2007
DOI: 10.1039/b614770d
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Determination of the antibacterial efficacy of a new porphyrin-based photosensitizer against MRSA ex vivo

Abstract: Following extensive in vitro screening of new photosensitizers the purpose of the present study was to examine penetration as well as antibacterial efficacy of a lead photosensitizer against MRSA using an ex vivo porcine skin model. Two different applications were performed: (i) preincubation of bacteria in solution with a porphyrin-based photosensitizer XF73 and subsequent application on the ex vivo porcine skin; (ii) application of pure bacteria on the explants followed by an incubation with XF73 in a water-… Show more

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Cited by 62 publications
(62 citation statements)
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“…For each skin sample a cotton-tipped rod was moistened in sampling buffer (0.1% Tween80, 0.0075 M phosphate puffer, pH 7.9) before swabbing the entire surface three times. First the recovery efficacy was tested to establish the range of viable numbers of CFU per ml of untreated skin samples, which was in accordance with already published data [16]. A reproducible correlation between three swabbing steps and the recovery efficiency of bacteria applied to the skin could be evaluated (data not shown).…”
Section: Methodssupporting
confidence: 57%
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“…For each skin sample a cotton-tipped rod was moistened in sampling buffer (0.1% Tween80, 0.0075 M phosphate puffer, pH 7.9) before swabbing the entire surface three times. First the recovery efficacy was tested to establish the range of viable numbers of CFU per ml of untreated skin samples, which was in accordance with already published data [16]. A reproducible correlation between three swabbing steps and the recovery efficiency of bacteria applied to the skin could be evaluated (data not shown).…”
Section: Methodssupporting
confidence: 57%
“…The preparation was done as already published [16]. Briefly, the excised skin was washed with water, epilated with a dry razor and the adipose tissue beneath the dermis was removed with a scalpel.…”
Section: Methodsmentioning
confidence: 99%
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“…A previous study [26] has suggested that the use of PACT seems to be a practical alternative for treating osteomyelitis. However, factors such as the pharmacokinetics of the PS, the use of an adequate wavelength and both irradiation and incubation protocols may affect the outcome of the procedure.…”
Section: Discussionmentioning
confidence: 98%
“…[148][149][150] XF73, a cationic porphyrin PS, is able to reduce MRSA growth by >3 log 10 in a porcine skin infection model. 151 PDT with polycationic PS conjugates and 665 nm light in murine excisional wounds led to a reduction in infectious organisms, permitting mouse survival to reach 90%, and reduce substantially pathogen viability. 152 TBO and light cured mice of otherwise fatal Vibrio vulnificus wound infections in murine models.…”
Section: Acknowledgmentsmentioning
confidence: 99%