Photodynamic Antimicrobial Chemotherapy (PACT) in osteomyelitis induced by Staphylococcus aureus: Microbiological and histological study

Reis, João José; Santos, Jean Nunes dos; Barreto, Brunna Santos; Assis, Patrícia Nascimento de; Almeida, Paulo Fernando de; Pinheiro, António Luiz Barbosa

doi:10.1016/j.jphotobiol.2015.06.005

Cited by 17 publications

(8 citation statements)

References 25 publications

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“…Recently, a clinical study was conducted to examine the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions A gram-positive cariogenic bacterial species, S. aureus was revealed to be the most commonly detected bacteria species in both periodontal and peri-implant sites, irrespective of their health status [55]. Antimicrobial chemotherapy treatment which aimed to reduce the number of S. aureus enabled a better quality of bone repair of tibial surgical bone defects in rats [56]. P. gingivalis is considered to be one of the most important microflora of dental peri-implantitis [57].…”

Section: Discussionmentioning

confidence: 99%

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

et al. 2018

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Section: Discussionmentioning

confidence: 99%

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

et al. 2018

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“…Such broad-spectrum potential is important given the polymicrobial nature of many clinical infections and would aid in local 'photoempirical' administration for wounds at the clinical triage stage, this being a logical application of photoantimicrobials, allowing conservation of conventional systemic drugs. The efficacy of TBO against bacteria in biofilms [17] and in trialling against suitable conditions/presentations in vivo, such as onychomycosis [18] and osteomyelitis [19] is also encouraging, both for clinical involvement outside dentistry and in the search for improved photoantimicrobial agents.…”

Section: Antimicrobial Efficacymentioning

confidence: 99%

Phenothiazinium photosensitisers XI. Improved toluidine blue photoantimicrobials

Wainwright

O'Kane

Rawthore

2016

Journal of Photochemistry and Photobiology B: Biology

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The phenothiazinium derivative toluidine blue O (TBO) is widely employed as a photoantimicrobial agent in clinical trialling, particularly in dentistry. However, its activity against a range of pathogenic microbial species is not significantly different to that of the standard photoantimicrobial methylene blue. In the current study, derivatives of TBO with varying hydrocarbon substitution in chromophore position 2 were synthesised via the established anilinethiosulphonic route, using the mild oxidant silver(II) carbonate to allow substituent preservation.The resulting series of analogues demonstrated the expected increases in visible absorption wavelength and lipophilicity with increasing hydrocarbon content, as well as decreased aggregation for derivatives with bulkier substituents, and all produced singlet oxygen on illumination in vitro. Screening against a range of bacterial and fungal pathogens relevant to infection control showed remarkable increases in activity relative to the parent compound, particularly against the clinically important Gram-negative bacterium Pseudomonas aeruginosa. In addition, in order to demonstrate clinical relevance, the photoactivities of the new derivatives against microbial targets were compared to conventional antibacterial and antifungal drugs, as well as biocides commonly used for local disinfection. Activity here was also generally greater than that of the conventional agents used for comparison, considerably so relative to the local disinfectant agents.

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“…In recent years, increased bacterial resistance to conventional antibiotics has become a worldwide public health problem, which emphasizes the need for new antibacterial agents to fight against the antibiotics-resistant bacteria . Photodynamic antimicrobial chemotherapy (PACT) has emerged as a promising modality to inactivate the pathogen bacteria via reactive oxygen species (ROS), which is cytotoxic to multidrug-resistant bacteria, and so far almost no resistance to ROS has been found. − It is also well-established that PACT exhibits excellent antibacterial activities through the oxidative damage of bacteria mainly by reactive oxygen species (ROS), including singlet oxygen and radical species, which are generated by energy transfer from the excited triplet of the photosensitizer to oxygen and the reaction of photosensitizer with substrate molecules, respectively . Porphyrins and their derivatives are widely employed as the photosensitizers in PACT research for the relatively high yield of ROS efficiency (in particular singlet oxygen, 1 O 2 ) and facile preparation. , However, their poor water solubility makes them easily aggregate in aqueous solutions, which leads to significant fluorescence quench and the reduction of PACT efficiency.…”

Section: Introductionmentioning

confidence: 99%

Polyhedral Oligomeric Silsesquioxane (POSS)-Based Cationic Conjugated Oligoelectrolyte/Porphyrin for Efficient Energy Transfer and Multiamplified Antimicrobial Activity

Chen

Shan

et al. 2018

ACS Appl. Mater. Interfaces

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Cationic quaternary ammonium (QA) groups and reactive oxygen species as two main approaches for antibacterial study have been intensively studied. Herein, we report a multifunctional antimicrobial agent (porphyrin-POSS-OPVE, PPO), which combines bacterial membrane intercalation, high density of local QA groups, efficient energy transfer, significantly reduced aggregation, and high water solubility into one single molecule. The light-harvesting PPO contains multiple donor-absorbing arms (oligo( p-phenylenevinylene) electrolytes, OPVEs) on its globular periphery and a central porphyrin acceptor in the core by using three-dimensional nanocages (polyhedral oligomeric silsesquioxanes, POSSs) as bridges. The antiaggregation ability of POSS and the highly efficient energy transfer from multiple OPVE arms to porphyrin could greatly amplify singlet oxygen generation in PPO. Particularly, OPVEs with QA terminal chains were able to intercalate into Escherichia coli membranes, which facilitated O diffusion and bacterial cell membrane disintegration by QA groups. The increased local cationic QA charges in OPVE arms can also enhance the biocidal activity of PPO. Benefiting from these satisfactory features, PPO exhibits multiamplified antibacterial efficacy under a very low concentration level and white light dose (400-700 nm, 6 mW·cm, 5 min, 1.8 J·cm) to Escherichia coli (8 μM) and Staphylococcus aureus (500 nM). Therefore, PPO shows great potential for photodynamic antimicrobial chemotherapy at a much lower irradiation light dose and photosensitizer concentration level compared to previous reports.

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Photodynamic Antimicrobial Chemotherapy (PACT) in osteomyelitis induced by Staphylococcus aureus: Microbiological and histological study

Cited by 17 publications

References 25 publications

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

Effects of different sterilization methods on surface characteristics and biofilm formation on zirconia in vitro

Phenothiazinium photosensitisers XI. Improved toluidine blue photoantimicrobials

Polyhedral Oligomeric Silsesquioxane (POSS)-Based Cationic Conjugated Oligoelectrolyte/Porphyrin for Efficient Energy Transfer and Multiamplified Antimicrobial Activity

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