sensing approach is based on the direct electrochemical reactions between oxidized electrochromic materials (ECMs ox ) as electron acceptors and various antioxidants, which act as electron donors. This interaction generates unique fingerprint patterns by switching the ECMs ox to reduced electrochromic materials (ECMs red ), causing their colors to change. Through the application of density functional theory (DFT), we demonstrated the molecular-level basis for the distinct multicolor patterns. Additionally, machine learning algorithms were employed to correlate the optical patterns with RGB data, enabling complex data analysis and the prediction of unknown samples. To demonstrate the practical applications of our design, we successfully used the EC sensor to diagnose antioxidants in serum samples, indicating its potential for the on-site monitoring of antioxidant-related diseases. This advancement holds promise for various applications, including the real-time monitoring of antioxidant levels in biological samples, the early diagnosis of antioxidant-related diseases, and personalized medicine. Furthermore, the success of our electrochromic sensor design highlights the potential for exploring similar strategies in the development of sensors for diverse analytes, showcasing the versatility and adaptability of this approach.

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Determination of the Antioxidant Activity of Samples of Tea and Commercial Sources of Vitamin C, Using an Enzymatic Biosensor

Cited by 12 publications

References 39 publications

Antioxidant Determining Using Electrochemical Method

Antioxidant Determining Using Electrochemical Method

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Electrochromic Sensor Augmented with Machine Learning for Enzyme-Free Analysis of Antioxidants

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