Determination of the Antimicrobial and Antioxidative Properties and Total Phenolics of Two “Endemic” Lamiaceae Species from Turkey: Ballota rotundifolia L. and Teucrium chamaedrys C. Koch

Gürsoy, Nevcihan; Tepe, Bektaş

doi:10.1007/s11130-009-0115-2

Cited by 31 publications

(18 citation statements)

References 28 publications

(29 reference statements)

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“…Teucrium chamedrys and T. montanum are very rich in phenolic compounds with very strong biological activity and antioxidative effects (Č anadanović- Brunet et al 2006;Ozgen et al 2006;Gursoy and Tepe 2009;Stanković et al 2010;Stanković et al 2011b). Stankovic et al (2011a) reported that extracts from different Teucrium species caused cytotoxicity and induced apoptosis in human HCT-116 cells (human colon cancer cell line), hence they could be considered suitable natural anticancer products.…”

Section: Discussionmentioning

confidence: 99%

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

et al. 2013

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Since Teucrium chamaedrys and Teucrium montanum are the most popular plants used in the treatment of many diseases, we evaluated genotoxic potential of their methanolic extracts on cultured human peripheral blood lymphocytes (PBLs) using cytokinesis-block micronucleus (MN) assay. Cultures were treated with four concentrations of both plants (125, 250, 500 and 1,000 lg/ml), both separately and in combination with mitomycin C (MMC). The results revealed that extract of T. chamaedrys administered at the tested concentrations did not significantly affect the mean MN frequency in comparison to untreated cells. Methanolic extract of T. montanum increased the mean MN frequency in PBL at the tested concentrations, but significantly only at the concentration of 1,000 lg/ml. In all tested concentrations, the extract of T. chamaedrys significantly reduced the MMC-induced MN frequency, in a dose dependent manner (r = -0.687, p \ 0.01). The extract of T. montanum decreased the MMC-induced MN frequency at the tested concentrations, but statistically only at 125 lg/ ml. Both extracts administered alone did not significantly affect the nuclear division index (NDI) at the tested concentrations. In the combined treatments with MMC, the extract obtained from T. chamaedrys in the concentrations of 500 and 1,000 lg/ml significantly decreased NDI values in comparison to MMC-treated cells alone, while the extract of T. montanum significantly decreased NDI at all tested concentrations. Both extracts nonsignificantly decreased NDI at all tested concentrations in comparison to untreated cells. Our results suggest the important function of T. chamaedrys extract in cancer therapy, this methanolic extract may prevent genotoxic effects of chemotherapy in PBLs.

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Section: Discussionmentioning

confidence: 99%

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

et al. 2013

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“…Only some species of this genus have previously been evaluated for antimicrobial activities. Gursoy and Tepe [15] tested polar and non-polar subfractions of methanol extracts of Teucrium chamaedrys from Turkey. The polar sub-fraction exhibited weaker antimicrobial activity than the non-polar samples.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species

et al. 2012

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In vitro antimicrobial activity of 21 crude extracts obtained from seven taxa of the genus Teucrium (T. chamaedrys, T. montanum, T. arduini, T. polium, T. scordium subsp. scordium, T. scordium subsp. scordioides and T. botrys) was tested against bacterial and fungal species. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined using a microdilution analysis method. Total phenolic content and flavonoid concentrations were measured spectrophotometrically. Total phenols were determined using Folin-Ciocalteu reagent and their amounts ranged from 28.49 up to 159.84 mg CA/g of extract (chlorogenic acid equivalent). The amounts of flavonoids ranged from 38.17 up to 190.45 mg RU/g of extract (rutin equivalent).The plant extracts showed greater potential of antibacterial than antifungal activity. A relationship was found between total phenolics and biological activity. The highest level of total phenols was measured in the methanol extracts, which demonstrated higher antimicrobial activity than acetone and ethyl acetate extracts. Staphylococcus aureus ATCC 25923 appeared to be the most sensitive organism. Our results indicate that Teucrium spp extracts are rich sources of phenolic compounds and are promising candidates for further development as natural antimicrobial agents.

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“…2004). The DPPH assay and β‐carotene/linoleic acid test system were also reported as two complementary tests (Gursoy and Tepe 2009).…”

Section: Resultsmentioning

confidence: 99%

STUDIES ON ANTIOXIDANT PROPERTIES OF THE DIFFERENT SOLVENT EXTRACTS AND FATTY ACID COMPOSITION OFHYOSCYAMUS RETICULATUSL.

Güler

2011

Journal of Food Biochemistry

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Hyoscyamus reticulatus (Solanaceae) is distributed in the Mediterranean, East and Central Anatolia regions of Turkey. In the present study, antioxidant properties of the various extracts of H. reticulatus were evaluated by seven different chemical assays, namely total phenolics and flavonoids content, total antioxidant capacity, free radical (2,2‐diphenyl‐1‐picrylhydrazyl)‐scavenging assay, β‐carotene/linoleic acid test system, and ferric‐ and cupric ion‐reducing power. The strongest antioxidant properties were obtained by ethanolic extract. The order of the antioxidant properties of extracts were ethanolic > methanolic > acetone. Fatty acid composition of the aerial parts of H. reticulatus was analyzed by gas chromatography. The main fatty acids of the oil gave the proportions C 12:0 (lauric acid), C 10:0 (capric acid) and C 11:0 (undecanoic acid). Results of the present study demonstrated that the extracts and oil of H. reticulatus may be used as a source of natural antioxidant and lauric acid in the food and pharmaceutical industries. PRACTICAL APPLICATIONS Hyoscyamus species contain important tropane alkaloids, which have antispasmodic, anticholinergic, analgesic and sedative effects. In this study, antioxidant properties of the various extracts of Hyoscyamus reticulatus were investigated by seven different chemical assays. The results of the present study may be important as an indication of pharmacological and food processing utility of the plant. H. reticulatus can be considered as a source of both natural antioxidants and lauric acid in food industry and pharmacological applications.

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Determination of the Antimicrobial and Antioxidative Properties and Total Phenolics of Two “Endemic” Lamiaceae Species from Turkey: Ballota rotundifolia L. and Teucrium chamaedrys C. Koch

Cited by 31 publications

References 28 publications

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

Comparative study of genotoxicity and antimutagenicity of methanolic extracts from Teucrium chamaedrys and Teucrium montanum in human lymphocytes using micronucleus assay

Antimicrobial activity, total phenolic content and flavonoid concentrations of Teucrium species

STUDIES ON ANTIOXIDANT PROPERTIES OF THE DIFFERENT SOLVENT EXTRACTS AND FATTY ACID COMPOSITION OFHYOSCYAMUS RETICULATUSL.

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